Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

  • Ma, Xiaojian (National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University) ;
  • Wu, Chongde (College of Light Industry, Textile and Food Engineering, Sichuan University) ;
  • Jun, Huang (College of Light Industry, Textile and Food Engineering, Sichuan University) ;
  • Zhou, Rongqing (College of Light Industry, Textile and Food Engineering, Sichuan University) ;
  • Shi, Bi (National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University)
  • Received : 2017.12.19
  • Accepted : 2018.06.10
  • Published : 2018.07.28
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Abstract

The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater. A total of six phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20), were identified as the main phyla, and Proteobacteria dominated in all the samples. Within Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater, respectively, which exhibited relatively high abundance in all samples. In addition, real-time quantitative PCR was conducted to validate the results by quantifying the abundance of the AOB and total bacteria, and similar results were obtained. Overall, the results presented in this study may provide new insights into our understanding of key microorganisms and the entire community of tannery wastewater and contribute to improving the nitrogen removal efficiency.

Keywords

References

  1. Wang YN, Zeng Y, Zhou J, Zhang W, Liao X, Shi B. 2016. An integrated cleaner beamhouse process for minimization of nitrogen pollution in leather manufacture. J. Clean Prod. 112: 2-8. https://doi.org/10.1016/j.jclepro.2015.07.060
  2. Wang Y, Zeng Y, Chai X, Liao X, He Q. 2012. Ammonia nitrogen in tannery wastewater: distribution, origin, and prevention. J. Am. Leather Chem. Assoc. 107: 40-50.
  3. Zhou J, Wang YN, Zhang W, Bi S. 2014. Nutrient balance in aerobic biological treatment of tannery wastewater. J. Am. Leather Chem. Assoc. 109: 154-160.
  4. Peng X, Guo F, Ju F, Zhang T. 2014. Shifts in the microbial community, nitrifiers and denitrifiers in the biofilm in a full-scale rotating biological contactor. Environ. Sci. Technol. 48: 8044-8052. https://doi.org/10.1021/es5017087
  5. Ye L, Zhang T, Wang T, Fang Z. 2012. Microbial structures, functions, and metabolic pathways in wastewater treatment bioreactors revealed using high-throughput sequencing. Environ. Sci. Technol. 46: 13244-13252. https://doi.org/10.1021/es303454k
  6. Zhao Y, Huang J, Hai Z, Hua Y. 2013. Microbial community and N removal of aerobic granular sludge at high COD and N loading rates. Bioresour. Technol. 143: 439-446. https://doi.org/10.1016/j.biortech.2013.06.020
  7. Zhang S, Sha C, Jiang W, Li W, Zhang D, Li J, et al. 2015. Ammonium removal at low temperature by a newly isolated heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas fluorescens wsw-1001. Environ. Technol. 36: 2488-2494. https://doi.org/10.1080/09593330.2015.1035759
  8. Amann RI, Ludwig W, Schleifer KH. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59: 143-169.
  9. Wang L, Liu J, Zhao Q, Wei W, Sun Y. 2016. Comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems. Bioresour. Technol. 211: 1-5. https://doi.org/10.1016/j.biortech.2016.03.048
  10. Waheed H, Hashmi I, Naveed AK, Khan SJ. 2013. Molecular detection of microbial community in a nitrifying-denitrifying activated sludge system. Int. Biodeterior. Biodegradation 85: 527-532. https://doi.org/10.1016/j.ibiod.2013.05.009
  11. Hu B, Zheng P, Tang C, Chen J, van der Biezen E, Zhang L, et al. 2010. Identification and quantification of anammox bacteria in eight nitrogen removal reactors. Water Res. 44: 5014-5020. https://doi.org/10.1016/j.watres.2010.07.021
  12. Ge S, Wang S, Xiong Y, Shuang Q, Li B, Peng Y. 2015. Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment: a review. Chemosphere 140: 85-98. https://doi.org/10.1016/j.chemosphere.2015.02.004
  13. Sauder LA, Peterse F, Schouten S, Neufeld JD. 2012. Lowammonia niche of ammonia-oxidizing archaea in rotating biological contactors of a municipal wastewater treatment plant. Environ. Microbiol. 14: 2589-2600. https://doi.org/10.1111/j.1462-2920.2012.02786.x
