Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Mosquito Prevalence and Flavivirus Infection Rates in Gangwon-do, Republic of Korea

2012~2017년 강원지역에서 채집된 모기의 계절적 발생소장과 플라비바이러스 감염률

  • Chung, Se-Jin (Gangwon Institute of Health and Environment) ;
  • Ko, Seuk-Hyun (Gangwon Institute of Health and Environment) ;
  • Ko, Eun-Mi (Gangwon Institute of Health and Environment) ;
  • Lim, Eun-Joo (Gangwon Institute of Health and Environment) ;
  • Kim, Young-Su (Gangwon Institute of Health and Environment) ;
  • Lee, Wook-Gyo (Division of Vectors and Parasitic Diseases, Korea Centers for Disease Control and Prevention) ;
  • Lee, Dong-Kyu (Department of Health and Environment, Kosin University)
  • 정세진 (강원도보건환경연구원) ;
  • 고석현 (강원도보건환경연구원) ;
  • 고은미 (강원도보건환경연구원) ;
  • 임은주 (강원도보건환경연구원) ;
  • 김영수 (강원도보건환경연구원) ;
  • 이욱교 (질병관리본부 매개체분석과) ;
  • 이동규 (고신대학교 보건환경학부)
  • Received : 2019.01.29
  • Accepted : 2019.03.12
  • Published : 2019.06.01
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Abstract

In total, 654,362 adult mosquitoes were captured using black light traps in Gangwon-do Province of the Republic of Korea from 2012 to 2017. The collected mosquitoes were identified to the species level, placed in pools of up to 50 mosquitoes each, by species and date of collection, and screened for flaviviruses using a reverse transcription-polymerase chain reaction assay. A total of 276,224 adult mosquitoes were grouped in 7,721 pools for virus testing, and 68 flavivirus positive pools (0.9%) were detected. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in single pools collected from Chuncheon (2012, 2017: Culex pipiens, 2,728 and 1,111 mosquitoes, respectively), Hoengseong (2013: Culex orientalis, 19), and Gangneung (2017: C. pipiens, 724). All the Japanese encephalitis viruses detected were revealed as genotype V. Chaoyang viruses were detected in 63 pools of 5,055 Aedes vexans nipponii and a single pool of 585 C. pipiens collected in Gangwon-do Province from 2012 to 2017. Chuncheon was the region with the highest minimum infection rates (MIR, 0.32) and maximum likehood estimate (MLE, 0.33; confidence interval (CI) 95%, 0.23-0.46) of A. vexans nipponii for Chaoyang virus, followed by Hoengseong (MIR 0.30, MLE 0.30, CI 0.16-0.52) and Gangneung (MIR 0.21, MLE 0.21, CI 0.13-0.31). Monthly MIR and MLE values of A. vexans nipponii for Chaoyang virus were the highest in October (MIR 0.38, MLE 0.38, CI 0.07-1.25).

2012~2017년까지 강원도 3개 시 군(춘천시, 강릉시, 횡성군)에서 채집된 모기는 총 6속 13종, 654,362마리가 채집되었다. 채집된 모기는 분류하여 얼룩날개모기속을 제외하고 종별, 채집일, 채집 장소에 따라 최대 50마리를 1개 실험군으로 reverse transcription-polymerase chain reaction, 염기서열 분석방법으로 플라비바이러스 감염여부를 조사하였다. 채집모기 276,224마리에 대해 7,721개 실험군을 검사한 결과 68개 실험군(0.9%)에서 플라비바이러스 유전자가 검출되었다. 검출된 플라비바이러스의 염기서열 분석결과 4개 실험군은 일본뇌염바이러스(Japanese encephalitis virus), 64개 실험군은 차오양바이러스(Chaoyang virus)로 확인되었다. 일본뇌염바이러스는 2012년 춘천시에서 채집된 2,728마리의 빨간집모기 중 1개 실험군, 2013년 횡성군에서 채집된 19마리의 동양집모기 중 1개 실험군, 2017년 춘천시에서 채집된 1,111마리의 빨간집모기 중 1개 실험군, 강릉시에서 채집된 빨간집모기 724마리 중 1개 실험군에서 검출되었다. 검출된 일본뇌염바이러스의 유전형은 모두 V형 바이러스였다. 차오양바이러스는 강원도 내에서 6년간 채집된 금빛숲모기 232,871마리, 5,055개 실험군을 대상으로 검사한 결과 63개 실험군에서 검출되었으며, 춘천지역에서 채집된 빨간집모기 585개 실험군 중 1개 실험군에서 검출되었다. 채집지역별 금빛숲모기의 차오양바이러스 감염률이 가장 높은 지역은 MIR (최소감염률) 0.32, MLE (최대우도법) 0.33 (CI 0.23~0.46) 감염률을 보인 춘천시였다. 그 뒤로 횡성군 MIR 0.30, MLE 0.30 (CI 0.16~0.52)과 강릉시 MIR 0.21, MLE 0.21 (CI 0.13~0.31)순이었다. 월별 금빛숲모기의 차오양바이러스 감염률은 10월에 MIR 0.38, MLE 0.38 (CI 0.07~1.25)로 가장 높은 감염률을 나타내었다.

Keywords

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Fig. 1. Distribution of female mosquitoes collected weekly at the three study sites, from 2012 to 2017.

Table 1. Real time RT-PCR conditions and melt curve program for the detection of flaviviruses

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Table 2. Summary of trap index (TI) for mosquitoes collected by light traps at three study sites in Gangwon-do, the Republic of Korea, from 2012 to 2017

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Table 3. Total number of specimens and poolsa for flavivirus detection in Gangwon-do, the Republic of Korea, from 2012 to 2017

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Table 4. Summary of Japanese encephalitis virus infection rates using MIR and MLE values for three regions in Gangwon-do, the Republic of Korea, from 2012 to 2017

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Table 5. Summary of Chaoyang virus infection rates using MIR and MLE values for three regions in Gangwon-do, the Republic of Korea, from 2012 to 2017

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Table 6. Collection sites and dates, and mosquito species for flavivirus-positive mosquito pools, from 2012 to 2017

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Table 6. Continued

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Table 7. Summary of MIR and MLE of Chaoyang virus infection rates by month in Gangwon-do, the Republic of Korea, from 2012 to 2017

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