Mycobiology

The Korean Society of Mycology (한국균학회)
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Sulzbacheromyces sinensis, an Unexpected Basidiolichen, was Newly Discovered from Korean Peninsula and Philippines, with a Phylogenetic Reconstruction of Genus Sulzbacheromyces

  • Liu, Dong (Korean Lichen Research Institute (KoLRI), Sunchon National University) ;
  • Wang, Xin Yu (Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany, Chinese Academy of Sciences) ;
  • Wang, Li Song (Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany, Chinese Academy of Sciences) ;
  • Maekawa, Nitaro (Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University) ;
  • Hur, Jae-Seoun (Korean Lichen Research Institute (KoLRI), Sunchon National University)
  • Received : 2019.01.17
  • Accepted : 2019.05.07
  • Published : 2019.06.01
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Abstract

Most of lichens are formed by Ascomycota, less than 1% are lichenized Basidiomycota. The flora investigation of lichenized Ascomycota of South Korea has been well studied in the past three decades; however, prior to this study, none of basidiolichens was discovered. During the recent excursion, an unexpected clavarioid basidiolichen, Sulzbacheromyces sinensis was collected. Morphology and ecology has been recorded in detail. DNA was extracted, and ITS, 18S, 28S nuclear rDNA were generated. In order to further confirm the systematic position of the Korean specimens, maximum likelihood and Bayesian inference analysis including all the species of the order Lepidostromatales were conducted based on the ITS. As a result, the phylogenetic tree of the order Lepidostromatales was reconstructed, which differed from the previous studies. The inferred phylogenetic tree showed that species of Sulzbacheromyces in three different continents (Asia, South Africa and South America) were separated into three clades with support. In this study, the species worldwide distribution map of Lepidostromatales was illustrated, and S. sinensis had a widest distribution range (paleotropical extend to the Sino-Japanese) than other species (paleotropical or neotropical). Prior to this study, the range of distribution, southernmost and northernmost points and the fruiting time of S. sinensis were recorded, and the genus Sulzbacheromyces was firstly reported from Korean peninsula and Philippines.

