Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of 5α-reductase of de novo Generation of Short Anti-oxidant Peptides

  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • Received : 2018.07.11
  • Accepted : 2018.10.01
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study aims to investigate the biological activities related to hair loss of short anti-oxidant peptides (DK peptides) $5{\alpha}$-reductase inhibition and anti-oxidation. The series of DK peptides were generated amphipathic helical properties using leucines, lysines and tryptophan residues. Cell viability and free radical scavenging activities were performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The DK peptides were investigated for inhibitory activity against $5{\alpha}$-reductase. Antioxidant activities were determined by means of, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. All peptides could inhibit $5{\alpha}$-reductase in lipopolysaccharide-stimulated macrophage. In conclusion, DK peptides was suggested as the most attractive ingredients for improving hair loss, because of the high inhibitory against $5{\alpha}$-reductase inhibition.

Keywords

References

  1. Ahn SY, Pi LQ, Hwang ST, Lee WS. Effect of IGF-I on hair growth is related to the anti-apoptotic effect of IGF-I and up-regulation of PDGF-A and PDGF-B. Annals of Dermatology. 2012. 24: 26-31. https://doi.org/10.5021/ad.2012.24.1.26
  2. Amory JK, Wang C, Swerdloff RS, Anawalt BD, Matsumoto AM, Bremner WJ, Walker SE, Haberer LJ, Clark RV. The effect of 5alpha-reductase inhibition with dutasteride and finasteride on semen parameters and serum hormones in healthy men. J Clin Endocrinol Metab. 2007. 92: 1659-1665. https://doi.org/10.1210/jc.2006-2203
  3. Cotsarelis G, Millar SE. Towards a molecular understanding of hair loss and its treatment. Trends in Molecular Medicine. 2001. 7: 293-301.
  4. Dawber RPR. Aetiology and pathophysiology of hair loss. Dermatology. 1987. 175: 23-28.
  5. Dmytriw AA, Morzycki W, Green PJ. Prevention of Alopecia in Medical and Interventional Chemotherapy Patients. J Cutan Med Surg. 2015. 19: 11-16. https://doi.org/10.2310/7750.2014.13200
  6. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrite in biological fluids. Analytical Biochemistry. 1982. 126: 131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  7. Huang KP, Mullangi S, Guo Y, Qureshi AA. Autoimmune, atopic, and mental health comorbid conditions associated with alopecia areata in the United States. JAMA Dermatol. 2013. 149: 789-794. https://doi.org/10.1001/jamadermatol.2013.3049
  8. Nanjo F, Goto K, Seto R, Suzuki M. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biology And Medicine. 1996. 21: 895-902. https://doi.org/10.1016/0891-5849(96)00237-7
  9. Kwack MH, Ahn JS, Kim MK, Kim JC, Sung, YK. Dihydrotestosterone-inducible IL-6 inhibits elongation of human hair shafts by suppressing matrix cell proliferation and promotes regression of hair follicles in mice. Journal of Investigative Dermatology. 2012. 132: 43-49. https://doi.org/10.1038/jid.2011.274
  10. Matsuda H, Yamazaki M, Matsuo K, Asanuma Y, Kubo M. Antiandrogenic Activity of Myricae Cortex-Isolation of Active Constituents from Bark of Myrica rubra. Biological and Pharmaceutical Bulletin. 2001. 24: 259-263. https://doi.org/10.1248/bpb.24.259
  11. Naito A, Midorikawa T, Yoshino T, Ohdera M. Lipid peroxides induce early onset of catagen phase in murine hair cycles. International Journal of Molecular Medicine. 2008. 22: 725.
  12. Otberg N, Finner AM, Shapiro J. Androgenetic alopecia. Endocrinology Metabolism Clinics of North America. 2007. 36: 379 -398. https://doi.org/10.1016/j.ecl.2007.03.004
  13. Paus R, Cotsarelis G. The biology of hair follicles. N Engl J Med 1999. 341: 491-497. https://doi.org/10.1056/NEJM199908123410706
  14. Stough D, Stenn K, Haber R, Parsley WM, Vogel JE, Whiting DA. Washenik, K. Psychological effect, pathophysiology, and management of androgenetic alopecia in men. Mayo Clinic Proceedings. 2005. 80: 1316-1322. https://doi.org/10.4065/80.10.1316
  15. Traish AM, Hassani J, Guay AT, Zitzmann M, Hansen ML. Adverse side effects of 5alpha-reductase inhibitors therapy: persistent diminished libido and erectile dysfunction and depression in a subset of patients. J Sex Med. 2011. 8: 872-884. https://doi.org/10.1111/j.1743-6109.2010.02157.x
  16. Yim E, Nole KL, Tosti A. 5alpha-Reductase inhibitors in androgenetic alopecia. Curr Opin Endocrinol Diabetes Obes. 2014. 21:493-498. https://doi.org/10.1097/MED.0000000000000112
  17. Whiting DA. Possible mechanisms of miniaturization during androgenetic alopecia or pattern hair loss. Journal of the American Academy of Dermatology. 2001. 45: 81-86. https://doi.org/10.1067/mjd.2001.117428
  18. Wood JM, Decker H, Hartmann H, Chavan B, Rokos H, Spencer JD, Hasse S, Thornton MJ, Shalbaf M, Paus R. Senile hair graying: $H_{2}O_{2}$-mediated oxidative stress affects human hair color by blunting methionine sulfoxide repair. The FASEB Journal. 2009. 23: 2065-2075. https://doi.org/10.1096/fj.08-125435