Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Efficient Isolation and Characterization of a Cellulase Hyperproducing Mutant Strain of Trichoderma reesei

  • Zou, Zongsheng (National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University) ;
  • Zhao, Yunying (National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University) ;
  • Zhang, Tingzhou (Zhejiang Cofine Biotech. Inc.) ;
  • Xu, Jiaxing (Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University) ;
  • He, Aiyong (Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University) ;
  • Deng, Yu (National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University)
  • Received : 2018.05.09
  • Accepted : 2018.07.27
  • Published : 2018.09.28
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Abstract

A cellulase hyperproducing mutant strain, JNDY-13, was obtained using the ARTP mutation system and with Trichoderma reesei RUT-C30 as the parent strain. Whole-genome sequencing of JNDY-13 confirmed that 105 of the 653 SNPs were point mutations, 336 mutations were deletions and 165 were insertions. Moreover, 99 mutations were insertions and duplications. Among all the mutations, the one that occurred in the galactokinase gene might be related to the production of cellulases in T. reesei JNDY-13. Moreover, the up-regulation of cellulase and hemicellulase genes in JNDY-13 might contribute to higher cellulases production. Under optimal conditions, the highest cellulase activity by batch fermentation reached 4.35 U/ml, and the highest activity of fed-batch fermentation achieved was 5.40 U/ml.

Keywords

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