Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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A study on Anti-diabetic Mechanism of Ethanol Extract of Dendrobii Herba

석곡 에탄올 추출물의 항당뇨 약리기전에 관한 연구

  • Park, Myung-ji (Wonkwang University Graduate School of Traditional Chinese Medicine) ;
  • Lee, Yeoung-Ju (Department of Medicinal Crops, Dongbang Culture University)
  • 박명지 (원광대학교 한의학전문대학원) ;
  • 이영주 (동방문화대학원 약용작물학부)
  • Received : 2019.05.28
  • Accepted : 2019.07.20
  • Published : 2019.07.28
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Abstract

Antidolary active and anti-sugar mechanisms of the ova family (石斛; Dendrobii herba) ethanol extract (EED) were investigated. The EED was administered orally four times a day in a diabetic mouse induced by strepto Joe Toshin to reveal and reveal its pharmacological miracle through experimental studies that reduce the liver function of empty blood sugar, glythamic oxal acetate levels, insulin levels and glutamic acid trans aminaase and glutamic acid pyruvic acid trans amine. EED increased insulin secretion by glucose in RINm5F beta cells as well as intraperitoneal glucose intakes in L6 muscle cells. Thus, EED has shown great promise in displaying anti-diabetes activity not only by increasing insulin secretion but also by increasing intakes per cell, and hopes that future research on pharmacological mechanisms for quartz (Dendrobii herba) ethanol extract will be more active and contribute greatly to the treatment of diabetes.

난초과의 석곡(石斛; Dendrobii herba) 에탄올 추출물(EED)의 항당뇨 활성 및 항당뇨 기전을 조사하였다. 스트렙토 조 토신에 의해 유도된 당뇨병 마우스에서 EED를 하루 4회 경구 투여하여 공복 혈당, 당화혈색소 수치, 인슐린 수치 및 글루탐산 옥살 아세트산 트랜스 아미나 아제 및 글루탐산 피루브산 트랜스 아미나 아제의 간 기능을 감소시키는 실험연구를 통해 그 약리적 기전을 밝혀내고자 했다. EED는 L6 근육 세포에서 세포 내 포도당 섭취뿐만 아니라 RINm5F 베타 세포에서 포도당에 의한 인슐린 분비를 증가시켰다. 따라서 EED는 인슐린 분비를 증가시킬 뿐만 아니라 세포 당 흡수를 증가시킴으로써 항당뇨병 활성을 나타내는데 커다란 가능성을 보여주었으며, 향후 석곡(石斛; Dendrobii herba) 에탄올 추출물에 대한 약리적 기전에 대한 연구가 더욱 활발하게 이루어져 당뇨병 치료에 큰 공헌을 할 수 있게 되기를 기대해본다.

Keywords

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Fig. 1. Effect of EED at different concentrations( 50-400 μg/ml ) against α-glucosidase.

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Fig. 2. Effect of EED on blood glucose levels during oral sucrose tolerance test in normoglycemic mice.

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Fig. 3. Effect of EED on blood glucose levels during oral glucose tolerance test in normoglycemic mice.

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Fig. 4. Effect of EED on insulin secretion in RINm5F cells.

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Fig. 5. Effect of EED on glucose uptake in L6 myotube cells.

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Fig. 6. Effect of EED on fasting blood glucose levels in STZ-diabetic mice.

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Fig. 7. Effect of EED on glycosylated hemoglobin(HbA1c) levels in STZ-diabetic mice.

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Fig. 8. Effect of EED on serum insulin levels in STZ-diabetic mice.

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Fig. 9. Effect of EED on GOT levels in STZ-diabetic mice.

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Fig. 10. Effect of EED on GPT levels in STZ-diabetic mice.

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