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References

  1. Spribille T, Tuovinen V, Resl P, et al. Basidiomycete yeasts in the cortex of ascomycete macrolichens. Science. 2016;353:488-492. https://doi.org/10.1126/science.aaf8287
  2. Lucking R, Hodkinson BP, Leavitt SD. The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota - Approaching one thousand genera. Bryologist. 2016;119:361-416. https://doi.org/10.1639/0007-2745-119.4.361
  3. Liu D, Goffinet B, Ertz D, et al. Circumscription and phylogeny of the Lepidostromatales (lichenized Basidiomycota) following discovery of new species from China and Africa. Mycologia. 2017;109:730-748. https://doi.org/10.1080/00275514.2017.1406767
  4. Lucking R, Dal-Forno M, Sikaroodi M, et al. A single macrolichen constitutes hundreds of unrecognized species. Proc Natl Acad Sci USA. 2014; 111:11091-11096. https://doi.org/10.1073/pnas.1403517111
  5. Lawrey JD, Lucking R, Sipman HJ, et al. High concentration of basidiolichens in a single family of agaricoid mushrooms (Basidiomycota: Agaricales: Hygrophoraceae). Mycol Res. 2009;113:1154-1171. https://doi.org/10.1016/j.mycres.2009.07.016
  6. Dal Forno M, Bungartz F, Yanez-Ayabaca A, et al. High levels of endemism among Galapagos basidiolichens. Fungal Divers. 2017;85:45-73. https://doi.org/10.1007/s13225-017-0380-6
  7. Liu D, Goffinet B, Wang X-Y, et al. Another lineage of basidiolichen in China, the genera Dictyonema and Lichenomphalia (Agaricales: Hygrophoraceae). Mycosystema. 2018;37:849-864.
  8. Petersen RH, Zang M. New or interesting clavarioid fungi from Yunnan, China. Acta Botanica Yunnanica. 1986;8:281-294.
  9. Petersen RH. Notes on clavarioid fungi. VII. Redefinition of the Clavaria vernalis-C. mucida complex. Am Midl Nat. 1967;77:205-221. https://doi.org/10.2307/2423440
  10. Hodkinson BP, Moncada B, Lucking R. Lepidostromatales, a new order of lichenized fungi (Basidiomycota, Agaricomycetes), with two new genera, Ertzia and Sulzbacheromyces, and one new species, Lepidostroma winklerianum. Fungal Divers. 2014;64:165-179. https://doi.org/10.1007/s13225-013-0267-0
  11. Hodkinson BP, Uehling JK, Smith ME. Lepidostroma vilgalysii, a new basidiolichen from the New World. Mycol Progress. 2012;11:827-833. https://doi.org/10.1007/s11557-011-0800-z
  12. Ertz D, Lawrey JD, Sikaroodi M, et al. A new lineage of lichenized basidiomycetes inferred from a two-gene phylogeny: the Lepidostromataceae with three species from the tropics. Am J Bot. 2008;95:1548-1556. https://doi.org/10.3732/ajb.0800232
  13. Sulzbacher MA, Wartchow F, Ovrebo CL, et al. Sulzbacheromyces caatingae: notes on its systematics, morphology and distribution based on ITS barcoding sequences. Lichenologist. 2016;48:61-70. https://doi.org/10.1017/S0024282915000420
  14. Sulzbacher MA, Baseia IG, Lucking R, et al. Unexpected discovery of a novel basidiolichen in the threatened Caatinga biome of northeastern Brazil. Bryologist. 2012;115:601-609. https://doi.org/10.1639/0007-2745-115.4.601
  15. He G, Wang PR, Chen SL, et al. An unexpected discovery of clavarioid fungi: first record of Lepidostroma asianum in China. Mycoscience. 2016;57:150-155. https://doi.org/10.1016/j.myc.2015.12.006
  16. Yanaga K, Sotome K, Suhara H, et al. A new species of Lepidostroma (Agaricomycetes, Lepidostromataceae) from Japan. Mycoscience. 2015;56:1-9. https://doi.org/10.1016/j.myc.2014.01.011
  17. Coca LF, Lucking R, Moncada B. Two new, sympatric and semi-cryptic species of Sulzbacheromyces (lichenized Basidiomycota, Lepidostromatales) from the Choco Biogeographic Region in Colombia. Bryologist. 2018;121:297-305. https://doi.org/10.1639/0007-2745-121.3.297
  18. Hur JS, Koh YJ, Harada H. A checklist of Korean lichens. Lichenology. 2005;4:66-95.
  19. Joshi Y, Koh YJ, Hur J-S. Three new records of lichen genus Rhizocarpon from South Korea. Mycobiology. 2010;38:219-221. https://doi.org/10.4489/MYCO.2010.38.3.219
  20. Moon KH, Aptroot A. Pyrenocarpous lichens in Korea. Biblio Lichenol. 2009;99:297-314.
  21. Aptroot A, Moon KH. 114 New reports of microlichens from Korea, including the description of five new species, show that the microlichen flora is predominantly Eurasian. Herzogia. 2014;27:347-365. https://doi.org/10.13158/heia.27.2.2014.347
  22. Moon KH. Lichen-forming and lichenicolous fungi of Korea. Incheon, Korea: National institute of biological resources; 2013. (In Korean)
  23. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York, US: Academic Press; 1990; p. 315-322.
  24. Rehner SA, Samuels GJ. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res. 1994;98:625-634. https://doi.org/10.1016/S0953-7562(09)80409-7
  25. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172:4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  26. Kauff F, Lutzoni F. Phylogeny of the Gyalectales and Ostropales (Ascomycota, Fungi): among and within order relationships based on nuclear ribosomal RNA small and large subunits. Mol Phylogen Evol. 2002;25:138-156. https://doi.org/10.1016/S1055-7903(02)00214-2
  27. Gargas A, Taylor JW. Polymerase chain reaction (PCR) primers for amplifying and sequencing nuclear 18s rDNA from lichenized fungi. Mycologia. 1992;84:589-592. https://doi.org/10.1080/00275514.1992.12026182
  28. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870-1874. https://doi.org/10.1093/molbev/msw054
  29. Katoh K, Standley DM. A simple method to control over-alignment in the MAFFT multiple sequence alignment program. Bioinformatics. 2016;32:1933-1942. https://doi.org/10.1093/bioinformatics/btw108
  30. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30:1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  31. Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001;17:754-755. https://doi.org/10.1093/bioinformatics/17.8.754
  32. Posada D. jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008;25:1253-1256. https://doi.org/10.1093/molbev/msn083
  33. Kauserud H, Heegaard E, Halvorsen R, et al. Mushroom's spore size and time of fruiting are strongly related: is moisture important? Biol Lett. 2011;7:273-276. https://doi.org/10.1098/rsbl.2010.0820