Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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Effects of Aerobic Exercise and Chrysin Supplementation on Macrophage Infiltration and Lipolysis Genes of High-Fat Diet Mice

고지방식이 동물모델에서 크리신 섭취와 유산소 운동이 대식세포 침윤과 지방분해 유전자들에 미치는 영향

  • Received : 2019.03.25
  • Accepted : 2019.05.20
  • Published : 2019.05.28
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Abstract

This study was investigated the effect of aerobic exercise and chrysin supplementation on macrophage infiltration and lipolysis in high-fat diet mice. To accomplish the purpose of this study, C57BL/6 mice were fed high fat diet(60% fat diet) during experimental period. The animals were divided into 4 groups; NC (normal diet control, n=5), HC (high fat diet control, n=5), Hch(high fat diet with chrysin, n=5), and HME (high fat diet with aerobic exercise training, n=5). Exercise training was performed for 16 weeks on a treadmill running. As a result, macrophage marker, F480 and CD11c were significantly decreased in HME comparison with HD and Hch. Also, M2 macrophage marker CD11c, and lipolysis marker PRDM were significantly increased in HME compared with HC and Hch These findings suggest that regular aerobic exercise has beneficial effects to inhibit macrophage infiltration in high fat diet mice.

본 연구 목적은 유산소운동과 크리신섭취가 고지방식이동물의 간 조직에서 비만억제 효과를 규명하는데 있다. 집단은 정상식이, 고지방식이, 고지방식이와 크리신섭취, 고지방식이와 유산소운동 4집단으로 하였다. 크리신은 체중당 50mg/kg을 구강투여 하였고, 유산소운동은 트레드밀운동으로 주5회 60분 16주간 실시하였다. SPSS(20.0)프로그램을 이용해 일원변량분석(One-way ANOVA)을 하였고, 사후분석은 LSD로 하였다. 연구결과 간 조직에서 대식세포 마커 F480와 M1대식세포 마커 CD11c는 정상식이 그룹과 비교해 고지방식이, 크리신투여 그룹에서 유의하게 증가했고 운동집단에서는 유의하게 감소하였다. 지방분해 마커 PRDM은 정상식이집단과 비교해 고지방식이, 크리신투여 집단에서 유의하게 감소했으나 운동집단에서만 유의하게 증가하였다. 결론적으로 고지방식이와 중강도 운동은 간 조직에서 발생되는 대사적불균형을 억제하는데 효과적인 것으로 나타났다. 하지만 고지방식이와 크리신 섭취는 비만억제 기능이 미비한 것으로 나타났다. 따라서 향후 기능성식품을 이용한 비만개선 연구는 투여용량, 기간 등을 고려해 다양한 분자적 기전을 살펴보는 연구가 보강되어야 할 것이다.

Keywords

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Fig. 1. Effect of aerobic exercise and chrysin supplementation on macrophage marker in liver (A) F480 mRNA (B) Representative microphotograph of hemat oxylin and eosin(H&E) staining from mice on each of the four groups.

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Fig. 2. Effect of aerobic exercise and chrysin supplementation on M1, M2 marker in liver.

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Fig. 3. Effect of aerobic exercise and chrysin supplementation on lipolysis marker in liver.

Table 1. Primer sequences used for RT-PCR

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Table 2. Change of F480 mRNA

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Table 3. Change of CD11c, CD206 mRNA

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Table 4. Change of PRDM, PPAR-α mRNA

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