• Ocean and Polar Research
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• v.39 no.4
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• pp.245-255
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• 2017

There are a number of pieces of evidences that suggest a link between marine diatoms and microorganisms, but knowledge about related microbial communities is greatly lacking. The present study investigated the microbial community structures related to the growth of the marine diatom Cyclotella meneghiniana. We collected free-living bacteria (FLB) and particle-associated bacteria (PAB) at each growth stage (e.g., lag, exponential, stationary and death) of the diatom, and analyzed their bacterial 16S rDNA using pyrosequencing. Metagenomics analysis showed that community structures of FLB and PAB differed considerably with the progress of growth stages. FLB showed higher diversity than PAB, but variation in the different growth stages of C. meneghiniana was more evident in PAB. The proportion of the genus Hoeflea, belonging to the order Rhizobiales, was dominant in both FLB and PAB, and it gradually increased with the growth of C. meneghiniana. However, Enhydrobacter clade tended to considerably decrease in PAB. In addition, Marinobacter decreased steadily in FLB, but first increased and then decreased in PAB. These results suggest that Hoeflea, Enhydrobacter, and Marinobacter may be closely related to the growth of diatom C. meneghiniana.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.6
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• pp.430-435
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• 2013

A seasonal variation of phytoplankton community in the middle-lower part of the Nakdong River was studied in four sampling stations at monthly intervals from January to December 2012. We identified 40 genera 72 species of phytoplankton. Among these, diatoms were 36 species (50.0%), green algae 20 species (27.8%), blue-green algae 9 species (12.5%) and others 7 species (9.7%), respectively. The phytoplankton standing crops were recorded a maximum of 29,640 cells/mL at the Mulgeum (St. 4) in June and a minimum of 236 cells/mL at the Goryung (St. 1) in October. Also, Standing crops were increased with proceeding from middle part to lower part. Ecological important species were Stephanodiscus hantzschii, Cyclotella meneghiniana, Synedra acus, Aulacoseira granulata, Pediastrum sp. and Microcystis aeruginosa. Seasonal succession of phytoplankton represented that Stephanodiscus hantzschii was dominant species in winter, Cyclotella meneghiniana, Synedra acus was dominant species in spring, Microcystis aeruginosa, Cyclotella meneghiniana, Fragilaria crotonensis, Synedra acus, Aulacoseria granulata was dominant species in summer, and Aulacoseria granulata, Cyclotella meneghiniana, Fragilaria crotonensis was dominant species in autumn. In the community analysis, diversity index and dominant index were higher May~July and December~February, respectively. Also, diversity index and dominant index were decreased and increased with proceeding from middle part to lower part.

• Ocean and Polar Research
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• v.41 no.3
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• pp.203-211
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• 2019

Diatom growth is affected by associated bacteria that probably provide useful substances like vitamins. In the present study, we analysed the variation of vitamins $B_1$, $B_7$ and $B_{12}$ on the growth stages of the marine diatom Cyclotella meneghiniana and assessed putative vitamin-producing bacteria (e.g., ${\alpha}$- and ${\gamma}$-proteobacteria). HPLC analysis showed that total amounts of vitamins $B_1$ and $B_{12}$ decreased with cell growth, whereas vitamin $B_7$ increased gradually on the growth stages. $B_1$ and $B_{12}$ measured 0.5% and 0.18% at the stationary phase, following 0.25% and 0.72% at the lag phase. They considerably increased to 0.75% and 0.77% at the death stage. 16S pyrosequencing showed relatively high ratios of ${\alpha}$- and ${\gamma}$-proteobacteria in all the growth stages of the C. meneghiniana. In addition, we detected previously-reported vitamin-producing bacteria, such as Marinobacter, in high numbers. The species was dorminant in the lag (relative abundance 72%) and exponetial (72%) stages, whareas it decreased in the stationary (49%) and death (48%) stages. These results suggest that vitamins $B_1$ or $B_{12}$ may be necesaary for diatom growth and that associated bacteria, including Marinobacter, may produce these substances for the cell growth of C. meneghiniana.

