Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of Oxidation Inhibition and Nitrogen Oxide Scavenging Activity from Curcuma longa L. Extracts

울금(Curcuma longa L.) 추출물의 산화억제 및 질소산화물 소거활성

  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • Received : 2019.01.23
  • Accepted : 2019.03.12
  • Published : 2019.03.31
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Abstract

The aim of the present investigation was to assess the oxidation inhibition by nitrogen oxide scavenging activity and physiological activities. Bioactive compound of proanthocyanidin $69.000{\pm}2.737mg$ catechin equivalents (CE)/g dry weight. Antioxidant effects (nitric oxide radical scavenging activity, nitrite scavenging activity, ${\beta}$-carotene bleaching assay and lipid peroxidation inhibition activity) of distilled water (DW), 70% ethanol and n-butanol extract of turmeric (Curcuma longa L.). Turmeric extracts yield were DW 17.11%, 70% ethanol 15.26% and n-butanol 4.12%, respectively. Oxidation inhibition activity of the samples exhibited a dose-dependent increase. However, in the current study, none of the samples evaluated showed activity as strong as the BHA and trolox. Total flavonoid content was the highest in the n-butanol extract, followed by 70% ethanol and DW extract. Further, nitrite scavenging activity was the highest for the n-butanol extract. As a result of this experiment, the turmeric can be utilized as a valuable and potential natural oxidation inhibition for the functional food industry.

울금(Curcuma longa L.)의 산화억제 및 질소산화물 소거활성 등 기능성 소재로서 활용 가능성을 검토한 결과, 프로안토시아니딘(proanthocyanidin) 함량은 $69.000{\pm}2.737mg$ catechin equivalents (CE)/g dry weight으로 확인되었으며, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올(n-butanol)의 3가지 용매를 사용한 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%) 순으로 관찰되었다. 총 플라보노이드(total flavonoid) 함량은 DW, 70% 에탄올 및 노르말 부탄올에서 각각 0.032, 0.512 및 2.221 mg quercetin equivalents (QE)/g의 함량으로 나타났고, 노르말 부탄올에서는 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). Nitric oxide (NO) 라디칼 소거 활성은 농도 별(0.2~0.8 mg/mL) DW에서 15.64~26.20%, 70% 에탄올 10.52~20.76%, 노르말 부탄올에서 13.39~69.92%로 확인되었다. Nitrite ($NO_2$) 소거활성은 DW 및 70% 에탄올과 비교하였을 때 노르말 부탄올에서 강한 $NO_2$ 소거활성을 보였다. ${\beta}$-carotene 탈색 저해활성은 DW에서 8.81~25.93%, 70% 에탄올 1.20~20.20%, 노르말 부탄올 12.08~43.93%로 나타났다. 지질과산화 저해활성은 DW, 70% 에탄올 및 노르말 부탄올에서 각각 5.60~27.54%, 37.78~50.79% 및 41.79~46.39%로 동정되었다. 이에, 천연 항산화제 등 기능성 소재로서의 활용 가능성이 있을 것으로 사료된다.

Keywords

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Fig. 1. Nitric oxide (NO) radical scavenging activity of various solvent extracts from turmeric (Curcuma longa L.).

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Fig. 2. Nitrite (NO2) scavenging activity of various solvent extracts from turmeric(Curcuma longa L.).

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Fig. 3. Antioxidant activity by β-carotene (BC) bleaching assay of various solvent extracts from turmeric (Curcuma longa L.).

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Fig. 4. Lipid peroxidation inhibition (LPI) activity of various solvent extracts from turmeric (Curcuma longa L.).

Table 1. Contents of proanthocyanidin and total flavonoid in the bioactivity evaluation assays from turmeric (Curcuma longa L.)

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Table 2. IC50 values of NO radical scavenging activity, NO2 scavenging activity, antioxidant activity by BC bleaching assay and LPI activity by turmeric (Curcuma longa L.)

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