Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Quality Properties of Herbal Wine containing Schizandra chinensis and Lycium chinense according to Extract Concentration

추출농도에 따른 오미자 및 구기자를 첨가한 한방약술의 품질특성

  • Oh, Sung-Cheon (Dept. of Pharmaceutical Quality Control, Daewon University College)
  • 오성천 (대원대학교 제약품질관리과)
  • Received : 2018.12.02
  • Accepted : 2019.03.29
  • Published : 2019.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, the following is the result of measuring the quality characteristics of herbal wine and the active inhibition of Glutathione S-transferase in order to measure the release of physiological active substances according to the concentration of extracts. The pH level of herbal wine was 4.4, up from 3.9 before fermentation. These changes are attributed to fermentation and organic acids during alcoholic fermentation. The acidity of herbal wine was 0.55%, about six times higher than the pre-fermentation control of 0.09%. These results show that organic acids are used for flavor formation, ether, in combination with alcohol. The inhibitory activity of glutathione S-transferase were $5.1{\pm}0.31$ in herbal wine 15%, $6.5{\pm}0.6$ in herbal wine 20%, $7.6{\pm}0.6$ in herbal wine 25%, $8.4{\pm}0.2$ in herbal wine 30% and $9.7{\pm}0.7$ in herbal wine 35%. As the extract concentration was increased the inhibitory activity of glutathione S-transferase were significantly increased (<0.05).

본 연구에서는 한약재의 활용도를 높이기 위한 연구로 추출물 농도에 따른 생리활성물질의 용출량을 측정하기 위해 한방약술의 품질 특성과 Glutathione S-transferase의 활성 저해능을 측정한 결과는 다음과 같다. 한방약술의 pH결과는 4.4로 발효 전의 대조구 3.9보다 증가하였다. 이러한 변화는 알코올 발효과정 중 발효부산물 및 유기산 때문으로 사료된다. 한방약술의 산도는 0.55%로 발효 전의 대조구 0.09%보다 약 6배 증가하였다. 이런 결과는 유기산이 알코올 등과 결합하여 ester와 같은 향미 형성 등에 이용되는 것을 알 수 있다. 한방약술 15%의 glutathione S-transferase의 활성 저해능 $5.1{\pm}0.3$, 한방약술 20%는 저해능 $6.5{\pm}0.5$, 한방약술 25%는 $7.6{\pm}0.6$, 한방약술 30%는 $8.4{\pm}0.2$, 최대 농도인 35%에서의 저해능은 $9.7{\pm}0.7$로 나타내었다. 추출 농도별로 glutathione S-transferase의 활성 저해능은 통계학적으로 유의한 차이가 있음을 보였다 (p<0.05).

Keywords

Table 1. Measurement method of activity on the substrate

HGOHBI_2019_v36n1_341_t0001.png 이미지

Table 2. Proximate composition of Schizandra chinensis and Lycium chinense(%)

HGOHBI_2019_v36n1_341_t0002.png 이미지

Table 3. pH of Herbal drug Wine

HGOHBI_2019_v36n1_341_t0003.png 이미지

Table 4. Total acidity of Herbal Drug Wine

HGOHBI_2019_v36n1_341_t0004.png 이미지

Table 5. Inhibition ability of herbal drug wine depending on glutathione S-transferase

