Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic diversity of grapevine (Vitis vinifera L.) as revealed by ISSR markers

  • Basheer-Salimia, Rezq (Department of Plant Production and Protection, College of Agriculture, Hebron University) ;
  • Mujahed, Arwa (Department of Applied Biology, College of Applied Science, Palestine Polytechnic University)
  • Received : 2019.02.01
  • Accepted : 2019.02.15
  • Published : 2019.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The main goal of this study was to determine the genetic diversity among 36 grape cultivars grown in Palestine by using ISSR-polymerase chain reaction (PCR) fingerprints. Among the tested primers, 17 produced reasonable amplification products with high intensity and pattern stability. A total of 57 DNA fragments (loci) separated by electrophoresis on agarose gels were detected and they ranged in size, from 150 to 900 bp. Out of these fragments, 55 (88%) were polymorphic and 2 (3.5%) monomorphic. Our results also revealed an average of 3.1 loci per primer. A minimum of 1 and maximum of 10 DNA fragments were obtained (S-17, #820 and #841) and (S-31) primers, respectively. Therefore, the later primer (S-31) is considered to be the most powerful primer among the tested ones. The genetic distance matrix showed an average distance range of between 0.05 and 0.76. The maximum genetic distance value of 0.76 (24% similarity) was exhibited between the (Shami and Marawi.Hamadani.Adi) as well as (Bairuti and Marawi.Hamadani.Adi) genotypes. On the other hand, the lowest genetic distance of 0.05 (95% similarity) was exhibited between (Jandali.Tawel.Mofarad and Jandali. Kurawi.Mlzlz) along with (Shami.Aswad and Shami.mtartash. mlwn) genotypes. Furthermore, the UPGMA dendrogram generally clusters the grape cultivars into eight major clusters in addition to an isolated genotype. Based on these figures, the cultivars tested in this study could be characterized by large divergence at the DNA level. This is taking the assumption that our region has a very rich and varied clonal grape genetic structure.

