The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of sec-O-glucosylhamaudol on mechanical allodynia in a rat model of postoperative pain

  • Koh, Gi-Ho (Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Song, Hyun (Department of Anesthesiology and Pain Medicine, Chosun University Hospital) ;
  • Kim, Sang Hun (Department of Anesthesiology and Pain Medicine, Chosun University Hospital) ;
  • Yoon, Myung Ha (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University) ;
  • Lim, Kyung Joon (Department of Anesthesiology and Pain Medicine, Chosun University Hospital) ;
  • Oh, Seon-Hee (School of Medicine, Chosun University) ;
  • Jung, Ki Tae (Department of Anesthesiology and Pain Medicine, Chosun University Hospital)
  • Received : 2019.01.14
  • Accepted : 2019.03.05
  • Published : 2019.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Background: This study was performed in order to examine the effect of intrathecal sec-O-glucosylhamaudol (SOG), an extract from the root of the Peucedanum japonicum Thunb., on incisional pain in a rat model. Methods: The intrathecal catheter was inserted in male Sprague-Dawley rats (n = 55). The postoperative pain model was made and paw withdrawal thresholds (PWTs) were evaluated. Rats were randomly treated with a vehicle (70% dimethyl sulfoxide) and SOG ($10{\mu}g$, $30{\mu}g$, $100{\mu}g$, and $300{\mu}g$) intrathecally, and PWT was observed for four hours. Dose-responsiveness and ED50 values were calculated. Naloxone was administered 10 min prior to treatment of SOG $300{\mu}g$ in order to assess the involvement of SOG with an opioid receptor. The protein levels of the ${\delta}$-opioid receptor, ${\kappa}$-opioid receptor, and ${\mu}$-opioid receptor (MOR) were analyzed by Western blotting of the spinal cord. Results: Intrathecal SOG significantly increased PWT in a dose-dependent manner. Maximum effects were achieved at a dose of $300{\mu}g$ at 60 min after SOG administration, and the maximal possible effect was 85.35% at that time. The medial effective dose of intrathecal SOG was $191.3{\mu}g$ (95% confidence interval, 102.3-357.8). The antinociceptive effects of SOG ($300{\mu}g$) were significantly reverted until 60 min by naloxone. The protein levels of MOR were decreased by administration of SOG. Conclusions: Intrathecal SOG showed a significant antinociceptive effect on the postoperative pain model and reverted by naloxone. The expression of MOR were changed by SOG. The effects of SOG seem to involve the MOR.

