Fig. 2. Phylogenetic tree showing position of the biocontrol agents (▲) used in this study. The 16S rDNA sequences were aligned with the ClustalW and the tree was constructed with the maximum likelihood method based on the Tamura-Nei model integrated in the MEGA7 software. The GenBank accession numbers of the DNA sequences are shown in parentheses.
Fig. 4. The effect of bacterization of tomato seeds with two biocontrol agents on root length of tomato seedlings. Data are mean of three replications. Bars indicate error of the mean.
Fig. 5. The effect of bacterization of tomato seeds with two biocontrol agents on the fresh weight of tomato seedlings. Data are mean of three replications. Bars indicate standard error of the mean.
Fig. 1. Inhibition of F. oxysporum f. sp. lycopersici by two bacterial isolates (A) Bacillus methylotrophicus Prb1, and (B) Brevundimonas olei Prd2, and (C) mycelial growth of F. oxysporum in control plate.
Fig. 3. The effect of bacterization of tomato seeds with two biocontrol agents on shoot length of tomato seedlings. Data are mean of three replications. Bars indicate standard error of the mean.
Fig. 6. The effect of bacterization of tomato seeds with two biocontrol agents on vigor index of tomato seedlings. Data are mean of three replications. Bars indicate standard error of the mean.
Table 1. Location of the agricultural fields for the collection of rhizospheric soil samples
Table 4. Survival of selected bacterial isolates at an interval of 7 days
Table 2. In vitro biocontrol activity of bacterial isolates against Fusarium oxysporum
Table 3. Biochemical and physiological characteristics of two biocontrol agents
Table 5. Effect of concentration of ferric chloride on the mycelial growth (mm) of Fusarium oxysporum
Table 6. Effect of bacterial isolates on the seed germination of two cultivars (Pusa Ruby and Ratan) of tomato
Table 7. The average disease incidence and biocontrol efficacy in controlling Fusarium wilt of treated tomato plant with rhizobacterial isolates in year 2015-16 and 2016-17
Table 8. Effect of bacterial isolates on growth parameters of tomato (cv. Pusa Ruby)
Table 9. Effect of bacterial isolates on growth parameters of tomato (cv. Ratan)
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