Keywords

References

  1. Achtak H, Oukabli A, Ater M, Santoni S, Kjellberg F, Khadari B (2009) Microsatellite Markers as Reliable Tools for Fig Cultivar Identification. J Amer Soc Hort. Sci 134(6):624-631 https://doi.org/10.21273/JASHS.134.6.624
  2. Agarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports 27:617-631 https://doi.org/10.1007/s00299-008-0507-z
  3. Bahurupe J, Sakhare S, Kulwal P, Akhare A, Pawar B (2013) Genetic diversity analysis in chilli (Capsicum annuum L.) using RAPD markers. The international quarterly journal of life sciences 8(3):915-918
  4. Basheer-Salimia R, Awad M, Ward J (2012). Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.). American Journal of Plant Sciences 3(9):1241-1251 https://doi.org/10.4236/ajps.2012.39150
  5. Basheer-Salimia R (2015a) Ampelographic and phenotypic characterizations of white Palestinian grapevine cultivars. Palestine Technical University-Kadoorie Research Journal 3(1):1-11
  6. Basheer-Salimia R (2015b) Identification of Palestinian coloredtable-grape cultivars by means of morphological and pomological descriptors. American Journal of Experimental Agriculture 9(5):1-15 https://doi.org/10.9734/AJEA/2015/19468
  7. Basheer-Salimia R, Ward J (2014) Climate change and its effects on olive tree physiology in Palestine. Review of Research Journal 3(7):1-7
  8. Casasoli M, Mattioni C, Cherubini M, Villani F (2001) Genetic linkage map of European chestnut (Castenea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theor Appl Genet 102:1190-1199 https://doi.org/10.1007/s00122-001-0553-1
  9. Chittora M, Sharma D, Veer C, Verma G (2015) Molecular markers: An important tool to assess genetic fidelity in tissue culture grown long-term cultures of economically important fruit plants. Asian Journal of Bio Science 10(1):101-105 https://doi.org/10.15740/HAS/AJBS/10.1/101-105
  10. Choudhary RS, Zagade_Maboodurrahman VS, Khalakar GD, Singh NK (2014) ISSR based genotypic differentiation of grape (Vitis vinifera L.) Bioscan 9(2):823-828
  11. Depnath S (2009) Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium. Nordic Journal of Botany 27:141-148 https://doi.org/10.1111/j.1756-1051.2009.00402.x
  12. Dhanorkar VM, Tamhankar SA, Patil SG, Rao VS (2005) ISSR-PCR for assessment of genetic relationships among grape varieties cultivated in India. Vitis 44 (3):127-131
  13. Herrera R, Cares V, Wilkinson MJ, Caligari PD (2002) Characterization of genetic variation between (Vitis vinifera) cultivars from central Chile using RAPD and inter simple sequence repeat markers. Euphytica 124:139-145 https://doi.org/10.1023/A:1015693721532
  14. Jing Z, Wang X, Cheng J (2013) Analysis of genetic diversity among Chinese wild Vitis species revealed with SSR and SRAP markers. Genet Mol Res 12(2):1962-1973 https://doi.org/10.4238/2013.June.13.5
  15. Lima ML, Garcia AA, Oliveira KM, Matsuoka S, Arizono H, De Souza-CL Jr, De-Souza AP (2002) Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar cane (Saccharum spp.). Theor Appl Genet. 104(1): 8-30
  16. MOA (2017) Strategy for grape sector in Palestine "2017-2022". Publications of the Ministry of Agriculture, Ramallah, Palestine
  17. Moreno S, Martin JP, Ortiz JM (1998) Inter-simple sequence repeats PCR for characterization of closely related grape vine germplasm. Euphytica 101:117-125 https://doi.org/10.1023/A:1018379805873
  18. Qian W, Ge S, Hong DY (2001) Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR. Theor Appl Genet 102:440-449 https://doi.org/10.1007/s001220051665
  19. Reddy M, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9-17 https://doi.org/10.1023/A:1020691618797
  20. Riaz S, Gabriella De-Lorenzis G, Velasco D, Koehmstedt A, Maghradze D, Bobokashvili Z, Musayev M, Zdunic G, Laucou V, Walker M, Failla O, Preece J, Aradhya M, Arroyo-Garcia R (2018) Genetic diversity analysis of cultivated and wild grapevine (Vitis vinifera L.) accessions around the Mediterranean basin and Central Asia. BMC Plant Biology 18(137):1-14 https://doi.org/10.1186/s12870-017-1213-1
  21. Sabir A, Tangolar S, Buyukalaca S, Kafkas S (2009) Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars. Czech Journal of Genetic and Plant Breeding 45(4):160-168 https://doi.org/10.17221/72/2008-CJGPB
  22. Santos AR, Duarte D, Sousa S, Eiras_Dias J, Gomes AC (2013) An optimized set of microsatellites for grapevine identification. 36th world congress of vine and wine; 2-7 June 2013 Bucharest, Romania (Organisation Internationale de la Vigne et du Vin: Paris, France)
  23. Schluter PM, Harris SA (2006) Analysis of multi-locus fingerprinting data sets containing missing data. Mol Ecol 6:569-572 https://doi.org/10.1111/j.1471-8286.2006.01225.x
  24. Sripholtaen A, Charoenchai C, Urairong H (2016). Application of microsatellite markers for identification of wine grape varieties in Thailand. Asia-Pac J Sci Technol 21(1):97-110
  25. Sultan S (2005). Grapevines, establishing, planting, training, pruning, servicing, p. 459-464 (In Arabic)
  26. Thomas MR, Matsumoto S, Cain P, Scott NS (1993) Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theor Appl Genet 86:73-180
  27. Vignani R, Bowers JE, Meredith CP (1996) Microsatellite DNA polymorphism analysis of clones of Vitis vinifera Sangiovese. Sci Hort 65:163-169 https://doi.org/10.1016/0304-4238(95)00865-9
  28. Wang Z, Weber JL, Zhong G, Tanksley SD (1994) Survey of plant short tandem DNA repeats. Theor Appl Genet 88:1-6 https://doi.org/10.1007/BF00222386
  29. Weising K, Atkinson RG, Gardner RC (1995) Genomic finger printing by microsatellite primed PCR: A critical evaluation. PCR Meth Appl 4:249-255 https://doi.org/10.1101/gr.4.5.249