• Journal of Ecology and Environment
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• v.36 no.4
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• pp.407-419
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• 2013

Cyclotella species were collected at 51 sites from July 2010 to June 2013 in Korean coastal waters. A total of five Cyclotella species (C. atomus var. marina, C. baltica, C. litoralis, C. meduanae, and C. meneghiniana) were identified in this study. The diagnostic characteristics for five Cyclotella species are described, focusing on the spacing, position, number of satellite pores of the mantle fultoportula and valve face fultoportula. In addition, we put the salinity ranges of five species of Cyclotella together. Of the five Cyclotella species, C. baltica, C. litoralis and C. meduanae are newly recorded in Korea.

• Journal of Environmental Science International
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• v.10 no.1
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• pp.41-45
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• 2001

This study was carried out to investigate phytoplankton community structure in the Naktong river from January to December in 1999. In water quality, average value of pH were 8.1, BOD 2.5mg/$\ell$, COD 5.0mg/$\ell$, Chl-a 41$\mu\textrm{g}$/$\ell$, and $NH_4^+-N$ 0.08mg/$\ell$, respectively. Phytoplankton were identified 42 genera 76 species. Among these, diatoms were 39 species(51.3%), green algae 25 species(32.9%), cyanobacteria 4 species(5.3%), dinoflagellates 4 repectively. The highest standing crops were 33,023 cells/$m\ell$ in February at the Mulgum and the lowest 79 cells/$m\ell$ in March at the Goryung. Also, Standing crops were increased with proceeding from middle part to lower part. Seasonal succession of phytoplankton represented that Stephanodiscus hantzschii was dominant species in winter, Cyclotella menaghiniana and Synedra acus in spring, C. meneghiniana, S. acus, Aulacoseira granulata var. angustissima in summer, and A. granulata var. angustissima and C. meneghiniana in autumn. Ecological important species were 4 species, that are Stephanodiscus hantzschii, Cyclotella meneghiniana, Synedra acus, and Aulacoseira granulata var. angustissima. In the community analysis, dominance indices ranged from 0.434(August, Namji) to 0.999(January, February, Mulgum) and diversity indices from 0.026(February, Mulgum) to 3.073(September, Namji). According to the similarity index among the stations, it was generally defind as two areas such as middle(Goryung, Namji and Samryangjin) and lower part(Mulgum).

• Korean Journal of Ecology and Environment
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• v.47 no.4
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• pp.273-281
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• 2014

We conducted the weekly monitoring (December 2012~April 2013) to evaluate the temporal variation and identification of Stephanodiscus spp. that are generally dominant during winter in the Yeongsan River. Phytoplankton species were identified and counted using the optical microscope and scanning electron microscope (SEM). Phytoplankton in the river were grouped into 6 classes (bacillariophyceae, chlorophyceae, cryptophyceae, cyanophyceae, dinophyceae, euglenophyceae), 30 genus and 41 species. Phytoplankton composition showed high abundance of diatoms in winter and Aulacoseira sp., Cyclotella sp. and Stephanodiscus spp. were dominant. Among the species, Stephanodiscus spp. was relatively abundant compared to other diatom species. Stephanodiscus spp. appeared from December 2012 to April 2013 and their abundance peaked in January. Abundance of diatoms especially peaked ($21,080cells\;mL^{-1}$) in January 15, 2013 when Stephanodiscus spp. also bloomed ($20,560cells\;mL^{-1}$). The abundances of Stephanodiscus spp. were gradually decreased from March and reached as low as $60cells\;mL^{-1}$ in April 26. Cyclostephanos (C. invisitatus), Cyclotella (C. meneghiniana), Discostella (D. pseudostelligera, D. woltereckii) and Stephanodiscus (S. hantzschii, S. minutulus, S. parvus) were classified in the circular diatoms. Abundance of S. hantzschii was extremely high compared to S. minutulus and S. parvus.

