Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Removal of mycotoxin ochratoxin A by isolated bacteria

분리세균에 의한 진균독소 ochratoxin A의 제거

  • Choi, Ho-Yeong (Department of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • Received : 2018.11.19
  • Accepted : 2019.01.03
  • Published : 2019.03.31
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Abstract

Ochratoxin A (OTA), one of mycotoxins produced mainly by Aspergillus is a common contaminant of stored grains, posing health hazards to human and livestock. The aim of this study is to explore ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to remove OTA. AF13 and YR226 could remove 94.23 and 97.73% of OTA ($100{\mu}g/L$), respectively during 24 h incubation in NB medium. When cultures of two strains were separated into washed cells and cell-free supernatant, the supernatant of both strains removed more than 90% of $100{\mu}g/L$ OTA, and 98.88% of OTA could be also removed by the washed cells of YR226. OTA removal occurred in a few second by the supernatant of both strains, and treatments of autoclaving, proteinase K and chymotrypsin did not affect the OTA removal by the culture supernatants, which indicate that some thermostable and non-proteinaceous substances secreted by these bacteria may be involved in OTA removal in these two bacteria. These results suggest that AF13 and YR226 can be used to remove OTA from OTA-contaminated grains and feeds, and therefore decrease economic damage in agriculture and feed industry.

진균독소의 한 종류인 ochratoxin A (OTA)는 저장 곡물에 흔한 오염물질로 주로 Aspergillus에 의해 생성되어 인간과 가축의 건강을 위협한다. 이 연구의 목적은 분리 세균Bacillus subtilis AF13과 Streptomyces shenzhenensis YR226의 OTA 제거능을 조사하는 것이다. NB 배지에서 AF13과 YR226은 $100{\mu}g/L$ OTA를 24시간 동안 각각 94.23과 97.73% 제거하였다. 두 균주 배양액의 원심분리 후 분리된 세척 세포와 무세포 상등액의 OTA 제거능을 측정하였는데 두 균주의 상등액 모두 $100{\mu}g/L$ OTA를 수 초 내에 90% 이상 제거하였으며, YR226의 세척 세포도 OTA를 24시간에 98.88% 제거하였다. 배양 상등액의 고압멸균, proteinase K와 chymotrypsin 처리 시 그 OTA 제거능은 영향을 받지 않았으며 이는 두 균주들에 의해 분비된 열-안정성을 가진 비-단백질성 물질이 OTA 제거에 관련된 것을 추정할 수 있다. 이 결과는 AF13과 YR226 균주가 OTA로 오염된 곡물과 사료에서 OTA를 제거하는데 사용될 수 있으며 따라서 농업과 사료 산업에서 경제적 피해를 감소시킬 수 있을 것이다.

Keywords

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Fig. 1. OTA (100 μg/L) removal by culture supernatant (■) and washed cells (□) of AF13 and YR226 in phosphate buffer (37℃, 150 rpm, 24 h). ***, significant at P < 0.001.

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Fig. 2. Time course of OTA (100 μg/L) removal by culture supernatant of AF13 (○) and YR226 (◇) at 37℃.

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Fig. 3. Time course of OTA (100 μg/L) removal by washed cells of YR226 (◇) (37℃, 150 rpm, 72 h). **, significant at P < 0.01; ***, significant at P < 0.001.

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Fig. 4. Effect of temperature on OTA (100 μg/L) removal in culture of AF13 (○) and YR226 (◇) (150 rpm, 24 h).

Table 1. Effect of treatment of heat, proteinase K (1 mg/ml) and chymotrypsin (1 mg/ml) on OTA (100 µg/L) removal by culture supernatant of strains AF13 and YR226

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