Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antioxidant Effect of Anserine Extracted from Salmon (Oncorhynchus keta)

연어(Oncorhynchus keta) 추출 anserine의 항산화 효과

  • Min, Hye-Ok (Ministry of Food and Drug Safety Food Safety Policy Bureau Health Functional Food Policy Division) ;
  • Song, Ho-Su (Department of Western Cuisine and Culinary Arts, Youngsan University)
  • 민혜옥 (식품의약품안전처 식품안전정책국 건강기능식품정책과) ;
  • 송호수 (영산대학교 서양조리학과)
  • Received : 2019.07.12
  • Accepted : 2019.07.17
  • Published : 2019.08.30
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Abstract

Ion-exchange chromatography and ultrafiltration were used to extract anserine from salmon (Oncorhynchus keta). The salmon anserine showed DPPH radical scavenging activity in the range of 7.30% to 31.05% in a dose-dependent manner. This reducing power of salmon anserine also increased as the concentration increased. Metal chelate activity, superoxide dismutase - like activity, and thiobarbituric acid reactive substances assay showed similar results. The anserine also suppressed the increment of the peroxide value and linoleic acid during storage periods. These results suggest that salmon anserine might be useful as a natural antioxidant in various foodstuffs.

연어로부터 추출한 anserine의 항산화능 평가를 위해 1차 이온교환 후 한외여과 및 2차 이온교환 추출물인 IEC-B 실험구와 합성 anserine, ${\beta}-alanine$, 1-methylhistidine, taurine, ascorbic acid, BHT를 대조구로 DPPH 라디칼 소거능, 환원력, 금속 킬레이트능, SOD 유사활성 실험을 하였다. DPPH 라디칼 소거능 결과 대조구와 anserine 모두 농도 증가에 따라 라디칼 소거능이 증가하였으며 BHT가 가장 높은 소거능을 나타냈다. 합성 anserine은 농도 증가에 따라 $9.30{\pm}0.65{\sim}28.23{\pm}0.24%$를 나타냈으며 연어 추출 anserine인 IEC-B 추출물은 $7.30{\pm}1.13{\sim}31.05{\pm}0.17%$를 나타내 BHT, ascorbic acid에 비해 소거능이 낮으나 두 실험구와 비교했을 때 약 37%, 49% 정도의 소거능을 나타냈다. 환원력 또한 첨가농도에 따라 증가하였으며 BHT와 ascorbic acid가 높은 환원력을 나타냈고, 그 외 실험구 중에서는 합성 anserine과 연어 추출 anserine인 IEC-B 실험구가 높았으며 유의적으로 동일한 환원력을 나타냈다. 금속 킬레이트능은 BHT, ascorbic acid 순으로 높았으며 합성 anserine과 연어 추출 anserine의 경우 ascorbic acid와 유사한 금속 킬레이트능을 나타냈다. 또한 SOD 유사활성에서는 연어 추출 anserine이 BHT와 ascorbic acid에 비해 낮았으나 농도 증가에 따라 증가하는 경향을 나타내었으며, 합성 anserine, 연어 추출 anserine, ascorbic acid, BHT를 이용하여 과산화물가와 TBARS를 실험하였다. 저장기간 중 linoleic acid의 과산화물가는 큰 폭으로 증가한 반면 연어 추출 anserine을 첨가한 경우 과산화물가의 증가가 억제되는 경향이 나타났으며, 이는 TBARS 실험 결과와 유사한 경향을 나타냈다.

Keywords

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