Keywords

References

  1. Pyati S, Gan TJ. Perioperative pain management. CNS Drugs 2007; 21: 185-211. https://doi.org/10.2165/00023210-200721030-00002
  2. Kang S, Brennan TJ. Mechanisms of postoperative pain. Anesth Pain Med 2016; 11: 236-48. https://doi.org/10.17085/apm.2016.11.3.236
  3. Shin DJ, Yoon MH, Lee HG, Kim WM, Park BY, Kim YO, et al. The effect of treatment with intrathecal ginsenosides in a rat model of postoperative pain. Korean J Pain 2007; 20:100-5. https://doi.org/10.3344/kjp.2007.20.2.100
  4. Kim IJ, Park CH, Lee SH, Yoon MH. The role of spinal adrenergic receptors on the antinociception of ginsenosides in a rat postoperative pain model. Korean J Anesthesiol 2013; 65: 55-60. https://doi.org/10.4097/kjae.2013.65.1.55
  5. Yeager MP, Glass DD, Neff RK, Brinck-Johnsen T. Epidural anesthesia and analgesia in high-risk surgical patients. Anesthesiology 1987; 66: 729-36. https://doi.org/10.1097/00000542-198706000-00004
  6. Pogatzki-Zahn EM, Segelcke D, Schug SA. Postoperative pain-from mechanisms to treatment. Pain Rep 2017; 2:e588. https://doi.org/10.1097/PR9.0000000000000588
  7. McCurdy CR, Scully SS. Analgesic substances derived from natural products (natureceuticals). Life Sci 2005; 78: 476-84. https://doi.org/10.1016/j.lfs.2005.09.006
  8. Ikeshiro Y, Mase I, Tomia Y. Dihydropyranocoumarins from roots of Peucedanum japonicum. Phytochemistry 1992; 31: 4303-6. https://doi.org/10.1016/0031-9422(92)80463-O
  9. Chen IS, Chang CT, Sheen WS, Teng CM, Tsai IL, Duh CY, et al. Coumarins and antiplatelet aggregation constituents from Formosan Peucedanum japonicum. Phytochemistry 1996; 41: 525-30. https://doi.org/10.1016/0031-9422(95)00625-7
  10. Hisamoto M, Kikuzaki H, Ohigashi H, Nakatani N. Antioxidant compounds from the leaves of Peucedanum japonicum thunb. J Agric Food Chem 2003; 51: 5255-61. https://doi.org/10.1021/jf0262458
  11. Zimecki M, Artym J, Cisowski W, Mazol I, WIodarczyk M, Glensk M. Immunomodulatory and anti-inflammatory activity of selected osthole derivatives. Z Naturforsch C 2009; 64: 361-8.
  12. Zheng MS, Jin WY, Son KH, Chang HW, Kim HP, Bae KH, et al. The constituents isolated from Peucedanum japonicum Thunb. and their Cyclooxygenase (COX) inhibitory activity. Korean J Med Crop Sci 2005; 13: 75-9.
  13. Okuyama E, Hasegawa T, Matsushita T, Fujimoto H, Ishibashi M, Yamazaki M. Analgesic components of saposhnikovia root (Saposhnikovia divaricata). Chem Pharm Bull (Tokyo) 2001; 49: 154-60. https://doi.org/10.1248/cpb.49.154
  14. Kim SH, Jong HS, Yoon MH, Oh SH, Jung KT. Antinociceptive effect of intrathecal sec-O-glucosylhamaudol on the formalininduced pain in rats. Korean J Pain 2017; 30: 98-103. https://doi.org/10.3344/kjp.2017.30.2.98
  15. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983; 16: 109-10. https://doi.org/10.1016/0304-3959(83)90201-4
  16. Yaksh TL, Rudy TA. Chronic catheterization of the spinal subarachnoid space. Physiol Behav 1976; 17: 1031-6. https://doi.org/10.1016/0031-9384(76)90029-9
  17. Brennan TJ, Vandermeulen EP, Gebhart GF. Characterization of a rat model of incisional pain. Pain 1996; 64: 493-501. https://doi.org/10.1016/0304-3959(95)01441-1
  18. Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods 1994; 53: 55-63. https://doi.org/10.1016/0165-0270(94)90144-9
  19. Tallarida RJ, Murray RB. Manual of pharmacologic calculations with computer programs. 2nd ed. New York, Springer-Verlag. 1987, pp 1-95.
  20. Garimella V, Cellini C. Postoperative pain control. Clin Colon Rectal Surg 2013; 26: 191-6. https://doi.org/10.1055/s-0033-1351138
  21. Woolf CJ, Chong MS. Preemptive analgesia: treating postoperative pain by preventing the establishment of central sensitization. Anesth Analg 1993; 77: 362-79. https://doi.org/10.1213/00000539-199377020-00026
