Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Penicillium italicum-Specific Primers for Rapid Detection among Fungal Isolates in Citrus

  • Chen, Kai (School of Bioengineering and Food, Hubei University of Technology) ;
  • Tian, Zhonghuan (Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University) ;
  • Jiang, Fatang (School of Bioengineering and Food, Hubei University of Technology) ;
  • Long, Chao-an (Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University)
  • Received : 2019.04.12
  • Accepted : 2019.05.08
  • Published : 2019.06.28
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Abstract

Blue mold in citrus is caused by Penicillium italicum. In this study, the P. italicum-specific primers were developed for rapid detection based on the conserved genes RPB1 and RPB2 among Penicillium genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detect P. italicum. The PCR assay among 39 fungal isolates and the colonial, pathogenic morphologies and molecular methods validated the specificity and reliability of these two primer pairs. This report provided a method and P. italicum-specific primers, which might greatly contribute to citrus postharvest industry.

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