Fig. 1. Plaque-forming cell(PFC) assay by splenocytes isolated from immunized ICR mice. The mice were during oral administration treated with D, GC and DGC for 9 weeks and intraperitoneally injected with sheep red blood cells(SRBC). Four days after immunization, PFC per spleen cells was counted under a microscope. Control, no treatment group; D 250, Dendropanax morbifera extract 250 mg/kg; D 500, Dendropanax morbifera extract 500 mg/kg; D 750, Dendropanax morbifera extract 750 mg/kg; GC, General cheonggukjang 400 mg/kg; DGC, General cheonggukjang added with Dendropanax morbifera extract 500 mg/kg. The data are means±SD for each group (n=5). Value significantly different from the control (Duncan's multiple comparison test) at p<0.05
Fig. 2. Comparison of immunoglobulin contents in male ICR mouse during oral administration treated with D, GC and DGC for 9 weeks. Control, no treatment group; D 250, Dendropanax morbifera extract 250 mg/kg; D 500, Dendropanax morbifera extract 500 mg/kg; D 750, Dendropanax morbifera extract 750 mg/kg; GC, General cheonggukjang 400 mg/kg; DGC, GC added with Dendropanax morbifera extract 500 mg/kg. The values are means±SD for each group (n=5). Different letters are statistical significantly as determined by Duncan's multiple test (p<0.05).
Fig. 3. Light microscopic pictures of the spleen tissue male ICR mouse during oral administration treated with D, GC and DGC for 9 weeks. Histologic changes in spleen tissue were observed using H&E staining. A, Control; B, Dendropanax morbifera extract (D) 250 mg/kg; C, Dendropanax morbifera extract 500 mg/kg; D, Dendropanax morbifera extract 750 mg/kg; E, Ggeneral chunggukjang 400 mg/kg (GC); F, Dendropanax morbifera extract 500mg/kg + Ggeneral chunggukjang 400 mg/kg (DGC). Scale bars=20 μm.
Table 1. Organ weight of male ICR mouse fed experimental diets after 9 weeks.
Table 2. Hematological data from white blood cell of male ICR mouse oral administration treated with D, GC and DGC for 9 weeks.
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