Mycobiology

The Korean Society of Mycology (한국균학회)
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LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast

  • Park, Yoon-Dong (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Kwon, Soo Jeong (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Bae, Kyung Sook (MicroID Co. Ltd) ;
  • Park, Hee-Moon (Department of Microbiology & Molecular Biology, Chungnam National University)
  • Received : 2018.05.18
  • Accepted : 2018.08.03
  • Published : 2018.09.01
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Abstract

The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of $lkh1^+$, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of $prk1^+$, another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactosespecific flocculation. Deletion of prk1 increased the flocculation phenotype of the $lkh1^+$ null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of $prk1^+$ were also decreased by $lkh1^+$ deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.

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References

  1. Stratford M. Yeast flocculation: a new perspective. Adv Microb Physiol. 1992;33:2-71.
  2. Straver MH, Kijne JW, Smit G. Cause and control of flocculation in yeast. Trends Biotechnol. 1993;11:228-232. https://doi.org/10.1016/0167-7799(93)90133-T
  3. Hope EA, Amorosi CJ, Miller AW, et al. Experimental evolution reveals favored adaptive routes to cell aggregation in yeast. Genetics. 2017;206:1153-1167. https://doi.org/10.1534/genetics.116.198895
  4. Miki BL, Poon NH, James AP, et al. Possible mechanism for flocculation interactions governed by gene FLO1 in Saccharomyces cerevisiae. J Bacteriol. 1982;150:878-889.
  5. Shankar CS, Umesh-Kumar S. A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae. Microbiology. 1994;140:1097-1101. https://doi.org/10.1099/13500872-140-5-1097
  6. Yamashita I, Fukui S. Mating signals control expression of both starch fermentation genes and a novel flocculation gene FLOSin the yeast Saccharomyces. Agric Biol Chem. 1983;47:2889-2896.
  7. Stratford M, Assinder S. Yeast flocculation: Flo1 and NewFlo phenotypes and receptor structure. Yeast. 1991;7:559-574. https://doi.org/10.1002/yea.320070604
  8. Teunissen AW, Steensma HY. Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family. Yeast. 1995;11:1001-1013. https://doi.org/10.1002/yea.320111102
  9. Lo WS, Dranginis AM. FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin. J Bacteriol. 1996;178:7144-7151. https://doi.org/10.1128/jb.178.24.7144-7151.1996
  10. Sieiro C, Reboredo NM, Blanco P, et al. Cloning of a new FLO gene from the flocculating Saccharomyces cerevisiae IM1-8b strain. FEMS Microbiol Lett. 1997;146:109-115. https://doi.org/10.1111/j.1574-6968.1997.tb10179.x
  11. Bossier P, Goethals P, Rodrigues-Pousada C. Constitutive flocculation in Saccharomyces cerevisiae through overexpression of the GTS1 gene, coding for a 'Glo'-type Zn-finger-containing protein. Yeast. 1997;13:717-725. https://doi.org/10.1002/(SICI)1097-0061(19970630)13:8<717::AID-YEA132>3.0.CO;2-2
  12. Di Gianvito P, Tesniere C, Suzzi G, et al. FLO5 gene controls flocculation phenotype and adhesive properties in a Saccharomyces cerevisiae sparkling wine strain. Sci Rep. 2017;7:10786. https://doi.org/10.1038/s41598-017-09990-9
  13. Watson P, Davey J. Characterization of the Prk1 protein kinase from Schizosaccharomyces pombe. Yeast. 1998;14:485-492. https://doi.org/10.1002/(SICI)1097-0061(19980330)14:5<485::AID-YEA239>3.0.CO;2-V
  14. Kim KH, Cho YM, Kang WH, et al. Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog. Biochem Biophys Res Commun. 2001;289:1237-1242. https://doi.org/10.1006/bbrc.2001.6128
  15. Kang WH, Park YH, Park HM. The LAMMER kinase homolog, Lkh1, regulates Tup transcriptional repressors through phosphorylation in Schizosaccharomyces pombe. J Biol Chem. 2010;285:13797-13806. https://doi.org/10.1074/jbc.M110.113555
  16. Liu Z, Li R, Dong Q, et al. Characterization of the non-sexual flocculation of fission yeast cells that results from the deletion of ribosomal protein L32. Yeast. 2015;32:439-449. https://doi.org/10.1002/yea.3070
  17. Li R, Li X, Sun L, et al. Reduction of ribosome level triggers flocculation of fission yeast cells. Eukaryotic Cell . 2013;12:450-459. https://doi.org/10.1128/EC.00321-12
  18. Matsuzawa T, Morita T, Tanaka N, et al. Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe. Mol Microbiol. 2011;82:1531-1544. https://doi.org/10.1111/j.1365-2958.2011.07908.x
  19. Moreno S, Klar A, Nurse P. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 1991;194:795-823.
  20. Alfa C, Fantes P, Hyams J, et al. Cold Spring Harbor Laboratory. Experiments with fission yeast: a laboratory course manual. New York: Cold Spring Harbor Laboratory Press; 1993.
  21. Hengen PN. Methods and reagents. preparing ultra-competent Escherichia coli. Trends Biochem Sci. 1996;21:75-76.
  22. Maundrell K. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene. 1993;123:127-130. https://doi.org/10.1016/0378-1119(93)90551-D
  23. Grimm C, Kohli J, Murray J, et al. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet. 1988;215:81-86. https://doi.org/10.1007/BF00331307
  24. Okazaki K, Okazaki N, Kume K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 1990;18:6485-6489. https://doi.org/10.1093/nar/18.22.6485
  25. Bellal M, Boudrant J, Elfoul L, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 1995;30:641-648. https://doi.org/10.1016/0032-9592(94)00046-8
  26. Su Y, Chen J, Huang Y. Disruption of ppr3, ppr4, ppr6, or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe. FEMS Microbiol Lett. 2018;365:16.
  27. Tanaka N, Awai A, Bhuiyan MS, et al. Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe. J Bacteriol. 1999;181:1356-1359.

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