Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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A Novel Endo-β-1,4-xylanase from Acanthophysium sp. KMF001, a Wood Rotting Fungus

  • Yoon, Sae-Min (Department of Forest Products and Biotechnology, Kookmin University) ;
  • Kim, Yeong-Suk (Department of Forest Products and Biotechnology, Kookmin University) ;
  • Kim, Young-Kyoon (Department of Forest Products and Biotechnology, Kookmin University) ;
  • Kim, Tae-Jong (Department of Forest Products and Biotechnology, Kookmin University)
  • Received : 2018.07.10
  • Accepted : 2018.10.18
  • Published : 2018.11.25
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Abstract

Acanthophysium sp. KMF001, a wood rotting fungus, produces a strong crude enzyme complex that efficiently produces simple sugars from wood. The transcriptomic analysis of Acanthophysium sp. KMF001 identified 14 genes for putative glycoside hydrolases. Among them, isotig01043 was expressed heterogeneously in Escherichia coli BL21(DE3), and the expressed protein exhibited an endo-${\beta}$-1,4-xylanase activity which showed the optimum reaction at pH 5.0 and $30^{\circ}C$. The enzyme kinetic values of $K_m$ and $V_{max}$ were 25.92 mg/ml and $0.628{\mu}mole/mg/ml$, respectively. The enzymatic characteristics of the expressed xylanase showed a typical fungal xylanase. However, the bioinformatics analysis suggested that the protein encoded by isotig01043 was a novel xylanase based on a low identity when it was compared with the closest protein in the NCBI database and a similar protein domain with GH16_fungal_Lam16A_glucanase, which had not been earlier suggested as a xylanase.

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References

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