The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Validation of Simultaneous Analysis Method of Standard Compounds in Fermented Kalopanax pictus Nakai by Bioconversion

생물전환을 통한 음나무발효물의 지표성분 설정 및 동시분석법 검증

  • 장원희 ((주)에스티알바이오텍 중앙연구소) ;
  • 이화영 ((주)에스티알바이오텍 중앙연구소) ;
  • 이봉진 ((주)에스티알바이오텍 중앙연구소) ;
  • 김진만 ((주)에스티알바이오텍 중앙연구소) ;
  • 박선주 ((주)에스티알바이오텍 중앙연구소)
  • Received : 2019.03.08
  • Accepted : 2019.03.18
  • Published : 2019.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to select compounds for the standardization of fermented Kalopanax pictus Nakai (KP-F), to develop the analysis method using HPLC-PDA and to perform method validation. KP-F is a fermented powder developed to improve the original physiological activities and create a new functionality. Eleutheroside E, Acanthoside B, and Syringaresinol were selected as the standard compounds and developed our own method for simultaneous analysis. The analyte was isolated using C18 column with a gradient elution of 0.05 M phosphoric acid in water and methanol as the mobile phase at a flow rate of 1 mL/min and detected at 210 nm. As a result, all standard compounds showed good linearity with an $R^2$ (coefficient of correlation) of 1.000 and for the limit of detection range of $0.710{\sim}0.831{\mu}g/mL$, and the limit of quantification as $2.150{\sim}2.520{\mu}g/mL$. The precision was RSD (%) of less than 4.80%, while the accuracy was 4.70%>RSD (%) for the range 102.44~110.48%. In conclusion, the developed analysis method is suitable for the detection of Eleutheroside E, Acanthoside B, and Syringaresinol in KP-F.

Keywords

HGSPB1_2019_v32n2_148_f0001.png 이미지

Fig. 2. Chromatogram of (A) standards mixture of Eleutheroside E, Acanthoside B, and Syringaresinol, (B) Kalopanax pictus Nakai (KP), (C) Fermented Kalopanax pictus (KP-F).

HGSPB1_2019_v32n2_148_f0002.png 이미지

Fig. 1. Chromatogram of (A) standards mixture of Kalopanaxsaponin B and Kalopanaxsaponin A, (B) Kalopanax pictus Nakai(KP), (C) Fermented Kalopanax pictus Nakai (KP-F).

HGSPB1_2019_v32n2_148_f0003.png 이미지

Fig. 3. Compound structures of Kalopanax pictus Nakai(KP) converted through Bioconversion.

HGSPB1_2019_v32n2_148_f0004.png 이미지

Fig. 4. Content graph of (A-1) Kalopanaxsaponin B and Kalopanaxsaponin A in Kalopanax pictus Nakai (KP), (A-2) Kalopanaxsaponin B and Kalopanaxsaponin A in fermented Kalopanax pictus Nakai (KP-F), (B-1) Eelutheroside E, Acanthoside B, and Syriangaresinol in Kalopanax pictus Nakai (KP), (B-2) Eelutheroside E, Acanthoside B, and Syriangaresinol in fermented Kalopanax pictus Nakai (KP-F).

HGSPB1_2019_v32n2_148_f0005.png 이미지

Fig. 5. UV spectrum of (A) Eleutheroside E, (B) Acanthoside B, and (C) Syringaresinol.

HGSPB1_2019_v32n2_148_f0006.png 이미지

Fig. 6. Chromatogram of (A) Blank (70% EtOH), (B) Standards mixture of Eleutheroside E, Acanthoside B, and Syringaresinol, (C) Fermented Kalopanax pictus (KP-F).

