DOI QR코드

DOI QR Code

Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

  • Choi, Go-Woon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, June-Pyo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Ju, Hye-Kyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hu, Wen-Xing (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Boram (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Jong-Seok (Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Domier, Leslie L (Department of Crop Sciences, University of Illinois at Urbana-Champaign) ;
  • Hammond, John (United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit) ;
  • Song, Kihak (Department of Urology, Chungnam National University School of Medicine) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2018.12.10
  • 심사 : 2019.06.10
  • 발행 : 2019.10.01

초록

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

키워드

참고문헌

  1. Bao, Y., Carter, S. A. and Nelson, R. S. 1996. The 126- and 183-kilodalton proteins of tobacco mosaic virus, and not their common nucleotide sequence, control mosaic symptom formation in tobacco. J. Virol. 70:6378-6383. https://doi.org/10.1128/JVI.70.9.6378-6383.1996
  2. Buck, K. W. 1999. Replication of Tobacco mosaic virus RNA. Philos. Trans. R. Soc. Lond. B Biol. Sci. 354:613-627. https://doi.org/10.1098/rstb.1999.0413
  3. Choi, G.-W., Kim, B., Ju, H., Cho, S., Seo, E., Kim, J., Park, J., Hammond, J. and Lim, H.-S. 2018. Dual infections of Tomato mosaic virus (ToMV) and Tomato yellow leaf curl virus (TYLCV), or Tomato mosaic virus (ToMV) and Tomato chlorosis virus (ToCV), detected in tomato fields located in Chungcheongnam-do in 2017. Korean J. Agric. Sci. 45:38-42. https://doi.org/10.7744/KJOAS.20180008
  4. Choi, H. S., Lee, S. H., Kim, M. K., Kwak, H. R., Kim, J. S., Cho, J. D. and Choi, G. S. 2010. Occurrence of virus diseases on major crops in 2009. Res. Plant Dis. 16:1-9 (in Korean). https://doi.org/10.5423/RPD.2010.16.1.001
  5. Csorba, T., Bovi, A., Dalmay, T. and Burgyan, J. 2007. The p122 subunit of Tobacco mosaic virus replicase is a potent silencing suppressor and compromises both small interfering RNAand microRNA-mediated pathways. J. Virol. 81:11768-11780. https://doi.org/10.1128/JVI.01230-07
  6. Han, S.-H., Park, J.-S., Han, J.-Y., Gong, J.-S., Park, C.-H., Kim, J.-K., Seo, E.-Y., Domier, L. L., Hammond, J. and Lim, H.-S. 2017. New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein. Virus Genes 53:434-445. https://doi.org/10.1007/s11262-017-1432-4
  7. Higuchi, R., Krummel, B. and Saiki, R.K. 1988. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16:7351-7367. https://doi.org/10.1093/nar/16.15.7351
  8. Ishibashi, K. and Ishikawa, M. 2014. Mechanisms of tomato mosaic virus RNA replication and its inhibition by the host resistance factor Tm-1. Curr. Opin. Virol. 9:8-13. https://doi.org/10.1016/j.coviro.2014.08.005
  9. Kim, I.-H., Han, J.-Y., Cho, I.-S., Ju, H. K., Moon, J. S., Seo, E.-Y., Kim, H. G., Hammond, J. and Lim, H.-S. 2017. Generation of an infectious clone of a new Korean isolate of Apple chlorotic leaf spot virus driven by dual 35S and T7 promoters in a versatile binary vector. Plant Pathol. J. 33:608-613. https://doi.org/10.5423/PPJ.NT.05.2017.0106
  10. Kubota, K., Tsuda, S., Tamai, A. and Meshi, T. 2003. Tomato mosaic virus replication protein suppresses virus-targeted posttranscriptional gene silencing. J. Virol. 77:11016-11026. https://doi.org/10.1128/JVI.77.20.11016-11026.2003
  11. Kurihara, Y., Inaba, N., Kutsuna, N., Takeda, A., Tagami, Y. and Watanabe, Y. 2007. Binding of tobamovirus replication protein with small RNA duplexes. J. Gen. Virol. 88(Pt. 8):2347-2352.
  12. Park, C.-H., Ju, H.-K., Han, J.-Y., Park, J.-S., Kim, I.-H., Seo, E.-Y., Kim, J.-K., Hammond, J. and Lim, H.-S. 2017. Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters. Virus Genes 53:286-299. https://doi.org/10.1007/s11262-016-1415-x
  13. Procter, C. H. and Fry, P. R. 1965. Seed transmission of tobacco mosaic virus in tomato. N. Z. J. Agric. Res. 8:367-369. https://doi.org/10.1080/00288233.1965.10422368
  14. Wang, L.-Y., Lin, S.-S., Hung, T.-H., Li, T.-K., Lin, N.-C. and Shen, T.-L. 2012. Multiple domains of the Tobacco mosaic virus p126 protein can independently suppress local and systemic RNA silencing. Mol. Plant-Microbe Interact. 25:648-657. https://doi.org/10.1094/MPMI-06-11-0155