한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제23권4호
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  • Pages.333-344
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  • 2019
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Transcriptional Activity of an Estrogen Receptor β Subtype in the Medaka Oryzias dancena

  • Maeng, Sejung (Dept. of Marine Molecular Biosciences, Gangneung-Wonju National University) ;
  • Yoon, Sung Woo (Dept. of Marine Molecular Biosciences, Gangneung-Wonju National University) ;
  • Kim, Eun Jeong (Dept. of Marine Bio-Materials and Aquaculture, Pukyong National University) ;
  • Nam, Yoon Kwon (Dept. of Marine Bio-Materials and Aquaculture, Pukyong National University) ;
  • Sohn, Young Chang (Dept. of Marine Molecular Biosciences, Gangneung-Wonju National University)
  • 투고 : 2019.09.10
  • 심사 : 2019.09.29
  • 발행 : 2019.12.31
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초록

In vertebrate reproductive system, estrogen receptor (ER) plays a pivotal role in mediation of estrogenic signaling pathways. In the present study, we report the cDNA cloning, expression analysis, and transcriptional activity of ERβ1 subtype from medaka Oryzias dancena. The deduced O. dancena ERβ1 (odERβ1; 519 amino acids) contained six characteristic A/B to E/F domains with very short activation function 2 region (called AF2). A phylogenetic analysis indicated that odERβ1 was highly conserved among teleost ERβ1 subgroup. A conventional RT-PCR revealed that the odERβ1 transcripts were widely distributed in the multiple tissues, the ovary, brain, gill, intestine, kidney, and muscle. Further, the relatively higher odERβ1 expressions in the ovary and brain were clearly reproduced in RT-qPCR assay. When HA-fused odERβ1 expression vector was transfected into HEK293 cells, an immunoreactivity for odERβ1 was mainly detected in the nucleus part. Finally, an estrogen responsive element driven luciferase reporter assays demonstrated that the transcriptional activity of odERβ1 significantly increased by estradiol-17β (E2) in a dose dependent manner (p<0.05). However, fold-activation of odERβ1 in the presence of E2 was markedly weak, when it compared with those of O. latipes ERβ1. Taken together, these data suggest that odERβ1 represents a functional variant of teleost ERβ subtype and provides a basic tool allowing future studies examining the function of F domain of ERβ1 subtype and expanding our knowledge of ERβ evolution.