  14. Zheng X , Su Y, Li X, Xiao N, Wang D, Chen Y. 2013. Pyrosequencing reveals the key microorganisms involved in sludge alkaline fermentation for efficient short-chain fatty acids production. Environ. Sci. Technol. 47: 4262-4268. https://doi.org/10.1021/es400210v
  15. Wang B, Peng Y, Guo Y, Zhao M, Wang S. 2016. Illumina MiSeq sequencing reveals the key microorganisms involved in partial nitritation followed by simultaneous sludge fermentation, denitrification and anammox process. Bioresour. Technol. 207: 118-125. https://doi.org/10.1016/j.biortech.2016.01.072
  16. Tao Y, Li J, Rui J, Xu Z, Zhou Y, Hu X, et al. 2014. Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor. Appl. Environ. Microbiol. 80: 2254-2260. https://doi.org/10.1128/AEM.04070-13
  17. Zafra G, Taylor TD, Absalon AE, Cortes-Espinosa DV. 2016. Comparative metagenomic analysis of PAH degradation in soil by a mixed microbial consortium. J. Hazard. Mater. 318: 702-710. https://doi.org/10.1016/j.jhazmat.2016.07.060
  18. Zhang T, Shao MF, Ye L. 2012. 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME J. 6: 1137-1147. https://doi.org/10.1038/ismej.2011.188
  19. Ibarbalz FM, Figuerola ELM, Erijman L. 2013. Industrial activated sludge exhibit unique bacterial community composition at high taxonomic ranks. Water Res. 47: 3854- 3864. https://doi.org/10.1016/j.watres.2013.04.010
  20. Li D, Chen B, Zhang L, Gaur U, Ma T, Jie H, et al. 2016. The musk chemical composition and microbiota of Chinese forest musk deer males. Sci. Rep. 6: 18975. https://doi.org/10.1038/srep18975
  21. Gantner S, Andersson AF, Alonso-Saez L, Bertilsson S. 2011. Novel primers for 16S rRNA-based archaeal community analyses in environmental samples. J. Microbiol. Methods 84: 12-18. https://doi.org/10.1016/j.mimet.2010.10.001
  22. Zhang J, Lv C, Tong J, Liu J, Liu J, Yu D, et al. 2015. Optimization and microbial community analysis of anaerobic co-digestion of food waste and sewage sludge based on microwave pretreatment. Bioresour. Technol. 200: 253-261.
  23. Magoc T, Salzberg SL. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27: 2957-2963. https://doi.org/10.1093/bioinformatics/btr507
  24. Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R. 2011. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27: 2194-2200. https://doi.org/10.1093/bioinformatics/btr381
  25. Ma Q, Qu Y, Shen W, Zhang Z, Wang J, Liu Z, et al. 2015. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina highthroughput sequencing. Bioresour. Technol. 179: 436-443. https://doi.org/10.1016/j.biortech.2014.12.041
  26. Wang Z, Zhang XX, Lu X, Liu B, Li Y, Long C, et al. 2014. Abundance and diversity of bacterial nitrifiers and denitrifiers and their functional genes in tannery wastewater treatment plants revealed by high-throughput sequencing. PLoS One 9: e113603. https://doi.org/10.1371/journal.pone.0113603
  27. Yang Q, Xiong P, Ding P, Chu L, Wang J. 2015. Treatment of petrochemical wastewater by microaerobic hydrolysis and anoxic/oxic processes and analysis of bacterial diversity. Bioresour. Technol. 196: 169-175. https://doi.org/10.1016/j.biortech.2015.07.087
  28. Liang H, Ye D, Li P, Su T, Wu J, Luo L. 2016. Evolution of bacterial consortia in an integrated tannery wastewater treatment process. RSC Adv. 6: 87380-87388. https://doi.org/10.1039/C6RA19603A
  29. Zhang X, Qu Y, Ma Q, Zhang Z, Li D, Wang J, et al. 2015. Illumina MiSeq sequencing reveals diverse microbial communities of activated sludge systems stimulated by different aromatics for indigo biosynthesis from indole. PLoS One 10: e0125732. https://doi.org/10.1371/journal.pone.0125732
  30. Wang X, Hu M, Xia Y, Wen X, Ding K. 2012. Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China. Appl. Environ. Microbiol. 78: 7042-7047. https://doi.org/10.1128/AEM.01617-12
  31. Ibarbalz FM, Figuerola EL, Erijman L. 2013. Industrial activated sludge exhibit unique bacterial community composition at high taxonomic ranks. Water Res. 47: 3854-3864. https://doi.org/10.1016/j.watres.2013.04.010
