Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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The Ameliorative Effect of Angelica acutiloba Ameliorates Through the NO / iNOS Pathway in Rats with Systemic Allergy

  • Cho, Min-Seok (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Park, Hae-Woong (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Lee, Gil-Hyun (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Yoon, Hae-Gyung (Division of Basic Sciences, Dong-eui University) ;
  • Oh, Kyung-Mo (Department of Sports Leisure, Busan Kyungsang College) ;
  • Choi, Seok-Cheol (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Hyun, Kyung-Yae (Department of Clinical Laboratory Science, Dong-Eui University)
  • Received : 2019.02.28
  • Accepted : 2019.03.18
  • Published : 2019.03.31
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Abstract

This study was undertaken to investigate the therapeutic effect of Angelica acutiloba on allergic dermatitis, which Angelica acutiloba is traditionally known to have antiinflammatory effects. Sprague-Dawley (SD) rats were divided into five groups: normal control group, experimental allergic dermatitis group (vehicle group), low dose of Angelica acutiloba extract (low-dose group), high dose of Angelica acutiloba extract (high-dose group), and antihistamine treated group with experimental dermatitis (antihistamine group). Total leukocyte, neutrophil, lymphocyte, monocyte and eosinophil counts were significantly higher in the vehicle group than in the control group, but these variables were significantly lower in the low- and high-dose groups than in the vehicle group. The platelet/lymphocyte ratio in the red blood cell index was significantly lower in the low- and high-dose groups than in the vehicle group. Low and high doses of the Angelica acutiloba extract did not have toxic effects on liver and kidney. Serum NO, iNOS and levels were highest in the vehicle group but significantly lower in the low- and high-dose groups, especially in the high-dose group. The results of this study suggested that the Angelica acutiloba extract had the effect of alleviating or treating the experimental allergic dermatitis, and it was concluded that the high dose was more effective.

