Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on the Improvement Effect of Antibacterial and Allergic Contact Dermatitis on Torreyae Semen Extracts

비자의 항균 및 알레르기성 접촉 피부염 개선 작용 연구

  • Choi, Chan Hun (Department of Korean Medicine Pathology, College of Korean Medicine, Dongeui University) ;
  • Lee, Soong In (Department of Korean Medicine Pathology, College of Korean Medicine, Dongeui University) ;
  • Yim, Soon Ho (Department of Pharmaceutical Engineering, Dongeui University) ;
  • Jung, Hyun Woo (Department of Korean Medicine Pathology, College of Korean Medicine, Dongeui University)
  • Received : 2019.07.16
  • Accepted : 2019.11.15
  • Published : 2019.12.25
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Abstract

The purpose of this study was to investigate the efficacy of Torreyae semen extract against antimicrobial and allergic contact dermatitis to explore its utility as a herbal resource. To this end, the antibacterial effects of Torreyae Semend extracted with hot water and ethanol (30%, 60%, 90%) were investigated using Staphylococcus aureus, Staphylococcus epidermidis, pseudomonas aeruginosa, and Propionibacterium acnes. And observed the anti-atopic dermatitis efficacy with a mouse model that allergic contact dermatitis induced by DNCB. Experimental results showed that the antibacterial activity against S. epidermidis and P. aeruginosa was significantly higher in the hot water extract of Torreyae Semen. We observed that the extracts of 30% ethanol and hot water of Torreyae Semen inhibited growth in a concentration-dependent manner in P. acnes & S. epidermidis. We observed that the administration of hot water extracts and 30% ethanol extract of Torreyae Semen significantly reduce the thickness of ear & back and gross skin condition compared to the control group in the mice with allergic contact dermatitis. As a result of H & E histological staining, it was observed that the Torreyae Semen extraction group had markedly reduced epidermis and skin thickness, granules, and keratinization compared to the control group. The groups administrated with Torreyae Semen hot water extracts had decreased TNF-α and IL-10 contents compared with the control group. As a result, Torreyae Semen may be used as a useful herbal resource because it has a certain level of antibacterial and anti-atopic effects.

