Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

  • Chaijak, Pimprapa (Department of Biotechnology, Faculty of Science, Thaksin University, Phatthalung Campus) ;
  • Sukkasem, Chontisa (Department of Food Science and Technology, Faculty of Technology and Community Development, Thaksin University, Phatthalung Campus) ;
  • Lertworapreecha, Monthon (Department of Biology, Faculty of Science, Thaksin University, Phatthalung Campus) ;
  • Boonsawang, Piyarat (Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hatyai Campus) ;
  • Wijasika, Sutthida (Department of Biotechnology, Faculty of Technology and Community Development, Thaksin University, Phatthalung Campus) ;
  • Sato, Chikashi (Department of Civil and Environmental Engineering, College of Science and Engineering, Idaho State University)
  • Received : 2018.03.13
  • Accepted : 2018.06.11
  • Published : 2018.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

The fungi associated with termites secrete enzymes such as laccase (multi-copper oxidase) that can degrade extracellular wood matrix. Laccase uses molecular oxygen as an electron acceptor to catalyze the degradation of organic compounds. Owing to its ability to transfer electrons from the cathodic electrode to molecular oxygen, laccase has the potential to be a biocatalyst on the surface of the cathodic electrode of a microbial fuel cell (MFC). In this study, a two-chamber MFC using the laccase-producing fungus Galactomyces reessii was investigated. The fungus cultured on coconut coir was placed in the cathode chamber, while an anaerobic microbial community was maintained in the anode chamber fed by industrial rubber wastewater and supplemented by sulfate and a pH buffer. The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a $1,000{\Omega}$ resistor), power density of $59mW/m^2$, and current density of $278mA/m^2$, and a 70% increase in half-cell potential. This study demonstrated the capability of laccase-producing yeast Galactomyces reessii as a biocatalyst on the cathode of the two-chamber lbMFC.

