The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative and Hepatocyte Protective Effects of Guava (Psidium guajava L.) Leaves Cultivated in Korea

국내산 구아바(Psidium guajava L.) 잎 추출물의 항산화 활성 및 간세포 보호효과

  • Cheon, Wonyoung (Dept. of Food Science and Biotechnology, Kyungsung University) ;
  • Seo, Dongyeon (Safety Analysis Div., Experiment Research Institute, National Agricultural Products Quality Management Service) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 천원영 (경성대학교 식품생명공학과 대학원) ;
  • 서동연 (국립농산물품질관리원 시험연구소 안전성분석과) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2019.01.03
  • Accepted : 2019.01.24
  • Published : 2019.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate the antioxidant and hepatocyte protective effects of guava (Psidium guajava L.) leaves cultivated in Korea. The contents of the total polyphenol of the extract was 271.57 mg gallic acid equivalent (GAE)/g residue. Antioxidant activities of leaf extract were evaluated by examining the free radical scavenging ability. 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ${\alpha}-{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were 1133.23 mg trolox equivalent antioxidant capacity (TEAC)/g residue and 721.68 mg TEAC/g residue, respectively. The hepatocyte protective effect of guava leaf extract was examined in HepG2 cells. Against tert-butyl hydroperoxide (TBHP), the viability of HepG2 cells were increased by the treatment of leaf extract. In addition, guava leaf extract led to the inhibition of reactive oxygen species (ROS) generated in HepG2 cells. The leaf extract increased the activity of glutathione (GSH), glutathione reductase (GR), and glutathione peroxidase (GPx) against oxidative stress. These results suggested that guava leaves might be regarded as a potential source natural antioxidant and a hepatoprotective material.

Keywords

HGSPB1_2019_v32n1_33_f0001.png 이미지

Fig. 2. Effect of methanol extract from Psidium guajava L. leaf on the intracellular reactive oxygen species (ROS) formation induced by tert-butyl hydroperoxide (TBHP).

HGSPB1_2019_v32n1_33_f0002.png 이미지

Fig. 3. Effect of methanol extract from Psidium guajava L. leaf on tert-butyl hydroperoxide (TBHP)-induced cellular redox state impairment.

HGSPB1_2019_v32n1_33_f0003.png 이미지

Fig. 4. Antioxidant enzyme activity of glutathione reductase (A) and glutathione peroxidase (B) were evaluated in HepG2 cells treated with 1 mM tert-butyl hydroperoxide (TBHP).

HGSPB1_2019_v32n1_33_f0004.png 이미지

Fig. 1. (A) Cytotoxicity and (B) cell protective effect of methanol extract from Psidium guajava L. leaf methanol extracts in HepG2 cells.

Table 1. Total polyphenols and flavonoid contents of the methanolic extract of Psidium guajava L. leaf.

HGSPB1_2019_v32n1_33_t0001.png 이미지

Table 2. ABTS and DPPH free radical scavenging activity of the methanolic extract of Psidium guajava L. leaf.

