한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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유전자변형 미세조류의 생태 유출 모니터링 및 위해성평가 연구

Monitoring and Environmental Risk Assessment of Genetically Modified Microalgae

  • 조기철 (국립해양생물자원관 유전자원연구실) ;
  • 전한철 (국립해양생물자원관 유전자원연구실) ;
  • 황현주 (국립해양생물자원관 유전자원연구실) ;
  • 홍지원 (국립해양생물자원관 분류연구실) ;
  • 이대성 (국립해양생물자원관 유전자원연구실) ;
  • 한종원 (국립해양생물자원관 유전자원연구실)
  • Cho, Kichul (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Jeon, Hancheol (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Hwang, Hyun-Ju (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Hong, Ji Won (Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) ;
  • Lee, Dae-Sung (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Han, Jong Won (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea)
  • 투고 : 2019.11.20
  • 심사 : 2019.11.28
  • 발행 : 2019.12.31
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초록

Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.

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