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References

  1. Aggard E. Nitric oxide: mediator, murderer, and medicine. Lancet. 1994. 343: 1199-1206. https://doi.org/10.1016/S0140-6736(94)92405-8
  2. Biliar TR. Nitric oxide: novel biology with clinical relevance. Ann Surg. 1995. 221: 339-349. https://doi.org/10.1097/00000658-199504000-00003
  3. Biyik M, Ucar R, Solak Y. Blood neutrophil-to-lymphocyte ratio independently predicts survival in patients with liver cirrhosis. Eur J Gastroenterol Hepatol. 2013. 25: 435-441. https://doi.org/10.1097/MEG.0b013e32835c2af3
  4. Choy DF, Hsu DK, Seshasayee D. Comparative transcriptomic analyses of atopic dermatitis and psoriasis reveal shared neutrophilicinflammation. J Allergy Clin Immunol. 2012. 130: 1335-1343. https://doi.org/10.1016/j.jaci.2012.06.044
  5. Dogru M, Citli R. The neutrophil-lymphocyte ratio in children with atopic dermatitis: a case-control study. Clin Ter. 2017. 168: 262-265.
  6. Fleisher AB Jr. Treatment of atopic dermatitis: Role of tacrolimus ointment as a topical nonsteroidal therapy. J Alleg Clin Immunol. 1999. 104: 126-130. https://doi.org/10.1016/S0091-6749(99)70055-2
  7. Haper J, Green A, Scott G, Gruendle E, Dorobek B, Cardno M. First experience of topical SDZ ASM 981 in children with atopic dermatitis. Br J Dermatol. 2001, 144: 781-787. https://doi.org/10.1046/j.1365-2133.2001.04133.x
  8. Holden CA, Parish WE. Atopic dermatitis. In: Champion RH, Burton JL, Burns DA, et al., editors. Textbook of dermatology. 6th ed. Oxford: Blackwell Science. 1998. 6: 681-708.
  9. Hong SJ, Korean ISAAC Study Group of Korean Association of Allergy and Respiratory Diseases. Report of Korean ISAAC epidemiologic study for asthma and allergic diseases in children. Pediatr Allergy Respir Dis. 2007. 17: 55-66.
  10. Hon KL, Wang SS, Pong NH. Circulating immunoglobulins, leucocytes and complements in childhood-onset atopic eczema. Indian J Pediatr. 2013. 80: 128-131. https://doi.org/10.1007/s12098-012-0810-0
  11. Imtiaz F, Shafique K, Mirza SS. Neutrophil lymphocyte ratio as a measure of systemic inflammation in prevalent chronic diseases in Asian population. Int Arch Med. 2012. 5: 2. https://doi.org/10.1186/1755-7682-5-2
  12. Kang S, Lucky AW, Praiser D, Lawlence I, Hanifin JM. Long-term safety and efficacy tacrolimus ointment for the treatment of atopic dermatitis in children. J Am Acad Dermatol. 2001. 44: 58-64. https://doi.org/10.1067/mjd.2001.109812
  13. Kayadibi H, Sertoglu E, Uyanik M. Neutrophillymphocyte ratio is useful for the prognosis of patients with hepatocellular carcinoma. World J Gastroenterol. 2014. 20: 9631-9632. https://doi.org/10.3748/wjg.v20.i28.9631
  14. Kim SD, Cho SH. Treatment for atopic dermatitis. J Korean Med Assoc. 2014. 57: 226-233. https://doi.org/10.5124/jkma.2014.57.3.226
  15. Knowles RG, Moncada S. Nitric oxide synthases in mammals. Biochem J. 1994. 298: 249-258. https://doi.org/10.1042/bj2980249
  16. Kolb H, Kolb-Bachofen V. Nitric oxide: a pathogenic factor in autoimmunity. Immunol Today. 1992. 13: 157-160. https://doi.org/10.1016/0167-5699(92)90118-Q
  17. Krakowski AC, Eichenfield LF, Dohil MA. Management of atopic dermatitis in the pediatric population. Pediatrics. 2008. 122: 812-824. https://doi.org/10.1542/peds.2007-2232
  18. Liew FY, Cox FEG. Nonspecific defense mechanism: the role of nitric oxide. Immunol Today. 1991. 12: 17-21. https://doi.org/10.1016/0167-5699(91)90107-5
  19. Lowenstein CJ, Snyder SH. Nitric oxide, a novel biologic messenger. Cell. 1992. 70: 705-707. https://doi.org/10.1016/0092-8674(92)90301-R
  20. Moncada S, Higgs A. The L-arginine-nitric oxide pathway. N Engl J Med. 2012. 329: 2002-2012.
  21. Murphy LA, Atherton DJ. Arzathioprine in severe childhood eczema: value of TPMT as a predictor of outcom and safety in treatment. Br J Dermatol. 2001. 144: 927.
  22. Pawankar R, Bunnag C, Chen Y, Fukuda T, Kim YY, Le LT, Huong le TT, O'Hehir RE, Ohta K, Vichyanond P, Wang DY, Zhong N, Khaltaev N, Bousquet J. Allergic rhinitis and its impact on asthma update (ARIA 2008)-western and Asian-Pacific perspective. Asian Pac J Allergy Immunol. 2009. 27: 237-243.
  23. Sahan E, Polat S. Neutrophil to lymphocyte ratio is associated with more extensive, severe and complex coronary artery disease and impaired myocardial perfusion. Turk Kardiyol Dern Ars. 2014. 42: 415. https://doi.org/10.5543/tkda.2014.87036
  24. Sahin S, Onder M, Sancak B, Bukan N, Gurer MA. The role of nitric oxide in allergic contact dermatitis. Arch Dermatol Res. 2001. 293: 214-217. https://doi.org/10.1007/s004030100207
  25. Sidbury R, Hanifin JM. Systemic therapy of atopic dermatitis. Cli Exp Dermatol. 2000. 25: 559-566. https://doi.org/10.1046/j.1365-2230.2000.00697.x
  26. Spergel JM, Mizoguchi E, Brewer JP. Epicutaneous sensitization with protein antigen induces localized allergic dermatitis and hyperresponsiveness to methacholine after single exposure to aerosolized antigen in mice. J Clin Invest. 1998. 101: 1614-1622. https://doi.org/10.1172/JCI1647
  27. Tao Y, Wang Y, Wang X, Wang C, Bao K, Ji L, Jiang G, Hong M. Calycosin Suppresses Epithelial Derived Initiative Key Factors and Maintains Epithelial Barrier in Allergic Inflammation via TLR4 Mediated NF-${\kappa}B$ Pathway. Cell Physiol Biochem. 2017. 44: 1106-1119. https://doi.org/10.1159/000485416
  28. Uslu AU, Deveci K, Korkmaz S. Is neutrophil/lymphocyte ratio associated with subclinical inflammation and amyloidosis in patients with familial Mediterranean fever? Biomed Res Int. 2013. 185-317.
  29. Vannini F, Kashfi K, Nathb N. The dual role of iNOS in cancer. Redox Biol. 2015. 6: 334-343. https://doi.org/10.1016/j.redox.2015.08.009