Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Changes in Vitamins (BB1, B7, B12) and Specific Bacteria on the Growth Stages of Marine Diatom Cyclotella meneghiniana

해양 규조류 Cyclotella meneghiniana의 성장단계에 따른 비타민(B1, B7, B12) 및 특이적 미생물의 변동

  • Choi, Won-Ji (Department of Biotechnology, Biochemical Engineering, Sangmyung University) ;
  • Ki, Jang-Seu (Department of Biotechnology, Biochemical Engineering, Sangmyung University)
  • 최원지 (상명대학교 생명화학공학부 생명공학과) ;
  • 기장서 (상명대학교 생명화학공학부 생명공학과)
  • Received : 2019.08.01
  • Accepted : 2019.09.09
  • Published : 2019.09.30
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Abstract

Diatom growth is affected by associated bacteria that probably provide useful substances like vitamins. In the present study, we analysed the variation of vitamins $B_1$, $B_7$ and $B_{12}$ on the growth stages of the marine diatom Cyclotella meneghiniana and assessed putative vitamin-producing bacteria (e.g., ${\alpha}$- and ${\gamma}$-proteobacteria). HPLC analysis showed that total amounts of vitamins $B_1$ and $B_{12}$ decreased with cell growth, whereas vitamin $B_7$ increased gradually on the growth stages. $B_1$ and $B_{12}$ measured 0.5% and 0.18% at the stationary phase, following 0.25% and 0.72% at the lag phase. They considerably increased to 0.75% and 0.77% at the death stage. 16S pyrosequencing showed relatively high ratios of ${\alpha}$- and ${\gamma}$-proteobacteria in all the growth stages of the C. meneghiniana. In addition, we detected previously-reported vitamin-producing bacteria, such as Marinobacter, in high numbers. The species was dorminant in the lag (relative abundance 72%) and exponetial (72%) stages, whareas it decreased in the stationary (49%) and death (48%) stages. These results suggest that vitamins $B_1$ or $B_{12}$ may be necesaary for diatom growth and that associated bacteria, including Marinobacter, may produce these substances for the cell growth of C. meneghiniana.

Keywords

References

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