디지털융복합연구 (Journal of Digital Convergence)

  • 제17권7호
  • /
  • Pages.275-284
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  • 2019
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  • 2713-6434(pISSN)
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  • 2713-6442(eISSN)
한국디지털정책학회 (The Society of Digital Policy and Management)
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석곡 에탄올 추출물의 항당뇨 약리기전에 관한 연구

A study on Anti-diabetic Mechanism of Ethanol Extract of Dendrobii Herba

  • 박명지 (원광대학교 한의학전문대학원) ;
  • 이영주 (동방문화대학원 약용작물학부)
  • Park, Myung-ji (Wonkwang University Graduate School of Traditional Chinese Medicine) ;
  • Lee, Yeoung-Ju (Department of Medicinal Crops, Dongbang Culture University)
  • 투고 : 2019.05.28
  • 심사 : 2019.07.20
  • 발행 : 2019.07.28
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초록

난초과의 석곡(石斛; Dendrobii herba) 에탄올 추출물(EED)의 항당뇨 활성 및 항당뇨 기전을 조사하였다. 스트렙토 조 토신에 의해 유도된 당뇨병 마우스에서 EED를 하루 4회 경구 투여하여 공복 혈당, 당화혈색소 수치, 인슐린 수치 및 글루탐산 옥살 아세트산 트랜스 아미나 아제 및 글루탐산 피루브산 트랜스 아미나 아제의 간 기능을 감소시키는 실험연구를 통해 그 약리적 기전을 밝혀내고자 했다. EED는 L6 근육 세포에서 세포 내 포도당 섭취뿐만 아니라 RINm5F 베타 세포에서 포도당에 의한 인슐린 분비를 증가시켰다. 따라서 EED는 인슐린 분비를 증가시킬 뿐만 아니라 세포 당 흡수를 증가시킴으로써 항당뇨병 활성을 나타내는데 커다란 가능성을 보여주었으며, 향후 석곡(石斛; Dendrobii herba) 에탄올 추출물에 대한 약리적 기전에 대한 연구가 더욱 활발하게 이루어져 당뇨병 치료에 큰 공헌을 할 수 있게 되기를 기대해본다.

Antidolary active and anti-sugar mechanisms of the ova family (石斛; Dendrobii herba) ethanol extract (EED) were investigated. The EED was administered orally four times a day in a diabetic mouse induced by strepto Joe Toshin to reveal and reveal its pharmacological miracle through experimental studies that reduce the liver function of empty blood sugar, glythamic oxal acetate levels, insulin levels and glutamic acid trans aminaase and glutamic acid pyruvic acid trans amine. EED increased insulin secretion by glucose in RINm5F beta cells as well as intraperitoneal glucose intakes in L6 muscle cells. Thus, EED has shown great promise in displaying anti-diabetes activity not only by increasing insulin secretion but also by increasing intakes per cell, and hopes that future research on pharmacological mechanisms for quartz (Dendrobii herba) ethanol extract will be more active and contribute greatly to the treatment of diabetes.

키워드

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Fig. 1. Effect of EED at different concentrations( 50-400 μg/ml ) against α-glucosidase.

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Fig. 2. Effect of EED on blood glucose levels during oral sucrose tolerance test in normoglycemic mice.

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Fig. 3. Effect of EED on blood glucose levels during oral glucose tolerance test in normoglycemic mice.

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Fig. 4. Effect of EED on insulin secretion in RINm5F cells.

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Fig. 5. Effect of EED on glucose uptake in L6 myotube cells.

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Fig. 6. Effect of EED on fasting blood glucose levels in STZ-diabetic mice.

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Fig. 7. Effect of EED on glycosylated hemoglobin(HbA1c) levels in STZ-diabetic mice.

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Fig. 8. Effect of EED on serum insulin levels in STZ-diabetic mice.

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Fig. 9. Effect of EED on GOT levels in STZ-diabetic mice.

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Fig. 10. Effect of EED on GPT levels in STZ-diabetic mice.