Keywords

References

  1. Logan BE, Rabaey K. 2012. Conversion of wastes into bioelectricity and chemicals by using microbial electrochemical technologies. Science 337: 686-690. https://doi.org/10.1126/science.1217412
  2. Zhang F, Chen M, Zhang Y, Zeng RJ. 2012. Microbial desalination cells with ion exchange resin packed to enhance desalination at low salt concentration. J. Memb. Sci. 417: 28-33.
  3. Sukkasem C, Laehlah S. 2013. Development of a UBFC biocatalyst fuel cell to generate power and treat industrial wastewaters. Bioresour. Technol. 146: 749-753. https://doi.org/10.1016/j.biortech.2013.07.065
  4. Pozdnyakova NN, Rodakiewicz-Nowak J, Turkovskaya OV, Haber J. 2006. Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators. Enzyme Microb. Technol. 39: 1242-1249. https://doi.org/10.1016/j.enzmictec.2006.03.009
  5. Lee H, Jang Y, Choi YS, Kim MJ, Lee J, Lee H, et al. 2014. Biotechnological procedures to select white rot fungi for the degradation of PAHs. J. Microbiol. Methods 97: 56-62. https://doi.org/10.1016/j.mimet.2013.12.007
  6. Barton SC, Pickard M, Vazquez-Duhalt R, Heller A. 2002. Electroreduction of O-2 to water at 0.6 V (SHE) at pH 7 on the 'wired' Pleurotus ostreatus laccase cathode. Biosens. Bioelectron. 17: 1071-1074. https://doi.org/10.1016/S0956-5663(02)00100-8
  7. Fishilevich S, Amir L, Fridman Y, Aharoni A, Alfonta L. 2009. Surface display of redox enzymes in microbial fuel cells. J. Am. Chem. Soc. 131: 12052-12053. https://doi.org/10.1021/ja9042017
  8. Wu C, Liu XW, Li WW, Sheng GP, Zang GL, Cheng YY, et al. 2012. A white-rot fungus is used as a biocathode to improve electricity production of a microbial fuel cell. Appl. Energy 98: 594-596. https://doi.org/10.1016/j.apenergy.2012.02.058
  9. Lai CY, Wu CH, Meng CT, Lin CW. 2017. Decolorization of azo dye and generation of electricity by microbial fuel cell with laccase-producing white-rot fungus on cathode. Appl. Energy 188: 392-398. https://doi.org/10.1016/j.apenergy.2016.12.044
  10. Mani P, Keshavarz T, Chandra TS, Kyazze G. 2017. Decolourisation of acid orange 7 in a microbial fuel cell with a laccase-based biocathode: influence of mitigating pH changes in the cathode chamber. Enzyme Microb. Technol. 96: 170-176. https://doi.org/10.1016/j.enzmictec.2016.10.012
  11. Chaijak P, Lertworapreecha M, Sukkasem C. 2018. Phenol removal from palm oil mill effluent using Galactomyces Reessii termite-associated yeast. Pol. J. Environ Stud. 27: 39-44. https://doi.org/10.15244/pjoes/75205
  12. Muyzer G, Dewaal EC, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S ribosomal RNA. Appl. Environ. Microbiol. 59: 695-700.
  13. de Lillo A, Ashley FP, Palmer RM, Munson MA, Kyriacou L, Weightman AJ, et al. 2006. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol. Immunol. 21: 61-68. https://doi.org/10.1111/j.1399-302X.2005.00255.x
  14. Mohammadi M, Man HC, Hassan MA, Yee PL. 2010. Treatment of wastewater from rubber industry in Malaysia. Afr. J. Biotechnol. 9: 6233-6243.
  15. Lai CY, Liu SH, Wu GP, Lin CW. 2017. Enhanced biodecolorization of acid orange 7 and electricity generation in microbial fuel cells with superabsorbent-containing membrane and laccase-based bio-cathode. J. Clean. Prod. 166: 381-386. https://doi.org/10.1016/j.jclepro.2017.08.047
  16. Chu BTT, Petrovich ML, Chaudhary A, Wright D, Murphy B, Wells G, et al. 2018. Metagenomics reveals the impact of wastewater treatment plants on the dispersal of microorganisms and genes in aquatic sediments. Appl. Environ. Microbiol. 84: e02168-17.
  17. Sun HH, Yu P, Li QL, Ren HQ, Liu B, Ye L, Zhang XX. 2017. Transformation of anaerobic granules into aerobic granules and the succession of bacterial community. Appl. Microbiol. Biotechnol. 101: 7703-7713. https://doi.org/10.1007/s00253-017-8491-2
  18. Oh KH, Lee SY, Lee MH, Oh TK, Yoon JH. 2011. Paraperlucidibaca baekdonensis gen. nov., sp nov., isolated from seawater. Int. J. Syst. Evol. Microbiol. 61: 1382-1385. https://doi.org/10.1099/ijs.0.023994-0
  19. Martins MD, Rigonato J, Taboga SR, Branco LHZ. 2016. Proposal of Ancylothrix gen. nov., a new genus of Phormidiaceae (Cyanobacteria, Oscillatoriales) based on a polyphasic approach. Int. J. Syst. Evol. Microbiol. 66: 2396-2405. https://doi.org/10.1099/ijsem.0.001044
  20. Tanikawa D, Syutsubo K, Hatamoto M, Fukuda M, Takahashi M, Choeisai PK, et al. 2016. Treatment of natural rubber processing wastewater using a combination system of a two-stage up-flow anaerobic sludge blanket and downflow hanging sponge system. Water Sci. Technol. 73: 1777-1784. https://doi.org/10.2166/wst.2016.019
  21. Watari T, Thanh NT, Tsuruoka N, Tanikawa D, Kuroda K, Huong N, et al. 2016. Development of a BR-UASB-DHS system for natural rubber processing wastewater treatment. Environ. Technol. 37: 459-465. https://doi.org/10.1080/09593330.2015.1117042
  22. Nilsson JM, Riedel SA. 2011. Techniques of circuit analysis, pp. 88-143. In Horton MJ, Gilfillan A, Opaluch W, Galligan T, Disanno S, Kernan R, Fischer A, Beck K, Carney K (eds.), Electric Circuits, 9th Ed. Pearson Education, New Jersey.
  23. Morant KV, da Silva PH, de Campos-Takaki GM, Hernandez CEL. 2014. Isolation and bioelectrochemical characterization of novel fungal sources with oxidasic activity applied in situ for the cathodic oxygen reduction in microbial fuel cells. Enzyme Microb. Technol. 66: 20-27. https://doi.org/10.1016/j.enzmictec.2014.07.007

Cited by

  1. Fungi-Based Microbial Fuel Cells vol.11, pp.10, 2018, https://doi.org/10.3390/en11102827
  2. Microbial fuel cell-induced production of fungal laccase to degrade the anthraquinone dye Remazol Brilliant Blue R vol.17, pp.3, 2018, https://doi.org/10.1007/s10311-019-00876-y
  3. Microbial fuel cells coupled with the bioleaching technique that enhances the recovery of Cu from the secondary mine tailings in the bio‐electrochemical system vol.38, pp.5, 2019, https://doi.org/10.1002/ep.13181
  4. Employing Laccase-Producing Aspergillus sydowii NYKA 510 as a Cathodic Biocatalyst in Self-Sufficient Lighting Microbial Fuel Cell vol.29, pp.12, 2018, https://doi.org/10.4014/jmb.1907.07031
  5. Fungal-mediated electrochemical system: Prospects, applications and challenges vol.2, pp.None, 2021, https://doi.org/10.1016/j.crmicr.2021.100041