Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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In vitro Multiplication of Hosta Tratt. Species Native to Korea by Shoot-tip Culture

경정배양에 의한 한국 자생 비비추속 식물의 기내증식

  • Choi, Han (Useful Plant Resources Center, Korea National Arboretum) ;
  • Yang, Jong Cheol (Useful Plant Resources Center, Korea National Arboretum) ;
  • Ryu, Sun Hee (Useful Plant Resources Center, Korea National Arboretum) ;
  • Yoon, Sae Mi (Useful Plant Resources Center, Korea National Arboretum) ;
  • Kim, Sang Yong (Useful Plant Resources Center, Korea National Arboretum) ;
  • Lee, Seung Youn (Useful Plant Resources Center, Korea National Arboretum)
  • 최한 (국립수목원 유용식물증식센터) ;
  • 양종철 (국립수목원 유용식물증식센터) ;
  • 류선희 (국립수목원 유용식물증식센터) ;
  • 윤새미 (국립수목원 유용식물증식센터) ;
  • 김상용 (국립수목원 유용식물증식센터) ;
  • 이승연 (국립수목원 유용식물증식센터)
  • Received : 2018.09.15
  • Accepted : 2018.12.28
  • Published : 2019.02.28
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Abstract

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

본 연구는 우리나라에 자생하고 있는 비비추속 식물 6종(일월비비추, 주걱비비추, 다도해비비추, 좀비비추, 한라비비추, 흑산도비비추)의 정단을 이용하여 대량증식과 품종개발 등을 위한 기내증식체계를 확립하고자 하였다. 정단은 0.5, 1.0, 2.0, 4.0 mg/L BA와 0.1, 0.5, 1.0, 2.0 mg/L TDZ를 0.1 mg/L NAA와 각각 혼용한 조건과 PGRs을 무첨가한 조건(control)의 MS배지에 배양하였다. 배양 8주 후에 embryogenic callus, somatic embryo, crown bud, 그리고 신초와 뿌리의 분화 및 생육, 생체중 등에 대하여 조사하였다. 6종의 비비추 식물 모두에서 control에 비하여 PGRs 처리구의 embryogenic callus와 somatic embryo 형성율, 다신초 분화율이 높았다. 분화된 신초의 개수는 일월비비추는 2.0 mg/L TDZ에서 5.4개, 주걱비비추와 다도해비비추는 1.0 mg/L TDZ에서 각각 3.3개, 5.8개, 좀비비추는 0.5mg/L BA에서 11.1개, 흑산도비비추는 0.5 mg/L TDZ에서 9.8개, 한라비비추는 1.0 mg/L BA, 0.1 mg/L TDZ에서 8.1개로 가장 많았다. Somatic embryo 형성에서는 다도해비비추와 흑산도비비추가 처리한 PGRs에 대해 효과적이었고, 일월비비추, 주걱비비추, 좀비비추, 한라비비추에서는 상대적으로 효과가 적었다. 4종의 자생 비비추(일월비비추, 주걱비비추, 다도해비비추, 흑산도비비추)에서 조사된 crown bud도 control에 비하여 PGRs 처리구에서 더많이 형성되었다. 주걱비비추는 cytokinin의 종류 및 농도와 상관없이 callus와 신초 분화에 큰 효과가 나타나지 않았지만, crown bud의 형성에는 TDZ에서 약간 증가하였다.

Keywords

JOSMBA_2019_v32n1_53_f0001.png 이미지

Fig. 1. Effects of different concentrations and combinations of PGRs on number of somatic embryos (A), number of crown buds (B), number of shoots (C), and length of shoot (D) of in vitro seedlings derived from shoot tip of six Hosta species after 8 weeks of culture. ND, No data.

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Fig. 2. Formation of embryogenic callus and somatic embryo (indicated with red arrows) and crown bud (indicated with black arrows) on medium with 0.1 ㎎/L NAA and 1.0 ㎎/L TDZ combination in four Hosta species. Scale bars = 1 ㎜.

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Fig. 3. Effects of different concentrations and combinations of PGRs on growth of seedlings derived from shoot tip of six Hosta species after 8 weeks of culture. All media contain NAA 0.1 ㎎/L. Scale bars = 1 ㎝.

Table 1. Abbreviations and source of Hosta species used in this study

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Table 2. Effects of different concentrations and combinations of PGRs on in vitro multiplication from shoot tip of six Hosta species after 8 weeks of culture

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Table 3. Effects of different concentrations and combinations of PGRs on in vitro rooting of six Hosta species after 8 weeks of culture

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