한국축산식품학회지 (Food Science of Animal Resources)

  • 제38권4호
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  • Pages.703-710
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  • 2018
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Identification of a Bromodomain-containing Protein 2 (BRD2) Gene Polymorphic Variant and Its Effects on Pork Quality Traits in Berkshire Pigs

  • Lee, Dong Ju (Department of Animal Resource Technology, Gyeongnam National University of Science & Technology) ;
  • Hwang, Jung Hye (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Ha, Jeongim (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Yu, Go Eun (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Kwon, Seulgi (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Park, Da Hye (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Kang, Deok Gyeong (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Kim, Tae Wan (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Park, Hwa Chun (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • An, Sang Mi (Swine Science and Technology Center, Gyeongnam National University of Science & Technology) ;
  • Kim, Chul Wook (Department of Animal Resource Technology, Gyeongnam National University of Science & Technology)
  • 투고 : 2017.11.27
  • 심사 : 2018.06.14
  • 발행 : 2018.08.31
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초록

Bromodomain-containing protein 2 (BRD2) is a nuclear serine/threonine kinase involved in transcriptional regulation. We investigated the expression and association of the BRD2 gene as a candidate gene for meat quality traits in Berkshire pigs. BRD2 mRNA was expressed at relatively high levels in muscle tissue. Statistical analysis revealed that the c.1709G>C polymorphism of the BRD2 gene was significantly associated with carcass weight, meat color ($a^*$, redness), protein content, cooking loss, water-holding capacity, carcass temperatures 4, 12 and 24 h postmortem, and the 24 h postmortem pH in 384 Berkshire pigs. Therefore, this polymorphism in the porcine BRD2 gene may be used as a candidate genetic marker to improve meat quality traits in pigs.

