The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Confirmation of Coleosporium zanthoxyli on Zanthoxylum ailanthoides in Korea

머귀나무에서 녹병균 Coleosporium zanthoxyli의 확인

  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Lee, Chong-Kyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Lee, Ho-Sang (Warm-Temperate and Subtropical Forest Research Center, National Institute of Forest Science) ;
  • Choi, Won-Il (Warm-Temperate and Subtropical Forest Research Center, National Institute of Forest Science)
  • 신현동 (고려대학교 환경생태공학부) ;
  • 최영준 (군산대학교 생물학과) ;
  • 이총규 (경남과학기술대학교 산림자원학과) ;
  • 이호상 (국립산림과학원 난대아열대산림연구소) ;
  • 최원일 (국립산림과학원 난대아열대산림연구소)
  • Received : 2019.01.30
  • Accepted : 2019.03.08
  • Published : 2019.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A previous record of Coleosporium phellodendri on Zanthoxylum ailanthoides in Korea by Korean researchers was regarded as C. zanthoxyli by foreign scientists without further studies. To clarify the identity of a Coleosporium species occurring on Z. ailanthoides in Korea, we examined the morphological characteristics of eight materials and analyzed the internal transcribed spacer (ITS) and 28S large subunit (LSU) rDNA regions of three representative specimens which were collected in Korea and deposited in the Mycological Herbarium at Korea University (Seoul, Korea). All specimens collected in Korea were morphologically and molecularly determined to be C. zanthoxyli. Therefore, this is the first confirmed report on the presence of C. zanthoxyli on Z. ailanthoides in Korea.

한국 학자들에 의해 머귀나무의 녹병균이 Coleosporium phellodendri로 보고되었으나, 추가적인 연구 없이 외국 학자들에 의해 이 녹병균이 C. zanthoxyli로 기록되었다. 한국에서 머귀나무 녹병균의 정체를 밝히기 위하여, 한국에서 채집되어 고려대학교 진균표본보관소에 보존된 8개 시료를 형태적으로 검경하고 대표적인 3개 시료의 internal transcribed spacer (ITS) 및 28S large subunit (LSU) rDNA 의 염기서열을 분석하였다. 그 결과, 한국의 머귀나무 녹병균은 형태적으로나 분자적으로 C. zanthoxyli로 동정하는 것이 옳다고 결정되었다. 따라서 이 연구를 통하여 머귀나무에 녹병균 C. zanthoxyli가 존재한다는 사실을 처음으로 보고한다.

Keywords

GNHHDL_2019_v47n1_83_f0001.png 이미지

Fig. 1. Coleosporium zanthoxyli occurring on Zanthoxylum ailanthoides. A, Symptoms appeared on the upper surface of the leaves. B, Uredinia formed on the lower surface of the leaves. C and D, Closeup view of erumpent uredinia. E and F, Urediniospores observed at different focal planes. G, Uredinium observed under scanning electron microscope. H and I, Urediniospores observed under scanning electron microscope.

GNHHDL_2019_v47n1_83_f0002.png 이미지

Fig. 2. Minimum evolution tree of Coleosporium species based on ITS rDNA sequences. Bootstrapping support values higher than 70% are given above the branches. The Korean specimens are shown in bold. The scale bar equals the number of nucleotide substitutions per site.

References

  1. Appelhans MS, Reichelt N, Groppo M, Paetzold C, Wen J. Phylogeny and biogeography of the pantropical genus Zanthoxylum and its closest relatives in the proto-Rutaceae group (Rutaceae). Mol Phylogenet Evol 2018;126:31-44. https://doi.org/10.1016/j.ympev.2018.04.013
  2. Lee YN, Lee KS, Shin YM. Wild plants of Jeju island. Seoul: Yeomiji: 2001.
  3. Farr DF, Rossman AY. Fungal databases, U.S. National Fungus Collections, ARS, USDA [Internet]. Beltsville: Systematic Mycology and Microbiology Laboratory; 2018 [cited 2018 Jul 25]. Available from: https://nt.ars-grin.gov/fungaldatabases/.
  4. Hiratsuka N, Sato S, Katsuya K, Kakishima M, Hiratsuka Y, Kaneko S, Ono Y, Sato T, Harada Y, Hiratsuka T, et al. The rust flora of Japan. Tsukuba: Tsukuba Shuppankai; 1992.
  5. Back CG, Nam GY, Kyukyuwin N, Lee SY, Kang IK, Lee SC, Jung HY, Ohga S. Characterization of Coleosporium phellodendri causing rust disease on Japanese prickly-ash tree. J Fac Agric Kyushu Univ 2012;57:379-82. https://doi.org/10.5109/25194
  6. Aime MC. Toward resolving family-level relationships in rust fungi (Uredinales). Mycoscience 2006;47:112-22. https://doi.org/10.1007/S10267-006-0281-0
  7. Beenken L, Zoller S, Berndt R. Rust fungi on Annonaceae II: the genus Dasyspora Berk. & M.A. Curtis. Mycologia 2012;104:659-81. https://doi.org/10.3852/11-068
  8. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  9. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for Bigger Datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  10. Zhu H, Huang YJ, Ji XP, Su T, Zhou ZK. Continuous existence of Zanthoxylum (Rutaceae) in Southwest China since the Miocene. Quatern Intl 2016;392:224-32. https://doi.org/10.1016/j.quaint.2015.05.020
  11. Shin HD, Jeon CH, Choi WI, Lee CK. First report of rust caused by Coleosporium zanthoxyli on Zanthoxylum planispinum in Korea. Plant Dis 2019;103:371.
  12. Hiratsuka N. Uredinales collected in Korea. V. Bot Mag Japan 1942;56:53-61. https://doi.org/10.15281/jplantres1887.56.53
  13. Lee SK, Kim KH, Lee CK, Kim DY, Hwang JH. Morphological features of Coleosporium zanthoxyli and its alternate host in Korea. Res Plant Dis 2004;10:279-84. https://doi.org/10.5423/RPD.2004.10.4.279