Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Enhancement of Tomato Tolerance to Biotic and Abiotic Stresses by Variovorax sp. PMC12

Variovorax sp. PMC12 균주에 의한 토마토의 생물학 및 비생물학적 스트레스 저항성 증진

  • Kim, Hyeon Su (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Shin Ae (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Yiseul (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sang, Mee kyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Song, Jaekyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Chae, Jong-Chan (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Weon, Hang-Yeon (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • 김현수 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 이신애 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 김이슬 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 상미경 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 송재경 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과) ;
  • 채종찬 (전북대학교 환경생명자원대학 생명공학부) ;
  • 원항연 (농촌진흥청 국립농업과학원 농업생물부 농업미생물과)
  • Received : 2018.08.10
  • Accepted : 2018.09.02
  • Published : 2018.09.30
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Abstract

Rhizobacteria play important roles in plant growth and health enhancement and render them resistant to not only biotic stresses but also abiotic stresses, such as low/high temperature, drought, and salinity. This study aimed to select plant growth promoting rhizobacteria (PGPR) with the capability to mitigate biotic and abiotic stress effects on tomato plants. We isolated a novel PGPR strain, Variovorax sp. PMC12 from tomato rhizosphere. An in vitro assay indicated that strain PMC12 produced ammonia, indole-3-acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which are well-known traits of PGPR. The aboveground fresh weight was significantly higher in tomato plants treated with strain PMC12 than in non-treated tomato plants under various abiotic stress conditions including salinity, low temperature, and drought. Furthermore, strain PMC12 also enhanced the resistance to bacterial wilt disease caused by Ralstonia solanacearum. Taken together, these results indicated that strain PMC12 is a promising biocontrol agent and a biostimulant to reduce the susceptibility of plants to both abiotic and biotic stresses.

근권세균은 식물 생육과 건강 증진에 중요한 역할을 하며 생물학적 스트레스뿐만 아니라 저온, 고온, 건조 및 염과 같은 비생물적 스트레스에도 내성을 부여한다. 본 연구는 토마토에 생물적 및 비생물적 스트레스를 완화시키는 기능을 가진 식물생장촉진 근권세균(plant growth promoting rhizobacteria, PGPR)을 선발하는 것을 목표로 하였으며 토마토 근권에서 Variovorax sp. PMC12균주를 분리하였다. PMC12균주는 in vitro에서 PGPR의 특성으로 알려진 암모니아, IAA, 시드로포아 및 ACC 탈아민효소를 생성하였다. PMC12 균주를 처리한 토마토는 대조구에 비해 염, 저온 및 건조 스트레스 조건에서 지상부 생체중이 유의적으로 높았다. 또한 PMC12 균주를 처리한 토마토는 Ralstonia solanacearum에 의한 세균성 시들음병에 대한 저항성이 증가되었다. 결과적으로 PMC12 균주는 식물의 비생물적 스트레스 및 생물적 스트레스에 대한 감수성을 감소시키는 유망한 생물학적 방제제 및 생물활성제로 사용될 수 있을 것으로 전망된다.

Keywords

References

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