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References

  1. Herbology Editorial Committee of Korean Medicine Schools. Herbology. Seoul: Younglimsa; 2012. p. 423-4.
  2. Gao Xuemin. Traditional Chinese Medicine (Volume 1). Beijing : People's Health Publishing House; 2008. p. 956-8.
  3. Seo BI, Jeong KY. Easy-to-understand herbal medicine Revised edition, Daegu: Daegu Haany Univ. Publishing department; 2012. p. 257.
  4. Kim ND. Study on the Anthelmintic Principle of Torreya nucifera, Journal of the Pharmaceutical Society of Korea. 1966;10:30-2.
  5. Jang, SR, Kim DC. In Vitro Anti-bacterial and Anti-inflammatory Effects of Six Types of Herb Aqueous Extracts, J Korean Obstet Gynecol. 2014;27(1):81-100. https://doi.org/10.15204/jkobgy2014.27.1.081
  6. Jeon HS, Lee YS, Kim NW. The Antioxidative Activities of Torreya nucifera Seed Extracts, Journal of the Korean Society of Food Science and Nutrition. 2009;38(1):1-8. https://doi.org/10.3746/JKFN.2009.38.1.001
  7. Kim SB, Kim BW, Hyun SK. Comparison of antioxidant activities and effective compounds in Korean and Chinese Torreya seeds. KOREAN J. FOOD SCI. TECHNOL. 2018;50(3):274-9. https://doi.org/10.9721/KJFST.2018.50.3.274
  8. Lee YS, Jeon HS, Joo EY, Kim NW. Development of Cosmetic Materials Using Torreya nucifera Needles. Journal of Investigative Cosmetology. 2012;8(3):157-63. https://doi.org/10.15810/jic.2012.8.3.001
  9. Son DH, Kim MK, Park DH, Jung ES. Effect of Defatted Torreya nucifera Seed Extract on the Cross-linking of Advanced Glycation End Products to Collagen, J. Soc. Cosmet. Scientists Korea. 2019;45(1):19-25. https://doi.org/10.15230/scsk.2019.45.1.19
  10. http://m.innisfree.com/kr/ko/mProductView.do?prdSeq=13842&cmpid =innisfree-kr-m_mobile:other_kakao:00_five:20170317
  11. Lee JH. Effect of Germinated Brown Rice Enzyme Bread on the Anti-Allergy and Immune, Activity, Professional Graduate School of Oriental Medicine, Wonkwang University, 2010.
  12. Kang SY. Diagnosis and treatment of allergic diseases. Seoul:Ilchkak; 1995. p. 241-52.
  13. Kim SH. A review study concerning treatment of atopic dermatitis in Korean Medicine. Department of PhysiologyGraduate School Daegu Haany University, 2018.
  14. Kim MS, Hwang HI, Lee YR, Kim HW, Park JK. The Effects of Lentinula edodes and Aquilariae agallocha Extracts Combination on the Repair of UVA-Damaged DNA and DNCB-Induced Allergic Dermatitis. The Korean Journal of Food And Nutrition. 2015;28(5):759-65. https://doi.org/10.9799/ksfan.2015.28.5.759
  15. Sim TK, Ko DK, Kim HC, Baek YJ, Lee JS, Yoo HS. Effects of Amyda sinensis on Allergic Inflammation Mechanism related Atopy Dermatitis, Research Institute of Korean Medicine, Taejon University. 2011;20(1):69-83.
  16. Lee JH. Effect of Germinated Brown Rice Enzyme Bread on the Anti-Allergy and Immune, Activity, Professional Graduate School of Oriental Medicine, Wonkwang University, 2010.
  17. Kwang IT. Torreya nucifera Forest on Boolhei Temple in Na-ju, Forest and Culture. 2013;22(4):6-10.
  18. Roh SS, Seo BI, Kim JJ, Park JH. A philological study on poisoning of Torreyae Semen(榧子), The Journal of Applied Oriental Medicine. 2013;13(1):21-4.
  19. Park JE. Screening of physiologically active compounds in extract from Torreya nucifera seed-s shellusing LC-MS/MS, The Graduate School Chung-Ang University, 2015.
  20. Cho CH, Yoo GJ, Kim SY, Lee GS, Kim JH, Hyun JO, et al. Antimicrobial Activity and Cell Cytotoxicity of Korean Plum-yem Extract, Journal of Applied Biological Chemistry, 2009;52(2):45-50. https://doi.org/10.3839/jabc.2009.009
  21. Kim DY, Cho SC. Studies on Anti-inflammatory Effect of Ethanol Extract from Rubus crataegifolius Leaf, Food Eng. Prog. 2016;20(1):21-7. https://doi.org/10.13050/foodengprog.2016.20.1.21
  22. Kim JE, Kim EH, Park SN. Antibaterial Activity of Persicaria hydropiper Extracts and Its Application for Cosmetic Material. Korean Journal of Microbiology and Biotechnology. 2010;38(1):112-5.
  23. Choun YM, Jung JY, Ko KS. A Study on the Possibility of Functional Cosmetic Material by Antioxidant and Antimicrobial Activity of Chelidonium majus Extract. Journal of the Korean Society of Cosmetology. 2017;23(5):921-9.
  24. Lee DS, Kim KH, Yook HS. Cosmetic Effects of the Fractional Extracts from Strawberry (Fragaria ananassa var. 'Seolhyang') Leaf, J. Korean Soc Food Sci Nutr. 2018;47(3):271-8. https://doi.org/10.3746/jkfn.2018.47.3.271
  25. Kim MH. Anti-oxidant and Anti-inflammatory Activities and Contact Dermatitis Treatment Effect of Caffeoyl Quinic Acid Derivatives Isolated from Ilex rotunda, The Graduate School Chung-Ang University, 2013.
  26. Yoo KH. The effects of essential oil of Chamaecyparis Obtuse impregnant fabric for atopic dermatitis, The Graduate School Chung-Ang University, 2015.
  27. Lee SI, Choi CH, Kim JS, Lim SS, Jung HW. The Antioxidant Activities and Neuroprotective Effects of Hot Water Extractsfrom from Torreyae Semen. Kor. J. Herbol. 2017;32(6):41-8. https://doi.org/10.6116/kjh.2017.32.6.41
  28. Ko HJ, Sohn SM, Kim WS, Lee NH, Suh IS. Antimicrobial Activity of Torreya nucifera Extract, International Simposium of the Korean Society for Biotechnology and Bioengineering. 2008:267.
  29. Byun SH, Lee BW, Kim SC. Effect of Samyoyongantang on contact hypersensitivity induced by repeat elicitation of DNCB, Kor. J. Oriental Medic Prescrip. 2005;13:59-69.
  30. Kim CH, Kim KJ. The effects of Yanghyulsamultanggamibang to allergic contact dermatitis, J. Ko.r Oriental Medic Ophthal & Otolaryngol & Dermatol. 2005;18:13-26.
  31. Lim HS. Anti-atopic dermatitis action of Saussurea lappa extract in experimental model, Graduate School Chungnam National University, 2014.
  32. Kim YK, Choi OH, Song CH. Role of IL-10 in the Trimellitic Anhydride-induced Contact Dermatitis, Anatomy & Biological Anthropology. 2013;26(2):71-9.