Journal of Marine Life Science (한국해양생명과학회지)

The Korean Society of Marine Life Science (한국해양생명과학회)
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Induced Ovulation by Hormone Treatments in Red Spotted Grouper, Epinephelus akaara

호르몬 처리에 의한 붉바리 Epinephelus akaara의 배란유도

  • Park, Jong-Youn (Department of Fisheries Science, Chonnam National University) ;
  • Cho, Jae-Kwon (South East Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Park, Chung-Kug (Jeonnam Maritime and Fisheries Science Museum) ;
  • Han, Kyeong-Ho (Department of Fisheries Science, Chonnam National University) ;
  • Hong, Chang-Gi (South Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 박종연 (전남대학교 수산과학과) ;
  • 조재권 (국립수산과학원 남동해수산연구소) ;
  • 박충국 (전라남도 해양수산과학원 해양수산과학관) ;
  • 한경호 (전남대학교 수산과학과) ;
  • 홍창기 (국립수산과학원 남해수산연구소)
  • Received : 2019.12.03
  • Accepted : 2019.12.09
  • Published : 2019.12.13
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Abstract

This study aims to investigate how ovulation induction of red spotted grouper, Epinephelus akaara varies when treated with different hormonal substances such as ovaprim, pimozide, LHRHa, and HCG. As for LHRHa, we injected it at four different concentrations: 50 ㎍kg-1, 100 ㎍kg-1, 150 ㎍kg-1, and 200 ㎍kg-1. All hormonal substances including LHRHa were injected into the back muscles. Buoyant, embryonic survival, fertilization, and hatching rate were the highest in LHRHa and LHRHa + pimozide group. When LHRHa was injected at different concentrations, buoyancy and fertilization rate were the highest in 100 ㎍kg-1 group. Embryonic survival and hatching rate were the highest in 150 ㎍kg-1. Based on these experimental results, we concluded that LHRHa at the concentration of 100 ㎍kg-1 is the most efficient for the ovulation induction in this fish species.

본 연구는 붉바리 배란유도를 위해 다양한 호르몬과(ovaprim, pimozide, LHRHa, HCG)과 LHRHa의 농도별 효과를 조사하였다. LHRHa는 50 ㎍kg-1, 100 ㎍kg-1, 150 ㎍kg-1, 200 ㎍kg-1의 농도로 처리하였다. 호르몬은 등 근육에 주사하였으며, 조사 결과 LHRHa 단독, 그리고 LHRHa와 Pimozide를 혼합하여 투여했을 때 가장 효과적이었으며, 두 실험구 사이의 차이는 거의 없는 것으로 나타났다. 따라서 LHRHa를 단독으로 사용하는 것이 가장 효율적인 것으로 판단된다. 그리고 LHRHa를 다양한 농도로 처리하여 배란유도 효과를 조사한 결과 100 ㎍kg-1의 농도로 투여했을 때 가장 우수한 것으로 조사되었다.

Keywords

Acknowledgement

이 논문은 2019년도 국립수산과학원 수산시험연구사업(R-2019004)의 지원으로 수행된 연구입니다.

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