Table 1. Content of Kalopanaxsaponins and Liganan glycosides in fermented Kalopanax pictus Nakai (KP-F)

HGSPB1_2019_v32n2_148_t0001.png 이미지

Table 2. Calibration curves, detection limits (LOD) and quantification limits (LOQ) of Eleutheroside E, Acanthoside B, and Syringaresinol

HGSPB1_2019_v32n2_148_t0002.png 이미지

Table 3. Precision of fermented Kalopanax pictus Nakai (KP-F)

HGSPB1_2019_v32n2_148_t0003.png 이미지

Table 4. Accuracy of Eleutheroside E, Acanthoside B, and Syringaresinol

HGSPB1_2019_v32n2_148_t0004.png 이미지

References

  1. Cho YH, Cho JS, Lee GW. 2011. Antioxidant activity of wood vinegar by bioconversion. J Korea Academia-Industrial Coop Soc 12:4434-4442 https://doi.org/10.5762/KAIS.2011.12.10.4434
  2. Choi J, Huh K, Kim SH, Lee KT, Lee HK, Park HJ. 2002. Kalopanaxsaponin A from Kalopanax pictus, a potent antioxidant in the rheumatoidal rat treated with Freund's complete adjuvant reagent. J Ethnopharmacol 79:113-118 https://doi.org/10.1016/S0378-8741(01)00382-8
  3. Jo K, Han SH, Bae SH, Shin JC, Suh HJ. 2018. HPLC validation of valerian ethanol extract as a functional food. Korean J Food Nutr 31:104-108 https://doi.org/10.9799/ksfan.2018.31.1.104
  4. Kim DW, Bang KH, Rhee YH, Lee KT, Park HJ. 1998. Growth inhibitory activities of kalopanaxsaponins A and I against human pathogenic fungi. Arch Pharm Res 21:688-691 https://doi.org/10.1007/BF02976758
  5. Ko HR, Ahn SC. 2007. Microbial mimic metabolism of natural products. J Mar Biosci Biotechnol 2:11-22
  6. Lee JH, Moon SY, Cho BY, Choi SI, Jung TD, Choi SH, Kim JD, Lee OH. 2017. Stability of ethanolic extract from fermented Cirsium setidens Nakai by bioconversion during different storing conditions. Korean J Food Nutr 30:388-394 https://doi.org/10.9799/ksfan.2017.30.2.388
  7. Lee MW, Hwang WK, Hahn DR. 1989. Phytochemical studies on the constituents of the bark of Kalopanax pictum var. maximowiczii. Chung-Ang J Pharm Sci 3:13-19
  8. Li DW, Lee EB, Kang SS, Hyun JE, Whang WK. 2002. Activity-guided isolation of saponins from Kalopanax pictus with anti-inflammatory activity. Chem Pharm Bull 50:900-903 https://doi.org/10.1248/cpb.50.900
  9. Park HJ, Kwon SH, Lee JH, Lee KH, Miyamoto Ki, Lee KT. 2001. Kalopanaxsaponin A is a basic saponin structure for the anti-tumor activity of Hederagenin monodesmosides. Plant Med 67:118-121 https://doi.org/10.1055/s-2001-11516
  10. Quang TH, Ngan NTT, Minh CV, Kiem PV, Nhiem NX, Tai BH, Thao NP, Luyen BTT, Song SB, Kim YH. 2011. Anti-inflammatory and PPAR subtypes transactivational activities of phenolics and lignans from the stem bark of Kalopanax pictus. Bull Korean Chem Sor 32:4049-4054 https://doi.org/10.5012/bkcs.2011.32.11.4049
  11. Sano K, Sanada S, Ida Y Shoji J. 1991. Studies on the constituents of the bark of Kalopanax pictus Nakai. Chem Pharm Bull 39:865-870 https://doi.org/10.1248/cpb.39.865
  12. Shao CJ, Kasai R, Xu JD, Tanaka O. 1989. Saponins from roots of Kalopanax septemlobus (Thunb.) Koidz., Ciqiu: Structures of Kalopanax-saponins C, D, E and F. Chem Pharm Bull 37:311-314 https://doi.org/10.1248/cpb.37.311
  13. Sun WJ, Zhang DK, Sha ZF, Zhang HL, Zhang XL. 1990. Studies on the saponin constituents of Kalopanax septemlobus (Thunb.) Koidz. Yao Xue Xue Bao 25:29-34