• Journal of Environmental Science International
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• v.7 no.5
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• pp.607-613
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• 1998

This study was carried out to investigate flora of phytoplankton in Milyang River from July 1996 to April 1997. Phytoplankton were identified 206 kinds and composed of 2 var.-for., 5 forms, 35 varieties, 164 species, 70 genera, 31 families, 14 orders, 6 classes and 5 phyla. According to the seasonal variation, 131 species were founded in summer, 109 species in spring, 108 species in autumn, and 100 species in winter, respectively. Seasonal and stationary variation of standing crops were between 10 and 5.600 cells/mι. At station 7, Cyclotella meneghiniana was bloomed 5,000 cells/mι(89.7%) in winter, and Stephanodiscus hantzschii was bloomed 3,400 cells/mι(74.3%) in spring. The number of species and standing crops were increased with proceeding from upper stream to lower stream. Important species of phytoplankton were 24 species, that are 4 species of Cyanophyta (Aphanocapsa elachista, Merismopedium glaucum, Lyngbya limnetica, Oscillatoria tenuis), 12 species of Crysophyta (Melosira varians, Stephanodiscus hantzschii, Cyclotalla meneghiniana, Fragilaria construens var. venter, Navicula cryptocephala, Cymbella ventricosa, Gomphonema olivaceum), and 8 species of Chlorophyta (Chlamydomonas reinhardi, Eudorina elegans, Pandorina morum, Oocystis borgei, Scenedesmus quadricauda, Microspora crassior). According to the similarity index among the stations, it was generally defind as two water areas such as upper stream(station 1~3) and lower stream(station 4~7).

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.588-595
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• 2013

This study investigated the influence of temperature and $CO_2$ increase on phytoplankton growth and community structure during cold water season (spring) in Lake Paldang, Korea. Four experimental treatments of temperature and $CO_2$ manipulation were prepared in the laboratory batch culture: (1) Control; ambient low temperature ($6{\pm}2^{\circ}C$) and low $CO_2$ (air level, $400mgL^{-1}$), (2) T1; low temperature and high $CO_2$ ($800mgL^{-1}$), (3) T2; high temperature ($20{\pm}2^{\circ}C$) and low $CO_2$, (4) T3; high temperature and high $CO_2$. Algal growth experiment was carried out for 10 days under the light intensity of $70{\mu}mol\;m^{-2}s^{-1}$ (L :D=24 : 0). The level of pH decreased in both T1 and T3, due to dissolution of added $CO_2$. The dominant phytoplankton species of ambient water, Cyclotella meneghiniana succeeded to Fragilaria capucina var. gracilis in high-temperature treatment groups (T2 and T3). Cyanobacteria were very rare at the beginning of the experiment, while Oscillatoria limnetica appeared in only high-temperature groups (T2 and T3) at $6{\sim}7^{th}$ day. $CO_2$ addition in ambient temperature (T1) induced the highest phytoplankton growth, and thereby producing the highest average cell density of $3.27{\pm}0.33\;10^4\;cells\;mL^{-1}$, followed by T2 ($2.65{\pm}0.26\;10^4\;cells\;mL^{-1}$), T3 ($2.09{\pm}0.16\;10^4\;cells\;mL^{-1}$), and Control ($1.86{\pm}0.13\;10^4\;cells\;mL^{-1}$) (F=7.167, p=0.000). In summary, temperature increase changed the phytoplankton community structure and $CO_2$ increase promoted the phytoplankton growth during the cold spring season in Lake Paldang, suggesting a potential effect of climate change on freshwater phytoplankton.

• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.191-199
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• 2003

This study was conducted to investigate a seasonal variation of phytoplankton communities at 6 stations in the Hantan River from November, 2001 to August, 2002. Electric conductivity gradually increased from the upper reaches to the down reaches. DO differed not much from the upper reaches to down reaches, but it decreased at the station where the pollutants inflowed. T-N and T-P concentrations were high at station 4${\sim}$6. Chlorophyll-a concentration gradually increased at the mid and down reaches. It was high in May but decreased in August by the heavy rains. Phytoplankton communities were identified a total 354 taxa composed of 99 genus, 320species, 19 varieties, 3 forma and 12 unidentified species. There were 135 taxa of Chlorophyceae, 134 taxa of Bacillariophyceae, and the other taxa were Euglenophyceae, Cyanophyceae, Xanthophycaea, Chrysophyceae and Dinophyceae. Standing crops ranged from 8,600 to 337,100 cells/mL and it was lower in August than May by the rains. Dominant species included Achnanthes alteragracillima, Achnanthes convergens, Achnanthes minutissima and Cymbella minuta var. silesiaca which were the saproxenous species at the reaches, and were Cyclotella meneghiniana, Chlamydomonas pseudopertyi and Nitzschia which were saprophilous and eutrophic species at the down reaches. The correlation coefficients between phytoplankton standing crops and EC, BOD and T-N, T-P was 0.68, 0.60, 0.60 and 0.70, respectively. All correlation coefficients between Chlorophyceae and EC, BOD and T-P were higher than 0.6. A. convergens were negative correlations with EC and BOD, but N. palea were positive with EC, BOD, T-N and T-P.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.10 no.3
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• pp.154-163
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• 2005

Irregular discharges of freshwater through the water gates of the Keum River Estuarine Weir, Korea, whose construction had been completed in 1998 with its water gates being operated as late as August 1994, drastically modified the estuarine environment. Sharp decrease of salinity along with the altered concentrations of inorganic nutrients are accompanied with the irregular discharges of freshwater into the estuary under the influence of regular semi-diurnal tidal effect. Field sampling was carried out on the time of high tide at 2 fixed stations(St.1 near the Estuarine Weir and St.2 off Kunsan Ferry Station) every other day for 4 months from mid-February 2004 to investigate into the semi-weekly variation of spring phytoplankton community in relation to the freshwater discharges from Keum River Estuarine Weir. CV(coefficient of variation) of salinity measurements was roughly 2 times greater in St.1 than that in St.2, reflecting extreme salinity variation in St.1 Among inorganic nutrients, concentrations of N-nutrients($NO_3^-,\;NO_2^-$ and $NH_4^+$) were clearly higher in St.1, to imply the more drastic changes of the nutrient concentrations in St.1. than St.2 following the freshwater discharges. As a component of phytoplankton community, diatoms were among the top dominants in terms of species richness as well as biomass. Solitary centric diatom, Cyclotella meneghiniana, and chain-forming centric diatom, Skeletonema costatum, dominated over the phytoplankton community in order for S-6 weeks each (Succession Interval I and II), and the latter succeeded to the former from the time of <$10^{\circ}C$ of water temperature. Cyanobacterial species, Aphanizomenon Posaquae and Phormidium sp., which might be transported into the estuary along with the discharged freshwater, occupied high portion of total biomass during Succession Interval III(mid-April to late-May). During this period, freshwater species exclusively dominated over the phytoplankton community except the low concentrations of the co-occurring 2 estuarine diatoms, Cyclotella meneghiniana and Skeletonema costatum. During the 4th Succession Interval when the water temperature was over $18^{\circ}C$, the diatom, Guinardia delicatula, was predominant for a week with the highest dominance of $75\%$ in discrete samples. To summarize, during all the Succession Intervals other than Succession Interval III characterized by the extreme variation of salinity under cooler water temperature than $18^{\circ}C$, the diatoms were the most important dominants for species succession in spring. If the scale and frequency of the freshwater discharge could have been adjusted properly even during the Succession Interval III, the dominant species would quite possibly be replaced by other estuarine diatom species rather than the two freshwater cyanobacteria, Aphanizomenon flosaquae and Phormidium sp.. The scheme of field sampling every other day for the present study was concluded to be the minimal requirement in order to adequately explore the phytoplankton succession in such estuarine environment as in Keum River Estuary: which is stressed by the unpredictable and unavoidable discharges of freshwater under the regular semi-diurnal tide.