  22. Law PY, Reggio PH, Loh HH. Opioid receptors: toward separation of analgesic from undesirable effects. Trends Biochem Sci 2013; 38: 275-82. https://doi.org/10.1016/j.tibs.2013.03.003
  23. Xiao X, Wang X, Gui X, Chen L, Huang B. Natural flavonoids as p romising a nalgesic c andidates: a s ystematic review. Chem Biodivers 2016; 13: 1427-40. https://doi.org/10.1002/cbdv.201600060
  24. Khadem S, Marles RJ. Chromone and flavonoid alkaloids: occurrence and bioactivity. Molecules 2011; 17: 191-206. https://doi.org/10.3390/molecules17010191
  25. Colak T, Akca T, Dirlik M, Kanik A, Dag A, Aydin S. Micronized flavonoids in pain control after hemorrhoidectomy: a prospective randomized controlled study. Surg Today 2003; 33: 828-32. https://doi.org/10.1007/s00595-003-2604-5
  26. Silva CF, Pinto DC, Silva AM. Chromones: a promising ring system for new anti-inflammatory drugs. ChemMedChem 2016; 11: 2252-60. https://doi.org/10.1002/cmdc.201600359
  27. Sarkhail P. Traditional uses, phytochemistry and pharmacological properties of the genus Peucedanum: a review. J Ethnopharmacol 2014; 156: 235-70. https://doi.org/10.1016/j.jep.2014.08.034
  28. Sasaki H, Taguchi H, Endo T, Yosioka I. The constituents of Ledebouriella seseloides WOLFF. I. Structures of three new chromones. Chem Pharm Bull (Tokyo) 1982; 30: 3555-62. https://doi.org/10.1248/cpb.30.3555
  29. Gautam R, Jachak SM, Kumar V, Mohan CG. Synthesis, biological evaluation and molecular docking studies of stellatin derivatives as cyclooxygenase (COX-1, COX-2) inhibitors and anti-inflammatory agents. Bioorg Med Chem Lett 2011; 21: 1612-6. https://doi.org/10.1016/j.bmcl.2011.01.116
  30. Chun JM, Kim HS, Lee AY, Kim SH, Kim HK. Antiinflammatory and antiosteoarthritis effects of saposhnikovia divaricata ethanol extract: in vitro and in vivo studies. Evid Based Complement Alternat Med 2016; 2016: 1984238.
  31. de Oliveira Junior JO, de Freitas MF, Bullara de Andrade C, Chacur M, Ashmawi HA. Local analgesic effect of tramadol is mediated by opioid receptors in late postoperative pain after plantar incision in rats. J Pain Res 2016; 9: 797-802. https://doi.org/10.2147/JPR.S117674
  32. Berger V, Alloui A, Kemeny JL, Dubray C, Eschalier A, Lavarenne J. Evidence for a role for bulbospinal pathways in the spinal antinociceptive effect of systemically administered vapreotide in normal rats. Fundam Clin Pharmacol 1998; 12: 200-4. https://doi.org/10.1111/j.1472-8206.1998.tb00942.x
  33. Katavic PL, Lamb K, Navarro H, Prisinzano TE. Flavonoids as opioid receptor ligands: identification and preliminary structure-activity relationships. J Nat Prod 2007; 70: 1278-82. https://doi.org/10.1021/np070194x
  34. Higgs J, Wasowski C, Loscalzo LM, Marder M. In vitro binding affinities of a series of flavonoids for $\mu$-opioid receptors. Antinociceptive effect of the synthetic flavonoid 3,3-dibromoflavanone in mice. Neuropharmacology 2013; 72: 9-19. https://doi.org/10.1016/j.neuropharm.2013.04.020
  35. Colucci M, Maione F, Bonito MC, Piscopo A, Di Giannuario A, Pieretti S. New insights of dimethyl sulphoxide effects (DMSO) on experimental in vivo models of nociception and inflammation. Pharmacol Res 2008; 57: 419-25. https://doi.org/10.1016/j.phrs.2008.04.004

Cited by

  1. Antinociceptive effects of intrathecal cimifugin treatment: a preliminary rat study based on formalin test vol.15, pp.4, 2019, https://doi.org/10.17085/apm.20032
  2. Prostaglandin D2 contributes to cisplatin-induced neuropathic pain in rats via DP2 receptor in the spinal cord vol.34, pp.1, 2019, https://doi.org/10.3344/kjp.2021.34.1.27