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참고문헌

  1. Baby S, Hyeong KE, Lee YM, Jung JH, Oh DY, Nam KC, Kim TH, Lee HK, Kim JJ. 2014. Evaluation of genome based estimated breeding values for meat quality in a Berkshire population using high density single nucleotide polymorphism chips. Asian-Australas J Anim Sci 27:1540-1547. https://doi.org/10.5713/ajas.2014.14371
  2. Belkina AC, Nikolajczyk BS, Denis GV. 2013. BET protein function is required for inflammation: BRD2 genetic disruption and BET inhibitor JQ1 impair mouse macrophage inflammatory responses. J Immunol 190:3670-3678. https://doi.org/10.4049/jimmunol.1202838
  3. Bourne G. 1973. The structure and function of muscle: Structure, part II. Academic Press, Inc., New York, NY, USA.
  4. Casiro S, Velez-Irizarry D, Ernst CW, Raney NE, Bates RO, Charles MG, Steibel JP. 2017. Genome-wide association study in an F2 Duroc${\times}$Pietrain resource population for economically important meat quality and carcass traits. J Anim Sci 95:545-558.
  5. Denis GV, Green MR. 1996. A novel, mitogen-activated nuclear kinase is related to a Drosophila developmental regulator. Genes Dev 10:261-271. https://doi.org/10.1101/gad.10.3.261
  6. Devine C, Dikeman M. 2014. Encyclopedia of meat sciences. 2nd ed. Elsevier, New York, NY, USA.
  7. Falconer DS, Mackay TFC. 1996. Introduction to quantitative genetics. 4th ed. Longman, Harlow, UK.
  8. Fan B, Lkhagvadorj S, Cai W, Young J, Smith RM, Dekkers JCM, Huff-Lonergan E, Lonergan SM, Rothschild MF. 2010. Identification of genetic markers associated with residual feed intake and meat quality traits in the pig. Meat Sci 84:645-650. https://doi.org/10.1016/j.meatsci.2009.10.025
  9. Handschin C, Spiegelman BM. 2006. Peroxisome proliferator-activated receptor gamma coactivator 1 coactivators, energy homeostasis, and metabolism. Endocr Rev 27:728-735. https://doi.org/10.1210/er.2006-0037
  10. Horwitz W. 2000. Official methods of analysis of AOAC International. 17th ed. Association of Official Analytical Chemists. Gaithersburg, MD, USA.
  11. Hwang JH, An SM, Kwon SG, Park DH, Kim TW, Kang DG, Yu GE, Kim IS, Park HC, Ha J, Kim CW. 2017. Associations of the polymorphisms in DHRS4, SERPING1, and APOR genes with postmortem pH in Berkshire pigs. Anim biotechnol 28:288-293. https://doi.org/10.1080/10495398.2017.1279171
  12. Jung WY, Kwon SG, Son M, Cho ES, Lee Y, Kim JH, Kim BW, Park DH, Hwang JH, Kim TW, Park HC, Park BY, Choi JS, Cho KK, Chung KH, Song YM, Kim IS, Jin SK, Kim DH, Lee SW, Lee KW, Bang WY, Kim CW. 2012. RNA-Seq approach for genetic improvement of meat quality in pig and evolutionary insight into the substrate specificity of animal carbonyl reductases. Plos One 7:e42198. https://doi.org/10.1371/journal.pone.0042198
  13. Kanno T, Kanno Y, Siegel RM, Jang MK, Lenardo MJ, Ozato K. 2004. Selective recognition of acetylated histones by bromodomain proteins visualized in living cells. Mol Cell 13:33-43. https://doi.org/10.1016/S1097-2765(03)00482-9
  14. Kwon SG, Hwang JH, Park DH, Kim TW, Kang DG, Kang KH, Kim IS, Ha J, Kim CW. 2016. Effects of a non-synonymous CBG gene single nucleotide polymorphism (SNP) on meat-quality traits in Berkshire pigs. Can J Anim Sci 96:45-51. https://doi.org/10.1139/cjas-2015-0074
  15. Maribo H, Olsen EV, Barton-Gade P, Moller AJ, Karlsson A. 1998. Effect of early post-mortem cooling on temperature, pH fall and meat quality in pigs. Meat Sci 50:115-129. https://doi.org/10.1016/S0309-1740(98)00022-9
  16. Michaeloudes C, Mercado N, Clarke C, Bhavsar PK, Adcock IM, Barnes PJ, Chung KF. 2014. Bromodomain and extraterminal proteins suppress NF-E2-related factor 2-mediated antioxidant gene expression. J Immunol 192:4913-4920. https://doi.org/10.4049/jimmunol.1301984
  17. Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics methods and protocols. Misener S, Krawetz SA (ed). Humana Press, Totowa, NJ, USA. pp 365-386.
  18. Shang E, Cui Q, Wang X, Beseler C, Greenberg DA, Wolgemuth DJ. 2011. The bromodomain-containing gene BRD2 is regulated at transcription, splicing, and translation levels. J Cell Biochem 112:2784-2793. https://doi.org/10.1002/jcb.23192
  19. Szabo F, Bokor A, Bene S, Polgar JP. 2011. Animal breeding. Pannon Univ., Kaposvar Univ., Hungary.
  20. Taniguchi Y, Matsuzaka Y, Fujimoto H, Miyado K, Kohda A, Okumura K, Kimura M, Inoko H. 1998. Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells. Genomics 51:114-123. https://doi.org/10.1006/geno.1998.5262
  21. Thorpe KL, Beck S. 1998. DNA sequence and structure of the mouse RING3 gene: Identification of variant RING3 transcripts. Immunogenetics 48:82-86. https://doi.org/10.1007/s002510050406
  22. Van der Steen HAM, Prall GFW, Plastow GS. 2005. Application of genomics to the pork industry. J Anim Sci 83:E1-E8. https://doi.org/10.2527/2005.8311
  23. Wang F, Deeney JT, Denis GV. 2013. Brd2 gene disruption causes "metabolically healthy" obesity: Epigenetic and chromatin-based mechanisms that uncouple obesity from type 2 diabetes. Vitam Horm 91:49-75.
  24. Wang F, Liu H, Blanton WP, Belkina A, Lebrasseur NK, Denis GV. 2009. Brd2 disruption in mice causes severe obesity without Type 2 diabetes. Biochem J 425:71-83.
  25. Zang K, Wang J, Dong M, Sun R, Wang Y, Huang Y, Liu X, Li Y, Wang F, Yu M. 2013. Brd2 inhibits adipogenesis via the ERK1/2 signaling pathway in 3T3-L1 adipocytes. Plos One 8:e78536. https://doi.org/10.1371/journal.pone.0078536

피인용 문헌

  1. The BET family in immunity and disease vol.6, pp.1, 2018, https://doi.org/10.1038/s41392-020-00384-4