Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibitory effects of crude polysaccharide fractions from Annona muricata L. on melanogenesis

그라비올라 잎(Annona muricata L.) 조다당 분획분의 멜라닌 생성 저해 효과

  • Kim, Yi-Eun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University)
  • 김이은 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과)
  • Received : 2018.10.17
  • Accepted : 2018.12.17
  • Published : 2019.02.28
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Abstract

The objective of this study was to evaluate the anti-melanogenic effects of crude polysaccharide fractions from Annona muricata L. (ALP) in 3-isobutyl-1-methylxanthine (IBMX) stimulating hormone-induced mouse B16F10 melanoma cells. The inhibitory effect of ALP on tyrosinase activity was approximately $33.88{\pm}0.79%$ at 5 mg/mL. Additionally, the B16F10 cellular tyrosinase and melanin synthesis inhibition activities by ALP were $54.21{\pm}4.76$ and $56.74{\pm}6.97%$ at $250{\mu}g/mL$, respectively. Similarly, whitening-related protein tyrosinase, tyrosinase-related protein 1 (TRP-1) and TRP-2, and microphthalmia-associated transcription factor (MITF) were reduced by ALP treatment. These results indicated that ALP could be used as a functional cosmetic ingredient after confirming its whitening activity related to melanin content.

본 연구는 그라비올라 잎 조다당 분획물(ALP)의 멜라닌 저해활성에 관하여 알아보기 위하여 B16F10 melanoma 세포의 세포 생존율, tyrosinase 저해활성, 멜라닌 함량, 멜라닌 생합성에 관여하는 단백질(TRP-1, TRP-2, tyrosinase, MITF 및 p-CREB)의 발현을 관찰하였다. ALP의 tyrosinase 활성 저해능을 측정하였을 때, 농도 의존적으로 감소하는 것을 알 수 있었다. 또한 B16F10 세포에 ALP를 처리하여 멜라닌 활성 저해능에 관하여 알아봤을 때, tyrosinase, 멜라닌 함량이 농도 의존적으로 줄어드는 것을 확인하였다. 또한 ALP가 미백과 관련된 TRP-1, TRP-2, tyrosinase, MITF 및 p-CREB의 단백질 발현양이 농도의존적으로 감소되는 것으로 관찰되었다. 따라서 ALP는 멜라닌 저해 활성을 효과적으로 나타낸 것으로 보아 기능성 미백 화장품 소재로서의 활용 가능성을 확인할 수 있었다.

Keywords

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Fig. 2. Cell viability of B16F10 melanoma cells after treating crude polysaccharide fractions from Annona muricata L. (ALP) and IBMX.

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Fig. 3. The difference of melanin contents in B16F10 mouse melanoma cells treated with various solvent fractions of crude polysaccharide fractions from Annona muricata L. (ALP).

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Fig. 4. Effect of crude polysaccharide fractions from Annona muricata L. (ALP) on tyrosinase and melanin synthesis in B16F10 mouse melanoma cells treated with 3-isobutyl-1-methylxanthine (IBMX; 200 μM).

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Fig. 5. Effects crude polysaccharide fractions from Annona muricata L. (ALP) on the levels of protein expression of TRP-1, TRP-2, MITF, Tyrosine, p-CREB in B16F10 mouse melanoma cells.

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Fig. 1. Tyrosinase inhibition activity of crude polysaccharide fractions from Annona muricata L. (ALP).

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