HGSPB1_2019_v32n1_33_t0002.png 이미지

References

  1. Aruoma OI. 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 75:199-212 https://doi.org/10.1007/s11746-998-0032-9
  2. Baker MA, Cerniglia GJ, Zaman A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190: 360-365 https://doi.org/10.1016/0003-2697(90)90208-Q
  3. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  4. Cederbaum AI, Lu Y, Wu D. 2009. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol 83:519-548 https://doi.org/10.1007/s00204-009-0432-0
  5. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12:239-243 https://doi.org/10.1016/S0021-9258(18)88697-5
  6. Halliwell B, Gutteridge JMC. 1999. Free Radicals in Biology and Medicine. 3rd ed. pp.105-350. Oxford University Press
  7. Hsieh CL, Huang CN, Lin YC, Peng RY. 2007. Molecular action mechanism against apoptosis by aqueous extract from guava budding leaves elucidated with human umbilical vein endothelial cell (HUVEC) model. J Agric Food Chem 55: 8523-8533 https://doi.org/10.1021/jf071858b
  8. Jeong EJ, Kim KP, Bang BH. 2012. Quality characteristics of cookies added with guava (Psidium guajava L.) leaf powder. Korean J Food Nutr 25:317-323 https://doi.org/10.9799/ksfan.2012.25.2.317
  9. Jiao HL, Ye P, Zhao BL. 2003. Protective effects of green tea polyphenols on human HepG2 cells against oxidative damage of fenofibrate. Free Radical Biol Med 35:1121-1128 https://doi.org/10.1016/S0891-5849(03)00506-9
  10. Jin YJ, Kang SH, Choi SY, Park SY, Park JG, Moon SW, Park DB, Kim SJ. 2006. Effect of fermented guava (Psidium guajava L.) leaf extract on hyperglycemia in low dose streptozotocin-induced diabetic mice. Korean J Food Sci Technol 38:679-683
  11. Jo YH, Ok DL, Lee SC. 2009. Antimicrobial characteristics of different parts of guava against food-borne bacteria. J Korean Soc Food Sci Nutr 38:1773-1778 https://doi.org/10.3746/jkfn.2009.38.12.1773
  12. Lee KM, Kwon TY, Kang U, Seo EK, Yun JH, Nho CW, Kim YS. 2017. Tussilagonone-induced Nrf2 pathway activation protects HepG2 cells from oxidative injury. Food Chem Toxicol 108:120-127 https://doi.org/10.1016/j.fct.2017.07.035
  13. Lozoya X, Reyes-Morales H, Chavez-Soto MA, Martinez-Garcia MDC, Soto-Gonzalez Y, Doubova SV. 2002. Intestinal antispasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease. J Ethnopharmacol 83:19-24 https://doi.org/10.1016/S0378-8741(02)00185-X
  14. Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem 100:1409-1418 https://doi.org/10.1016/j.foodchem.2005.11.032
  15. Ministry of Food and Drug Safety. 2016. Health Functional Food Code. p.2,11,96. Ministry of Food and Drug Safety
  16. Mosbah H, Louati H, Boujbiha MA, Chahdoura H, Snoussi M, Flamini G, Ascrizzi R, Bouslema A, Achour L, Selmi B. 2018. Phytochemical characterization, antioxidant, antimicrobial and pharmacological activities of Feijoa sellowiana leaves growing in Tunisia. Ind Crops Prod 112:521-531 https://doi.org/10.1016/j.indcrop.2017.12.051
  17. Moskaug JO, Carlsen H, Myhrstad MCW, Blomhoff R. 2005. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 81:277-283 https://doi.org/10.1093/ajcn/81.1.277S
  18. Nantitanon W, Yotsawimonwat S, Okonogi S. 2010. Factors influencing antioxidant activities and total phenolic content of guava leaf extract. LWT-Food Sci Technol 43:1095-1103 https://doi.org/10.1016/j.lwt.2010.02.015
  19. Nishida K, Ohta Y, Ishiguro I. 1997. Modulating role of endogenous reduced glutathione in tert-butyl hydroperoxide- induced cell injury in isolated rat hepatocytes. Free Radical Biol Med 23:453-462 https://doi.org/10.1016/S0891-5849(97)00116-0
  20. Pandey KB, Rizvi SI. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2:270-278 https://doi.org/10.4161/oxim.2.5.9498
  21. Park BJ, Onjo M. 2008. Antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Korean J Plant Resour 21:408-412
  22. Paul LW. 1999. Biochemical diagnosis of liver disease. Indian J. Clin Biochem 14:59-90 https://doi.org/10.1007/BF02869152
  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  24. Rees MD, Kennett EC, Whitelock JM, Davies MJ. 2008. Oxidative damage to extracellular matrix and its role in human pathologies. Free Radical Biol Med 44:1973-2001 https://doi.org/10.1016/j.freeradbiomed.2008.03.016
  25. Sies H. 1999. Glutathione and its role in cellular functions. Free Radical Biol Med 27:916-921 https://doi.org/10.1016/S0891-5849(99)00177-X
  26. Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. 2007. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 103:381-388 https://doi.org/10.1016/j.foodchem.2006.07.034
  27. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  28. Yeon SH, Ham H, Sung J, Kim Y, Namkoong S. Jeong HS, Lee J. 2013. Antioxidant activities of hot water extract from Cornus walteri Wanger against oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells. J Korean Soc Food Sci Nutr 42:1525-1532 https://doi.org/10.3746/jkfn.2013.42.10.1525
  29. Yoon TJ, Jo SY. 2010. Effect of acanthopanax senticosus extracts on alcohol degradation and anti-inflammatory activity in mice. Korean J Food Nutr 4:542-548
  30. Zhang XH, Choi SK, Seo JS. 2009. Effect of dietary grape pomace on lipid oxidation and related enzyme activities in rats fed high fat diet. Korean J Nutr 42:415-422 https://doi.org/10.4163/kjn.2009.42.5.415
  31. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2

Cited by

  1. 산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능 vol.35, pp.6, 2019, https://doi.org/10.7318/kjfc/2020.35.6.597