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참고문헌

  1. Antal MC, Petit-Demouliere B, Meziane H, Chambon P, Krust A (2012) Estrogen dependent activation function of $ER{\beta}$ is essential for the sexual behavior of mouse females. Proc Natl Acad Sci USA 109:19822-19827. https://doi.org/10.1073/pnas.1217668109
  2. Chakraborty T, Shibata Y, Zhou LY, Katsu Y, Iguchi T, Nagahama Y (2011) Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes. Mol Cell Endocrinol 333:47-54. https://doi.org/10.1016/j.mce.2010.12.002
  3. Cho YS, Lee SY, Kim YK, Kim DS, Nam YK (2011) Functional ability of cytoskeletal $\beta$-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena. Transgenic Res 20:1333-1355. https://doi.org/10.1007/s11248-011-9501-2
  4. Cho YS, Kim DS, Nam YK (2013) Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogenous choriogenin H promoter. Transgenic Res 22:501-517. https://doi.org/10.1007/s11248-012-9650-y
  5. Evans RM (1988) The steroid and thyroid hormone receptor superfamily. Science 240:889-895. https://doi.org/10.1126/science.3283939
  6. Glass CK, Rosenfeld MG (2000) The coregulator exchange in transcriptional functions of nuclear receptors. Genes Dev 14:121-141.
  7. Haldosen LA, Zhao C, Dahlman-Wright K (2014) Estrogen receptor beta in breast cancer. Mol Cell Endocrinol 382:665-672. https://doi.org/10.1016/j.mce.2013.08.005
  8. Jones DT, Taylor WR, Thornton JM (1992) The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 8:275-282.
  9. Jung DJ, Lee SK, Lee JW (2001) Agonist-dependent repression mediated by mutant estrogen receptor alpha that lacks the activation function 2 core domain. J Biol Chem 276:37280-37283. https://doi.org/10.1074/jbc.M106860200
  10. Kayo D, Zempo B, Tomihara S, Oka Y, Kanda S (2019) Gene knockout analysis reveals essentiality of estrogen receptor ${\beta}1$ (Esr2a) for female reproduction in medaka. Sci Rep 9:8868. https://doi.org/10.1038/s41598-019-45373-y
  11. Kim HK, Kim JH, Baek HJ, Kwon JY (2016) Gene expression of aromatases, steroid receptor, GnRH and GTHs in the brain during the formation of ovarian cavity in red spotted grouper, Epinephelus akaara. Dev Reprod 20:367-377. https://doi.org/10.12717/DR.2016.20.4.367
  12. Lange IG, Hartel A, Meyer HHD (2002) Evolution of oestrogen functions in vertebrates. J Steroid Biochem Mol Biol 83:219-226. https://doi.org/10.1016/S0960-0760(02)00225-X
  13. Lee Pow CSD, Yost EE, Aday DD, Kullman SW (2016) Sharing the roles: An assessment of Japanese medaka estrogen receptors in vitellogenin induction. Environ Sci Technol 50:8886-8895. https://doi.org/10.1021/acs.est.6b01968
  14. Maeng S, Jung Y, Choi E, Jeon JK, Kim S, Gen K, Sohn YC (2005) Expression of gonadotropin subunit genes following 4-nonylphenol exposure in masu salmon: Effects on transcript levels and promoter activities via estrogen receptor alpha. Comp Biochem Physiol B Biochem Mol Biol 142:383-390. https://doi.org/10.1016/j.cbpb.2005.08.009
  15. Mu WJ, Wen HS, Shi D, Yang YP (2013) Molecular cloning and expression analysis of estrogen receptor betas ($ER{\beta}1$ and $ER{\beta}2$) during gonad development in the Korean rockfish, Sebastes schlegeli. Gene 523:39-49. https://doi.org/10.1016/j.gene.2013.03.109
  16. Nagler JJ, Cavileer T, Sullivan J, Cyr DG, Rexroad C (2007) The complete nuclear estrogen receptor family in the rainbow trout: Discovery of the novel ERalpha2 and both ERbeta isoforms. Gene 392:164-173. https://doi.org/10.1016/j.gene.2006.12.030
  17. Nagler JJ, Cavileer TD, Verducci JS, Schultz IR, Hook SE, Hayton WL (2012) Estrogen receptor mRNA expression patterns in the liver and ovary of female rainbow trout over a complete reproductive cycle. Gen Comp Endocrinol 178:556-561. https://doi.org/10.1016/j.ygcen.2012.06.010
  18. Nelson ER, Habibi HR (2013) Estrogen receptor function and regulation in fish and other vertebrates. Gen Comp Endocrinol 192:15-24. https://doi.org/10.1016/j.ygcen.2013.03.032
  19. Ogawa S, Inoue S, Watanabe T, Hiroi H, Orimo A, Hosoi T, Ouchi Y, Muramatsu M (1998) The complete primary structure of human estrogen receptor beta (hER beta)and its heterodimerization with ER alpha in vivo and in vitro. Biochem Biophys Res Commun 243:122-126. https://doi.org/10.1006/bbrc.1997.7893
  20. Rasier G, Toppari J, Parent AS, Bourguignon JP (2006) Female sexual maturation and reproduction after prepubertal exposure to estrogens and endocrine disrupting chemicals: A review of rodent and human data. Mol Cell Endocrinol 254-255:187-201. https://doi.org/10.1016/j.mce.2006.04.002
  21. Sabo-Attwood T, Kroll KJ, Denslow ND (2004) Differential expression of largemouth bass (Micropterus salmoides) estrogen receptor isotypes alpha, beta, and gamma by estradiol. Mol Cell Endocrinol 218:107-118. https://doi.org/10.1016/j.mce.2003.12.007
  22. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3:1101-1108. https://doi.org/10.1038/nprot.2008.73
  23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725-2729. https://doi.org/10.1093/molbev/mst197
  24. Thomas C, Gustafsson JA (2011) The different roles of ER subtypes in cancer biology and therapy. Nat Rev Cancer 11:597-608. https://doi.org/10.1038/nrc3093
  25. Webb P, Valentine C, Nguyen P, Price RH, Marimuthu A, West BL, Baxter JD, Kushner PJ (2003) ERbeta binds N-CoR in the presence of estrogens via an LXXLL-like motif in the N-CoR C-terminus. Nucl Recept 1:4. https://doi.org/10.1186/1478-1336-1-4
  26. Zavacki AM, Lehmann JM, Seol W, Willson TM, Kliewer SA, Moore DD (1997) Activation of the orphan receptor RIP14 by retinoids. Proc Natl Acad Sci USA 94:7909-7914. https://doi.org/10.1073/pnas.94.15.7909
  27. Zhao C, Dahlman-Wright K, Gustafsson JA (2008) Estrogen receptor beta: An overview and update. Nucl Recept Signal 6:e003.