Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Cryopreservation of in vitro Grown Shoot Tips of Strawberry (Fragaria × ananassa Duch.) Genetic Resources by Droplet-vitrification

  • Received : 2019.09.03
  • Accepted : 2019.12.04
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. 'Wonkyo3114' and 'Gurumi40'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 cm × 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0 mg/L GA3, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA3 for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

Keywords

References

  1. Benelli, C., A. De Carlo and F. Engelmann. 2013. Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol Adv 31:175-185. https://doi.org/10.1016/j.biotechadv.2012.09.004
  2. Clavero-Ramirez, I., J. Galvez-Farfan, J.M. Lopez-Aranda and M.E. Gonzalez-Benito. 2005. Apex cryopreservation of several strawberry genotypes by two encapsulationdehydration methods. Cryo Lett. 26:17-24.
  3. Coste, A., D. Suteu, I. Bacila, C. Deliu, S. Valimareanu and A. Halmagyi. 2015. Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes. Turk. J. Biol. 39:638-648. https://doi.org/10.3906/biy-1411-6
  4. Engelmann, F. 1997. In Biotechnology and Plant Genetic Resources (eds.) Callow JA, Ford-Lloyd BV & Newbury HJ, CAB International. pp. 119-161.
  5. Fahy, G.M., D.R. McFarlene, C.A. Angell and H.T. Meryman. 1984. Vitrification as an approach to cryopreservation. Cryobiology 21:407-426. https://doi.org/10.1016/0011-2240(84)90079-8
  6. Gonzalez-Benito, M.E., I. Clavero-Ramirez and M. Lopez-Aranda. 2004. Review. The use of cryopreservation for germplasm conservation of vegetatively propagated crops. Spanish J. Agri. Res. 2:341-351. https://doi.org/10.5424/sjar/2004023-88
  7. Hirai, D., K. Shirai, S. Shirai and A. Sakai. 1998. Cryopreservation of in vitro grown meristems of strawberry (Fragaria x ananassa Duch) by encapsulation-vitrification. Euphytica 101:109-115. https://doi.org/10.1023/A:1018386211577
  8. Karen, L.C and K.K. Kartha. 2009. Recovery of plants from pea and strawberry meristems cryopreserved for 28 years. Cryo Lett. 30:41-46.
  9. Kim, H.H., E. Popova, D.J. Shin, J.Y. Yi, C.H. Kim, J.S. Lee, M.K. Yoon and F. Engelmann. 2012. Cryobanking of Korean allium germplasm collections: Results from a 10 year experiences. Cryo Lett. 33:45-57.
  10. Kim, H.H., J.G. Lee, D.J. Shin, H.C. Ko, J.G. Gwag, E.G. Cho and F. Engelmann. 2009a. Development of alternative plant vitrification solutions in droplet-vitrification procedures. Cryo Lett. 30:320-334.
  11. Kim, H.H., J.G. Lee, S.U. Park, S.C. Lee, H.J. Baek, E.G. Cho and F. Engelmann. 2009b. Development of alternative loading solutions in droplet-vitrification procedures. Cryo Lett. 30:291-299.
  12. Lambardi, M., A. Fabbri and A. Caccavale. 2000. Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips. Plant Cell Rep. 19:213-218. https://doi.org/10.1007/s002990050001
  13. Lee, Y.G., S.U. Park and H.H. Kim. 2011. Cryopreservation of chrysanthemum shoot tips using the droplet-vitrification technique. CNU J. Agric. Sci. 38:227-233.
  14. Li, B.Q., C.H. Feng, M.R. Wang, L.Y. Hu, G. Volk and Q.C. Wang. 2015. Recovery patterns, histological observations and genetic integrity in Malus shoot tips cryopreserved using droplet-vitrification and encapsulation-dehydration procedures. J. Biotechnol. 214:182-191. https://doi.org/10.1016/j.jbiotec.2015.09.030
  15. Lim, S.H., J.G. Lee, H.J. Lee, K.H. Park, D.S. Kim, S.R. Min, W.S. Jang, T.I. Kim and H.R. Kim. 2016. The genetic diversity among strawberry breeding resources based on SSRs. Sci. Agric. 74:226-234. https://doi.org/10.1590/1678-992X-2016-0046
  16. Medina, J.J., I. Clavero Ramirez, M.E. Gonzalez Benito, J. Galvez Farfan, J.M. Lopez Aranda and C. Soria. 2007. Field performance characterization of strawberry (Fragaria x ananassa Duch.) plants derived from cryopreserved apices. Sci. Hortic. 113:28-32. https://doi.org/10.1016/j.scienta.2007.01.030
  17. Niino, T., D. Tanaka, S. Ichikawa, J. Takano, S. Ivette, K. Shirata and M. Uemura. 2003. Cryopreservation of in vitro-grown apical shoot tips of strawberry by vitrification. Plant Biotechnol. 20:75-80. https://doi.org/10.5511/plantbiotechnology.20.75
  18. Park, S.U and H.H. Kim. 2015. Cryopreservation of sweet potato shoot tips using a droplet-vitrification procedure. Cryo Lett. 36:344-352.
