Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Variation in Fatty Acid Composition, Caffeic and Rosmarinic Acid Content, and Antioxidant Activity of Perilla Accessions

들깨 유전자원의 지방산 변이 및 rosmarinic acid와 caffeic acid의 함량에 따른 항산화 활성 비교

  • 김다정 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • ;
  • 정이진 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 전영아 (농촌진흥청 국제기술협력과) ;
  • 이재은 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이명철 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이호선 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이주희 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 성정숙 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Received : 2019.03.25
  • Accepted : 2019.04.12
  • Published : 2019.04.30
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Abstract

Background: The aim of this study was to compare the fatty acid composition, antioxidant activity, and the content of two major phenolic acids, caffeic and rosmarinic acids, of 45 Perilla accessions collected from Russia. Methods and Results: A total of 45 accessions of the genus Perilla were used in this study. The antioxidant activities of these accessions were analyzed using a spectrophotometer, and their caffeic and rosmarinic acid contents were determined using a reversed-phase ultraperformance liquid chromatography (UPLC) system. The Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using a Shimadzu QP2010 gas chromatography-mass spectoroscopy system. The results showed that accessions IT226732 and IT274300 had the highest content of caffeic and rosmarinic acid, respectively. A cluster analysis based on antioxidant assay results and concentration of phenolic acids led to the categorization of Perilla accessions into three major groups. The accessions in Group I were characterized by higher caffeic and rosmarinic acid content and antioxidant activity, compared to the accessions in the other two groups (p < 0.05). The total oil content ranged between 28.39 and 46.89%. The compositions of oleic, linoleic, and linolenic acid ranged from 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Conclusions: The results of this study indicated that accessions IT274300, IT226732, IT274293, IT235818, and IT235820 could be used as a source of functional materials.

Keywords

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Fig. 1. Peak of caffeic acid was detected at 3.2 min (a) and a peak of rosmarinic acid was found at 11.3 min (b) by UHPLC system.

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Fig. 2. Hierarchical clustering analysis of antioxidant activities in perilla 45 accessions.

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Fig. 3. Relative antioxidant capacity index (RACI) of perilla 48 accessions.

Table 1. Conditions of UPLC for identification and determination of caffeic acid and rosmarinic acid.

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Table 3. Total oil and fatty acid contents of seed extracts from 45 perilla accessions.

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Table 3. Continued.

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Table 4. Eigenvector and eigenvalues generated by principal component analysis of perilla 45 accessions.

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Table 5. Pearson correlation coefficients between antioxidant activities and major phenolic phytochemicals.

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Table 6. Average values of each groups based on antioxidant activities and major phenolic phytochemicals.

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Table 7. Eigenvector and eigenvalues generated by Principal Component Analysis of fatty acid composition.

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Table 8. Pearson correlation coefficients for oil composition ofperilla.

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Table 9. Average values of each groups based on fatty acidcomposition.

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Table 2. Caffeic, rosmarinic acid contents and antioxidant activities of leaf extracts from 45 perilla accessions.

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Table 2. Continued.

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