The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
권호 표지
DOI QR코드

DOI QR Code

Analgesic effects of eucalyptus essential oil in mice

  • Lee, Ganggeun (Department of Anesthesiology and Pain Medicine, Eulji University Hospital) ;
  • Park, Junbum (Department of Anesthesiology and Pain Medicine, Eulji University Hospital) ;
  • Kim, Min Sun (Department of Physiology and Biophysics, Eulji University School of Medicine) ;
  • Seol, Geun Hee (Department of Basic Nursing Science, Korea University School of Nursing) ;
  • Min, Sun Seek (Department of Physiology and Biophysics, Eulji University School of Medicine)
  • Received : 2018.10.15
  • Accepted : 2019.01.21
  • Published : 2019.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Background: The use of aroma oils dates back to at least 3000 B.C., where it was applied to mummify corpses and treat the wounds of soldiers. Since the 1920s, the term "aromatherapy" has been used for fragrance therapy with essential oils. The purpose of this study was to determine whether the essential oil of Eucalyptus (EOE) affects pain pathways in various pain conditions and motor coordination. Methods: Mice were subjected to inhalation or intraperitoneal injection of EOE, and its analgesic effects were assessed by conducting formalin, thermal plantar, and acetic acid tests; the effects of EOE on motor coordination were evaluated using a rotarod test. To determine the analgesic mechanism, 5'-guanidinonaltrindole (${\kappa}$-opioid antagonist, 0.3 mg/kg), naltrindole (${\delta}$-opioid antagonist, 5 mg/kg), glibenclamide (${\delta}$-opioid antagonist, 2 mg/kg), and naloxone (${\mu}$-opioid antagonist, 4, 8, 12 mg/kg) were injected intraperitoneally. Results: EOE showed an analgesic effect against visceral pain caused by acetic acid (EOE, 45 mg/kg); however, no analgesic effect was observed against thermal nociceptive pain. Moreover, it was demonstrated that EOE did not have an effect on motor coordination. In addition, an anti-inflammatory effect was observed during the formalin test. Conclusions: EOE, which is associated with the ${\mu}$-opioid pain pathway, showed potential effects against somatic, inflammatory, and visceral pain and could be a potential therapeutic agent for pain.

Keywords

References

  1. Marchand S, Goffaux P. Pain processing in the cingulate cortex, behavioral studies in humans. In: Encyclopedia of pain. Edited by Schmidt RF, Willis WD. Berlin, Heidelberg, Springer. 2007, pp 1732-4.
  2. Cockcroft A, Cosgrove JB, Wood RJ. Comparative repellency of commercial formulations of deet, permethrin and citronellal against the mosquito Aedes aegypti, using a collagen membrane technique compared with human arm tests. Med Vet Entomol 1998; 12: 289-94. https://doi.org/10.1046/j.1365-2915.1998.00121.x
  3. Juergens UR, Stober M, Schmidt-Schilling L, Kleuver T, Vetter H. Antiinflammatory effects of euclyptol (1.8-cineole) in bronchial asthma: inhibition of arachidonic acid metabolism in human blood monocytes ex vivo. Eur J Med Res 1998; 3: 407-12.
  4. Trigg JK. Evaluation of a eucalyptus-based repellent against Anopheles spp. in Tanzania. J Am Mosq Control Assoc 1996; 12: 243-6.
  5. Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 1987; 30: 103-14. https://doi.org/10.1016/0304-3959(87)90088-1
  6. Quintans-Junior L, Moreira JC, Pasquali MA, Rabie SM, Pires AS, Schroder R, et al. Antinociceptive activity and redox profile of the monoterpenes (+)-camphene, p-cymene, and geranyl acetate in experimental models. ISRN Toxicol 2013; 2013: 459530. https://doi.org/10.1155/2013/459530
  7. Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 1988; 32: 77-88. https://doi.org/10.1016/0304-3959(88)90026-7
  8. Silva J, Abebe W, Sousa SM, Duarte VG, Machado MI, Matos FJ. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol 2003; 89: 277-83. https://doi.org/10.1016/j.jep.2003.09.007
  9. Daniel AN, Sartoretto SM, Schmidt G, Capparoz-Assef SM, Bersani-Amado CA, Cuman RK. Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models. Rev Bras Farmacogn 2009; 19: 212-7. https://doi.org/10.1590/S0102-695X2009000200006
  10. Abdollahi M, Karimpour H, Monsef-Esfehani HR. Antinociceptive effects of Teucrium polium L total extract and essential oil in mouse writhing test. Pharmacol Res 2003; 48: 31-5. https://doi.org/10.1016/S1043-6618(03)00059-8
  11. Bannerman DM, Rawlins JN, McHugh SB, Deacon RM, Yee BK, Bast T, et al. Regional dissociations within the hippocampus: memory and anxiety. Neurosci Biobehav Rev 2004; 28: 273-83. https://doi.org/10.1016/j.neubiorev.2004.03.004
  12. Hobin JA, Ji J, Maren S. Ventral hippocampal muscimol disrupts context-specific fear memory retrieval after extinction in rats. Hippocampus 2006; 16: 174-82. https://doi.org/10.1002/hipo.20144
  13. Trivedi MA, Coover GD. Lesions of the ventral hippocampus, but not the dorsal hippocampus, impair conditioned fear expression and inhibitory avoidance on the elevated T-maze. Neurobiol Learn Mem 2004; 81: 172-84. https://doi.org/10.1016/j.nlm.2004.02.005
  14. Ness TJ, Gebhart GF. Visceral pain: a review of experimental studies. Pain 1990; 41: 167-234. https://doi.org/10.1016/0304-3959(90)90021-5
  15. Cervero F. Sensory innervation of the viscera: peripheral basis of visceral pain. Physiol Rev 1994; 74: 95-138. https://doi.org/10.1152/physrev.1994.74.1.95
  16. Ferreira SH. A classification of peripheral analgesics based upon their mode of action. In: Migraine: a spectrum of ideas. Edited by Sandler M, Collins GM. Oxford, Oxford University Press. 1990, pp 59-72.
  17. Viana GS, Vale TG, Pinho RS, Matos FJ. Antinociceptive effect of the essential oil from Cymbopogon citratus in mice. J Ethnopharmacol 2000; 70: 323-7. https://doi.org/10.1016/S0378-8741(99)00168-3
  18. Liapi C, Anifandis G, Chinou I, Kourounakis AP, Theodosopoulos S, Galanopoulou P. Antinociceptive properties of 1,8-Cineole and beta-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in rodents. Planta Med 2007; 73: 1247-54. https://doi.org/10.1055/s-2007-990224

Cited by

  1. Development of New Formula Microcapsules from Nutmeg Essential Oil Using Sucrose Esters and Magnesium Aluminometasilicate vol.12, pp.7, 2019, https://doi.org/10.3390/pharmaceutics12070628
  2. Efficacy of Essential Oils in Pain: A Systematic Review and Meta-Analysis of Preclinical Evidence vol.12, 2019, https://doi.org/10.3389/fphar.2021.640128
  3. A systematic and comprehensive review on current understanding of the pharmacological actions, molecular mechanisms, and clinical implications of the genus Eucalyptus vol.1, pp.4, 2019, https://doi.org/10.1016/j.phyplu.2021.100089