Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice

  • Ahmadi, Hamid (Department of Anatomy, Medical School, Tehran University of Medical Sciences) ;
  • Fathi, Fardin (Cellular and Molecular Research Center, School of Medicine, Kurdistan University of Medical Sciences) ;
  • Moeini, Ashraf (Department of Obstetrics and Gynecology, Arash Women's Hospital, Tehran University of Medical Sciences) ;
  • Amidi, Fardin (Department of Anatomy, Medical School, Tehran University of Medical Sciences) ;
  • Sobhani, Aligholi (Department of Anatomy, Medical School, Tehran University of Medical Sciences)
  • Received : 2018.03.30
  • Accepted : 2018.06.08
  • Published : 2018.06.30


Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. Methods: Mice (6-8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as $mean{\pm}standard$ deviation. Results: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were $59.70{\pm}22.30$ and $54.70{\pm}18.22$, respectively (p= 0.82). Conclusion: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.



Supported by : Tehran University of Medical Sciences


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