  19. Pinker, I., A. Halmagyi and K. Olbricht. 2009. Effect of sucrose preculture on cryopreservation by droplet-vitrification of strawberry cultivars and morphological stability of cryopreserved plants. Cryo Lett. 30:202-211.
  20. Reed, B.M. 2001. Implementing cryogenic storage of clonally propagated plants. Cryo Lett. 22:97-104.
  21. Reed, B.M. and K.E. Hummer. 1995. Conservation of germplasm of strawberry (Fragaria species): In Biotechnology in Agriculture and Forestry, vol. 32. Cryopreservation of Plant Germplasm I., Bajaj, Y.P.S. (ed.), Springer-Verlag, berlin, Heidelberg, Germany. pp. 354-370.
  22. Rousseau-Gueutin, M., A. Gaston, A. Ainouche, M.L. Ainouche, K. Olbricht, G. Staudt, L. Richard and B. Denoyes-Rothan. 2009. Tracking the evolutionary history of polyploidy in Fragaria L. (strawberry): New insights from phylogenetic analyses of low-copy nuclear genes. Mol. Phylogen. Evol. 51:515-530. https://doi.org/10.1016/j.ympev.2008.12.024
  23. Sakai, A. 2000. In: Cryopreservation of tropical germplasm, (eds.) Engelmann F, Takagi H, JIRCAS, Tsukuba, Japan. pp. 1-7.
  24. Sakai, A. and F. Engelmann. 2007. Vitrification, encapsulation vitrification and droplet-vitrification: a review. Cryo Lett. 28:151-172.
  25. Sakai, A., D. Hirai and T. Niino. 2008. Development of PVS-based vitrification and encapsulation-vitrification protocols: In Reed, B. (ed.), Plant Cryopreservation: A Practical Guide. New York: Springer LLC, USA. pp. 33-58.
  26. Sakai, A., T. Matsumoto, D. Hirai and R. Charoensub. 2002. Survival of tropical apices cooled to-196$^{\circ}C$ by vitrification: In Li, P.H. and E.T. Palva (eds.), Plant Cold Hardiness - Gene Regulation and Genetic Engineering, Kluwer/Plenum, New York, USA. pp. 109-119.
  27. Sant, R., B. Panis, M. Taylor and A. Tyagi. 2008. Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions. Plant Cell Tiss. Org. Cult. 92:107-111. https://doi.org/10.1007/s11240-007-9302-8
  28. Steponkus, P.L., R. Langis and S. Fujikawa. 1992. In: Advances in Low Temperature Biology, (ed.) Steponkus PL, JAI Press, London, UK. pp. 1-61.
  29. Towill, L.E. 1991. Cryopreservation: In Dodds, G.H. (ed.), In Vitro Methods for Conservation of Plant Genetic Resources, Chapman and Hall, Netherlands. pp. 41-71.
  30. Wang, L.Y., Y.D. Li, H.Y. Sun, H.G. Liu, X.D. Tang, Q.C. Wang and Z.D. Zhang. 2017. An efficient dropletvitrification cryopreservation for valuable blueberry germplasm. Sci. Hortic. 219:60-69. https://doi.org/10.1016/j.scienta.2017.03.007
  31. Wang, R.R., X.X. Gao, L. Chen, L.Q. Huo, M.F. Li and Q.C. Wang. 2014. Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification. Sci. Hortic. 176:330-339. https://doi.org/10.1016/j.scienta.2014.07.031
  32. Wang, Q.C., M. Mawassi, P. Li, R. Gafny, I. Selal and E. Tanne. 2003. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci. 165:321-327. https://doi.org/10.1016/S0168-9452(03)00091-8
  33. Wu, Y.J., F. Engelmann, A. Frattarelli, C. Damiano and L.A. Withers. 1997. Cryopreservation of strawberry cell suspension cultures. Cryo Lett. 18:317-324.
  34. Yamamoto, S., T. Rafique, W.S. Priyantha, K. Fukui, T. Matsumoto and T. Niino. 2011. Development of a cryopreservation procedure using aluminium cryo-plates. Cryo Lett. 32:256-265.
  35. Yamamoto, S., K. Fukui, T. Rafique, N.I. Khan, M. Castillo, R. Carlos, K. Sekizawa, T. Matsumoto and T. Niino. 2012. Cryopreservation of in vitro-grown shoot tips of strawberry by the vitrification method using aluminum cryo-plates. Plant Gen. Res. 10:14-19. https://doi.org/10.1017/S1479262111000906
  36. Yi, J.Y., K. Balaraju, H.J. Baek, M.S. Yoon, H.H. Kim and Y.Y. Lee. 2018a. A successful regeneration from shoot tips of Chrysanthemum morifolium (Ramat.) following cryopreservation by droplet-vitrification. Korean J. Plant Res. 31:675- 683. https://doi.org/10.7732/kjpr.2018.31.6.675
  37. Yi, J.Y., K. Balaraju, H.J. Baek, M.S. Yoon, H.H. Kim and Y.Y. Lee. 2018b. Cryopreservation of Citrus limon (L.) Burm. F shoot tips using a droplet-vitrification method. Korean J. Plant Res. 31:684-694. https://doi.org/10.7732/kjpr.2018.31.6.684
  38. Zhang, J.M., L. Han, X.X. Lu, G.M. Volk, X. Xin, G.K. Yin, J.J. He, L. Wang and X.L. Chen. 2017. Cryopreservation of Jerusalem artichoke cultivars using an improved dropletvitrification method. Plant Cell Tiss. Org. Cult. 128:577-587. https://doi.org/10.1007/s11240-016-1135-x