Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of LED light on the inactivation of Bacillus cereus for extending shelf-life of extruded rice cake and simulation of the patterns of LED irradiation by various arrays of LEDs

압출떡의 유통기한 연장을 위한 LED 조사의 Bacillus cereus 억제 효과 및 LED의 배열에 따른 빛의 조사 패턴 시뮬레이션

  • Jung, Hwabin (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Yuk, Hyun-Gyun (Department of Food Science and Technology, Korea National University of Transportation) ;
  • Yoon, Won Byong (Department of Food Science and Biotechnology, Kangwon National University)
  • Received : 2019.03.21
  • Accepted : 2019.05.14
  • Published : 2019.06.30
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Abstract

The optimum design of LED device for irradiation of 460 nm blue light on extruded rice cake using simulation and the effect of the blue light on the inactivation of Bacillus cereus (B. cereus) group on the rice cake were investigated. The irradiated light intensity patterns on the surface area of the sample were simulated with three different LED arrays (centered, cross, and evenly spaced) and at various distances (22, 32, 42 mm) between the LED modules and the sample. In addition, the uniformity was calculated as Petri factor. The evenly spaced array resulted the most uniform light intensity pattern in the simulation, and the Petri factor of 32 and 42 mm of the distances showed higher than 0.9, which represents the ideal uniformity of LED device. The bacterial population of the rice cake decreased to less than the initial bacterial population during exposure to LED blue light, whereas the bacterial population of the control sample increased. The bacterial count of the rice cake after blue light irradiation for 24 h was 1.21 log CFU/g lower than the control sample. Petri factor increased with increase of the distance between the light source and sample, however, the reduction rate of B. cereus group decreased. Therefore, the design of LED device, that represented the Petri factor higher than 0.9 and inactivated the population of B. cereus group, with evenly spaced and 32 mm of distance between the light source and sample was suitable for extending shelf-life of rice cake.

본 연구에서는 460 nm 파장의 청색 가시광선을 압출 떡의 표면에 조사하기 위하여 장치의 최적 디자인을 시뮬레이션을 통하여 확인하고 청색광이 압출 떡의 표면에서 식품 위해균인 B. cereus group에 미치는 영향을 확인하였다. LED 장치에서 광원모듈의 세 가지 배열(centered, cross, evenly spaced) 및 광원과 샘플 표면 사이의 거리(22, 32, 42 mm)에 따른 조사 면적에서 빛의 세기 패턴을 시뮬레이션을 통하여 계산하고, Petri factor를 통하여 균일도를 확인하였다. LED 배열의 균일도는 evenly spaced 배열에서 가장 균일한 패턴을 보였으며, 광원과의 거리가 32 및 42 mm일 경우 Petri factor가 0.9 이상으로 높은 균일도를 나타내었다. 떡볶이 떡에 LED 청색광을 조사한 경우 24 h 후 균 수가 초기 균 수에 비하여 감소하였으며, LED를 조사하지 않은 대조군에서는 초기 균 수가 증가하여 1.21 log CFU/g의 차이를 보였다. LED 조사 시 광원과 샘플의 거리가 증가할수록 Petri factor는 증가하나 감균 효과가 낮아지는 결과를 나타내었다. 따라서 Petri factor가 0.9 이상임을 만족하는 evenly spaced 배열의 32 mm 거리가 떡의 유통기한 연장을 위한 LED 장치의 디자인에 적합함을 확인하였다.

Keywords

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