HGOHBI_2019_v36n1_341_t0005.png 이미지

References

  1. I. S. Choi, K. Y. Kim, J. E. Yim and Y.S. Kim, "Calorie restriction and obesity under the regulation of SIRTI". Korean J. Obes. Vol.20, pp. 170-176, (2011). https://doi.org/10.7570/kjo.2011.20.4.170
  2. J. Y. Mok, H. J. Kang, J. K. Cho, I. H. Jeon, H. S. Kim, J. M. Park, S. I. Jeong, J. S. Shin and S. I. Jang, "Antioxidative and antiinflammatory effects of extracts from different organs of Cirsium Japonicum var. ussuriense. Kor J. Herbology", Vol.26, pp. 39-47, (2011).
  3. J. R. Jang, S. Y. Hwang and S. Y. Lim, "Inhibitory effect of extracts of Platycodon grandiflorum on oxidation and nitric oxide production. Korean J. Food Preserv." Vol.18, pp. 65-71, (2011). https://doi.org/10.11002/kjfp.2011.18.1.065
  4. P. A. Cerutti, "Prooxidant states and tumor promotion", Science, Vol.227, pp. 375-381, (1985). https://doi.org/10.1126/science.2981433
  5. D. Juze and M. Van Montagu, "Oxidative stress in plants", Curr Opin Biotechnol. Vol.6, pp. 166-172, (1995).
  6. H. Y. Hsu, Y. P. Chen, S. J. Shen, C. S. Hsu, C. C. Chen and H. C. Chang, "Oriental material Medica. Oriental healing Art Institute", California USA. pp. 624, (1986).
  7. M. Zhu, K. F. Lin, R. Y. Yeung and R. C. Li, "Evaluation of the protective effects of Schizandra chinensis on phase I drug metabolism using a CCl4 intoxication model", J. Ethnopharmacol. vol.67, pp. 61-68, (1999). https://doi.org/10.1016/S0378-8741(98)00210-4
  8. J. D. Mellor, "Fundamentals of freeze drying, Academic press", London, England, pp. 244-250, (1978).
  9. S. I. Choi, Y. M. Lee and T. R. Heo, "Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extract". Korean J. Biotechnol. Bioeng., Vol.18. pp. 282-288, (2003).
  10. K. M. Kim, J. K. Hwang, K. M. H. S. and J. H. Song, "Detoxicating effects of oriental herbs extract mixtures on nicotine and dioxin". Korean J. Food Sci. Technol., Vol.35, pp. 980-987, (2003).
  11. H. K. Kim, Y. E. Kim, J. R. Do, Y. C. Lee and B. Y. Lee, "Antioxidative activity and physiological activity of some Korean medical plants". Korean J. Food Sci. Technol., Vol. 27, pp. 80-85, (1995).
  12. AOAC. Official Methods of Analysis of AOAC Int.16th ed. Association of Official Analytical Chemists. Washington, DC, USA (1995).
  13. Korean Soc. Food. Sci. Nutr. Food nutrition experiment handbook. Hyoil, pp. 124-126, (2000).
  14. AOAC. Official Methods Analysis 13th ed. Association of Official Analytical Chemists. Washington D.C. USA. pp. 125-132, (1990).
  15. Korean Food Standards Codex. 7. General experiment. 2-1 General composition experiment. pp. 18, (2012).
  16. H. Y. Cho and K. H. Kong, "Molecular cloning expression and characterization of a phi-type glutathione S-transferase from Oryza sativa". Pestic Biochem Phys, Vol.83, pp. 29-36 (2005). https://doi.org/10.1016/j.pestbp.2005.03.005
  17. H. Y. Cho," Studies on the biochemical charaterization and the application of plant-specific rice glutathione S-transferase". Ph.D. Thesis, Chung Ang Univ. Seoul, Korea.
  18. S. C. Oh, "Antioxidant Effects of Herbal Wine containing Acanthopanax sessiliflorus, Lycium chinense, Schizandra chinensis, Cuscutae semen, Rubus coreanum and Plantaginis semen". J. of Korean Oil Chemists Soc. Vol.33(4), pp. 693-697, (2016). https://doi.org/10.12925/jkocs.2016.33.4.693
  19. K. S. Lee and K. S. Park. "A study of glutathione S-transferase inhibitors obtained from Allium cepa var. cepa extract". Korean J Food & Nutr., Vol.26, pp. 725-730, (2013). https://doi.org/10.9799/ksfan.2013.26.4.725
  20. G. Batist, B. K. Sinha and A. G. "Katki, Differential formation of hydroxyl radicals by adriamycin in sensitive and resistant MCF-7 human breast tumor", Cells Biochem., Vol.26, pp. 3776-3780, (1987).
  21. W. E. Fahl, R. L. Schecter and M. A. Alaoui-Jamali, "Expression of a rat glutathione S-transferase complementary DNA in rat mammary carcinoma cells: Impact upon alkylation induced toxicity". Cancer Research Vol.53, pp. 4900 (1993).