Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effect of Dendropanax morbifera extract Addition on Chungkukjang on Immune Response of Mice

황칠 추출물을 첨가한 청국장이 마우스의 면역기능에 미치는 효과

  • 김진솔 (조선대학교 생명과학과) ;
  • 정민주 (동강대학교 보건행정과) ;
  • 정경아 (광주보건대학교 임상병리과) ;
  • 송선영 (광주보건대학교 피부미용과) ;
  • 이현화 (조선대학교 생명과학과)
  • Received : 2019.03.29
  • Accepted : 2019.06.07
  • Published : 2019.06.30
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Abstract

This study is to investigate increase immunomodulatory activity of Chungkukjang added with Dendropanax morbifera extract. There are four groups divided that Control, Dendropanax morbifera extract group, Chungkookjang group and Chungkukjang added to Dendropanax morbifera extract group which of five mouses each group. Their immunological characteristics were compared with either general Chungkukjang or non treated group in the tested male ICR mice. There were no significant difference in body weight and organ weight. In the plaque forming cell assay, Dendropanax morbifera extract 250 and 500 mg/kg groups measured significant increase over the control group. The values of lymphocytes were significantly increased in the Dendropanax morbifera extract 500 mg/kg group campared with the control group. In the general Chungkukjang 400 mg/kg added with Dendropanax morbifera extract 500 mg/kg group, total serum immunoglobulin G concentration was significantly higher than the control group and their spleen tissues observed proliferation of white puls. These results demonstrated that Chungkukjang Added with Dendropanax morbifera extract was provided enhance of immunomodulatory activity and suggest that it can be used as various functional foods, based on foods promote immune system health.

본 연구는 황칠추출물과 청국장의 기능성 강화를 위해 황칠추출물이 첨가된 청국장의 면역 활성 효능을 검증하고자 실시하였다. 황칠추출물 250, 500, 750 mg/kg 투여군, 청국장 400 mg/kg 투여군, 청국장 400 mg/kg에 황칠추출물 500 mg/kg을 첨가한 투여군으로 나누어 9주간 마우스에 경구 투여 한 후 체중 및 장기무게, 항체 생산 세포 수, 혈청 면역글로불린의 농도를 측정하고 지라의 조직을 검사하였다. 황칠추출물과 황칠추출물을 첨가한 청국장의 투여가 수컷 마우스의 체중과 장기무게에 미치는 영향을 조사한 결과 모든 투여군에서 체중과 각 장기의 무게는 유의성 있는 차이를 보이지 않았다. 양적혈구에 대한 항체 생산 세포 수 측정에서는 황칠추출물 250 mg/kg과 500 mg/kg 투여군에서 유의성 있게 증가하였으며, 림프구은 황칠추출물 500 mg/kg 투여군이 대조군보다 증가하였다. 황칠추출물 500 mg/kg을 첨가한 청국장 400 mg/kg 투여군은 대조군에 비교하여 IgG 수치가 증가하였으며, 지라 조직 검사결과 백색수질의 증가가 관찰되었다. 이러한 결과는 황칠추출물의 영향으로 황칠 추출물을 첨가한 청국장이 생체 내 면역기능 활성에 효과가 있음을 나타내며 향후 다양한 기능성식품 개발 가능성과 면역증진 식품 소재로 활용될 수 있음을 시사한다.

Keywords

SHGSCZ_2019_v20n6_430_f0001.png 이미지

Fig. 1. Plaque-forming cell(PFC) assay by splenocytes isolated from immunized ICR mice. The mice were during oral administration treated with D, GC and DGC for 9 weeks and intraperitoneally injected with sheep red blood cells(SRBC). Four days after immunization, PFC per spleen cells was counted under a microscope. Control, no treatment group; D 250, Dendropanax morbifera extract 250 mg/kg; D 500, Dendropanax morbifera extract 500 mg/kg; D 750, Dendropanax morbifera extract 750 mg/kg; GC, General cheonggukjang 400 mg/kg; DGC, General cheonggukjang added with Dendropanax morbifera extract 500 mg/kg. The data are means±SD for each group (n=5). Value significantly different from the control (Duncan's multiple comparison test) at p<0.05

SHGSCZ_2019_v20n6_430_f0002.png 이미지

Fig. 2. Comparison of immunoglobulin contents in male ICR mouse during oral administration treated with D, GC and DGC for 9 weeks. Control, no treatment group; D 250, Dendropanax morbifera extract 250 mg/kg; D 500, Dendropanax morbifera extract 500 mg/kg; D 750, Dendropanax morbifera extract 750 mg/kg; GC, General cheonggukjang 400 mg/kg; DGC, GC added with Dendropanax morbifera extract 500 mg/kg. The values are means±SD for each group (n=5). Different letters are statistical significantly as determined by Duncan's multiple test (p<0.05).

SHGSCZ_2019_v20n6_430_f0003.png 이미지

Fig. 3. Light microscopic pictures of the spleen tissue male ICR mouse during oral administration treated with D, GC and DGC for 9 weeks. Histologic changes in spleen tissue were observed using H&E staining. A, Control; B, Dendropanax morbifera extract (D) 250 mg/kg; C, Dendropanax morbifera extract 500 mg/kg; D, Dendropanax morbifera extract 750 mg/kg; E, Ggeneral chunggukjang 400 mg/kg (GC); F, Dendropanax morbifera extract 500mg/kg + Ggeneral chunggukjang 400 mg/kg (DGC). Scale bars=20 μm.

Table 1. Organ weight of male ICR mouse fed experimental diets after 9 weeks.

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Table 2. Hematological data from white blood cell of male ICR mouse oral administration treated with D, GC and DGC for 9 weeks.

SHGSCZ_2019_v20n6_430_t0002.png 이미지

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