참고문헌

  1. S. H. Yoo. (2018). How to test diabetes complications. Proceedings of KAIM Autumn Conference 2018, 2018(2), 166-168.
  2. J. Heo. (1994). Donguibogam. Seoul : Yeogang Publishing Co.
  3. H. J. Choi. (2004). The emperor's inner circle rumor (sang). Seoul : Free Books.
  4. C. M. Kim, (1997). The chinese medicines dictionary. Seoul : Jungdam.
  5. Y. G. Kim, G. H. Yang & S. I. Cho. (2005). Anti-oxidative effects of dendrobii herba on toxic agent induced kidney cell injury. Master's thesis. Dongshin University, Naju.
  6. H. J. Han, J. H. Kim, H. B. Cho & G. S. Choi. (2000). Effects of herba dendrobii on the ovariectomized rat model of postmenopausal osteoporosis. The Journal Of Oriental Gynecology, 13(2), 120-135.
  7. S. D. Park et al. (2007). Comparison of immunomodualtory effects of water-extracted adenophorae radix, liriopis tuber, dendrobii herba, polygonati odorati rhizoma and polygonati rhizoma. Korean Journal of Oriental Physiology & Pathology, 21(2), 414-424.
  8. M. HwangBo, S. S. Roh & H. S. Seo. (2010). Effects of dendrobii herba and punica granatum extract on the anti-oxidant, anti-inflammatory, anti-wrinkle and whitening. The Journal of Korean Oriental Ophthalmology & Otorhinolaryngology & Dermatology, 23(3), 11-32.
  9. M. Ohsugi et al. (1999). Active-oxygen scavenging activity of traditional nourishing-tonic herbal medicines and active constituents of rhodiola sacra. Journal of Ethnopharmacology, 67(1), 111-119. DOI : 10.1016/s0378-8741(98)00245-1
  10. M. Y. Yoon et al. (2007). Protective effect of methanolic extracts from dendrobium nobile Lindl. on H2O2-induced neurotoxicity in PC12 cells. Journal of the Korean Society for Applied Biological Chemistry, 50(1), 63-67.
  11. K. J. Lee. (1991). Studies on the effect of panax ginseng(Red ginseng) and dendrobium moniliforme on metabolites of alloxan induced diabetic rats. Master's thesis. Dongguk University, Seoul.
  12. American Diabetes Association. (2018). 2. Classification and diagnosis of diabetes: standards of medical care in diabetes-2018. Diabetes Care, 41(Supplement 1), S13-S27. DOI : 10.2337/dc18-s002
  13. Taylor. R. (2013). Type 2 diabetes: Etiology and reversibility. Diabetes Care, 36(4), 1047-1055. DOI : 10.2337/dc12-1805
  14. J. C. Ra, J. H. Bae, H. G. Park & K. S. Kang. (2003). Studies on a new alimentotherapy for diabetic patients. Journal of the Korean Society of Food Science and Nutrition, 32(4), 614-620. DOI : 10.3746/jkfn.2003.32.4.614
  15. S. Y. Rhee. (2018). Monotherapy in type 2 diabetes mellitus patients 2017: A position statement of the korean diabetes association. The Journal of Korean Diabetes, 19(1), 15-22. DOI : 10.4093/jkd.2018.19.1.15
  16. J. K. Grover, V. Vats & S. S. Rathi. (2000). Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. Journal of Ethnopharmacology, 73(3), 461-470. DOI : 10.1016/s0378-8741(00)00319-6
  17. Herbology department of all-korea oriental medicine colleges. (2004). Herbology. Seoul : Youngrimsa.
  18. J. Keizer & G. Magnus. (1989). ATP-sensitive potassium channel and bursting in the pancreatic beta cell. A theoretical study. Biophysical Journal, 56(2), 229-242. DOI : 10.1016/s0006-3495(89)82669-4
  19. N. Kerru, A. Singh-Pillay, P. Awolade & P. Singh. (2018). Current anti-diabetic agents and their molecular targets: A review. European Journal of Medicinal Chemistry, 152, 436-488. DOI : 10.1016/j.ejmech.2018.04.061
  20. S. Seino. (2012). Cell signalling in insulin secretion: the molecular targets of ATP, cAMP and sulfonylurea. Diabetologia, 55(8), 2096-2108. DOI : 10.1007/s00125-012-2562-9
  21. C. R. Sirtori et al. (1978). Disposition of metformin (N,N-dimethylbiguanide) in man. Clinical Pharmacology & Therapeutics, 24(6), 683-693. DOI : 10.1002/cpt1978246683
  22. X. Han et al. (2017). Metformin ameliorates insulitis in STZ-induced diabetic mice. PeerJ, 5, e3155. DOI : 10.7717/peerj.3155
  23. S. H. Park & G. Y. Kim. (2010). Blood glucose level, insulin content and biochemical variables of complexcity extract from oriental medicinal plants on diabetes rats. The Korean Journal of Food And Nutrition, 23(2), 258-268.
  24. Y.-Y. Xu et al. (2017). Dendrobium nobile Lindl. alkaloids regulate metabolism gene expression in livers of mice. Journal of Pharmacy and Pharmacology, 69(10), 1409-1417. DOI : 10.1111/jphp.12778