The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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A Study of the Effect on Obesity and dyslipidemia in Kidney-hypofunction Animal Model Induced by Unilateral Ureteral Obstruction

신장절제로 유발한 신약(腎弱) 동물 모델에서의 비만 및 지질대사에 대한 영향 평가

  • Kwak, Jinyoung (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon University) ;
  • Park, Junghwan (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon University) ;
  • Koh, Youngmee (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon University) ;
  • Ahn, Taekwon (Dept. of Sasang Constitutional Medicine, College of Korean Medicine, Daejeon University)
  • 곽진영 (대전대학교 한의과대학 사상체질의학교실) ;
  • 박정환 (대전대학교 한의과대학 사상체질의학교실) ;
  • 고영미 (대전대학교 한의과대학 사상체질의학교실) ;
  • 안택원 (대전대학교 한의과대학 사상체질의학교실)
  • Received : 2018.03.15
  • Accepted : 2018.06.04
  • Published : 2018.06.30
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Abstract

Objectives: The objective of this study is to develop a new animal model with Kidney-hypofunction for Sasang Constitutional Medicine, especially for partial Soyangin(one of four constitution which has good digestive function and poor renal function) by Unilateral Ureteral Obstruction, and to estimate the factor related to obesity, dyslipidemia, and metabolic syndrome. Methods: The C57BL/6J mice were divided into 3 groups : normal group, high fat diet(HFD) control group, and HFD group with Unilateral Ureteral Obstruction(UUO). Then, the HFD control group and the experimental group were fed with high fat diet for 6 weeks. Food intake and body weight were measured at regular time by week. After the final experiment, blood was gathered for bloodchemical examination and organs(liver, fatty tissue) were remoed, weighted, and mRNA was analyzed with real-time PCR. Results: The weight growth rate with High fat diet went down by 8.35% in experimental group and had similar FER with the normal group, while HFD control group had higher weight growth rate and FER than any other groups. Also The experimental group had lower triglyceride and LDL cholesterol rate and higher glucose rate in serum. and in mRNA expression, GLUT-9, the protein related to excretion of uric acid and metabolic syndrome, expressed lower rate than that of HFD control group. and IL-6, a kind of cytokine related to obesity and metabolic syndrome, expressed more than HFD control group. Conclusions: It was found that Kidney-hypofunction animal-experimental model is susceptible to metabolic syndrome.

Keywords

References

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