Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Anti-inflammatory Effects of Metformin on Neuro-inflammation and NLRP3 Inflammasome Activation in BV-2 Microglial Cells

  • Ha, Ji-Sun (Department of Biomedical Laboratory Science, Konyang University) ;
  • Yeom, Yun-Seon (Department of Biomedical Laboratory Science, Konyang University) ;
  • Jang, Ju-Hun (Department of Biomedical Laboratory Science, Konyang University) ;
  • Kim, Yong-Hee (Department of Biomedical Laboratory Science, Konyang University) ;
  • Im, Ji In (Department of Biomedical Laboratory Science, Konyang University) ;
  • Kim, In Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Yang, Seung-Ju (Department of Biomedical Laboratory Science, Konyang University)
  • Received : 2019.02.28
  • Accepted : 2019.03.22
  • Published : 2019.03.31
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Abstract

Metformin is a drug used for the treatment of diabetes and is associated with anti-inflammatory reaction, but the underlying mechanism is unclear. In this study, we investigated the effect of metformin on the inflammatory response in BV-2 microglial cells induced by lipopolysaccharide (LPS) and S100 calcium-binding protein A8 (S100A8). The results revealed that metformin significantly attenuated several inflammatory responses in BV-2 microglial cells, including the secretion of pro-inflammatory cytokines, such as tumor necrosis factor-${\alpha}$ and interleukin (IL)-6, involved in the activation of Beclin-1, a crucial regulator of autophagy. In addition, metformin inhibited the LPS-induced phosphorylation of ERK. Metformin also suppressed the activation of NOD-like receptor pyrin domain containing 3 inflammasomes composed of NLRP3, caspase-1, and apoptosis-associated speck like protein containing a caspase recruitment domain, which are involved in the innate immune response. Notably, metformin decreased the secretion of S100A8-induced IL-6 production. These findings suggest that metformin alleviates the neuroinflammatory response via autophagy activation.

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References

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