  32. Shu D, He Y, Yue H, Wang Q. 2015. Microbial structures and community functions of anaerobic sludge in six fullscale wastewater treatment plants as revealed by 454 highthroughput pyrosequencing. Bioresour. Technol. 186: 163-172. https://doi.org/10.1016/j.biortech.2015.03.072
  33. Desta AF, Assefa F, Leta S, Stomeo F, Wamalwa M, Njahira M, et al. 2015. Microbial community structure and diversity in an integrated system of anaerobic-aerobic reactors and a constructed wetland for the treatment of tannery wastewater in Modjo, Ethiopia. PLoS One 10: e0128053. https://doi.org/10.1371/journal.pone.0128053
  34. Loy A, Schulz C, Lucker S, Schopfer-Wendels A, Stoecker K, Baranyi C, et al. 2005. 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order "Rhodocyclales". Appl. Environ. Microbiol. 71: 1373-1386. https://doi.org/10.1128/AEM.71.3.1373-1386.2005
  35. Mao Y, Zhang X, Xia X, Zhong H, Zhao L. 2010. Versatile aromatic compound-degrading capacity and microdiversity of Thauera strains isolated from a coking wastewater treatment bioreactor. J. Ind. Microbiol. Biotechnol. 37: 927-934. https://doi.org/10.1007/s10295-010-0740-7
  36. Naether A, Foesel BU, Naegele V, Wust PK, Weinert J, Bonkowski M, et al. 2012. Environmental factors affect acidobacterial communities below the subgroup level in grassland and forest soils. Appl. Environ. Microbiol. 78: 7398-7406. https://doi.org/10.1128/AEM.01325-12
  37. Zhuang H, Hong X, Han H, Shan S. 2016. Effect of pure oxygen fine bubbles on the organic matter removal and bacterial community evolution treating coal gasification wastewater by membrane bioreactor. Bioresour. Technol. 221: 262-269. https://doi.org/10.1016/j.biortech.2016.09.029
  38. Gonzalez-Martinez A, Rodriguez-Sanchez A, Rodelas B, Abbas BA, Martinez-Toledo MV, Van Loosdrecht M, et al. 2015. 454-Pyrosequencing analysis of bacterial communities from autotrophic nitrogen removal bioreactors utilizing universal primers: effect of annealing temperature. BioMed. Res. Int. 2015: 1-12.
  39. Liu Z, Frigaard N-U, Vogl K, Iino T, Ohkuma M, Overmann J, et al. 2012. Complete genome of Ignavibacterium album, a metabolically versatile, flagellated, facultative anaerobe from the phylum Chlorobi. Front. Microbiol. 3: 1-15.
  40. Tian M, Zhao F, Shen X, Chu K, Wang J, Chen S, et al. 2015. The first metagenome of activated sludge from full-scale anaerobic/anoxic/oxic (A2O) nitrogen and phosphorus removal reactor using Illumina sequencing. J. Environ. Sci. 35: 181-190. https://doi.org/10.1016/j.jes.2014.12.027
  41. Kamagata Y, Mikami E. 1991. Isolation and characterization of a novel thermophilic Methanosaeta strain. Int. J. Syst. Evol. Microbiol. 41: 191-196.
  42. Siripong S, Rittmann BE. 2007. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants. Water Res. 41: 1110-1120. https://doi.org/10.1016/j.watres.2006.11.050
  43. Bai Y, Sun Q, Wen D, Tang X. 2012. Abundance of ammonia-oxidizing bacteria and archaea in industrial and domestic wastewater treatment systems. FEMS Microbiol. Ecol. 80: 323-330. https://doi.org/10.1111/j.1574-6941.2012.01296.x
  44. Duan L, Song Y, Xia S, Hermanowicz SW. 2013. Characterization of nitrifying microbial community in a submerged membrane bioreactor at short solids retention times. Bioresour. Technol. 149: 200-207. https://doi.org/10.1016/j.biortech.2013.09.050
  45. Martens-Habbena W, Berube PM, Urakawa H, de La Torre JR, Stahl DA. 2009. Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria. Nature 461: 976-979. https://doi.org/10.1038/nature08465
  46. Wuchter C, Abbas B, Coolen MJ, Herfort L, van Bleijswijk J, Timmers P, et al. 2006. Archaeal nitrification in the ocean. Proc. Natl. Acad. Sci. USA 103: 12317-12322. https://doi.org/10.1073/pnas.0600756103
  47. Zhang T, Ye L, Tong AHY, Shao M-F, Lok S. 2011. Ammoniaoxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors. Appl. Microbiol. Biotechnol. 91: 1215-1225. https://doi.org/10.1007/s00253-011-3408-y

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