Clinical and Experimental Vaccine Research

  • 제7권1호
  • /
  • Pages.82-86
  • /
  • 2018
  • /
  • 2287-3651(pISSN)
  • /
  • 2287-366X(eISSN)
대한백신학회 (The Korean Vaccine Society)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

  • Yu, Min-Ah (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Jeong, Hye Won (Department of Internal Medicine, Chungbuk National University Hospital) ;
  • Park, Su-Jin (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kim, Young-Il (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kwon, Hyeok-Il (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kim, Eun-Ha (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Si, Young-Jae (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Yu, Kwang Min (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Robles, Norbert John (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Han, Hae Jung (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Choi, Young Ki (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine)
  • 투고 : 2017.12.30
  • 심사 : 2018.01.15
  • 발행 : 2018.01.31
⟨ 이전 논문 다음 논문 ⟩

초록

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.

키워드

과제정보

연구 과제 주관 기관 : Korea Health Industry Development Institute

참고문헌

  1. Jin C, Liang M, Ning J, et al. Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/ BL6 mouse model. Proc Natl Acad Sci U S A 2012;109: 10053-8. https://doi.org/10.1073/pnas.1120246109
  2. Liu S, Chai C, Wang C, et al. Systematic review of severe fever with thrombocytopenia syndrome: virology, epidemiology, and clinical characteristics. Rev Med Virol 2014; 24:90-102. https://doi.org/10.1002/rmv.1776
  3. Yu XJ, Liang MF, Zhang SY, et al. Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 2011;364:1523-32. https://doi.org/10.1056/NEJMoa1010095
  4. Takahashi T, Maeda K, Suzuki T, et al. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis 2014;209:816-27. https://doi.org/10.1093/infdis/jit603
  5. Kim KH, Yi J, Kim G, et al. Severe fever with thrombocytopenia syndrome, South Korea, 2012. Emerg Infect Dis 2013; 19:1892-4.
  6. Zhang YZ, Xu J. The emergence and cross species transmission of newly discovered tick-borne Bunyavirus in China. Curr Opin Virol 2016;16:126-31. https://doi.org/10.1016/j.coviro.2016.02.006
  7. Niu G, Li J, Liang M, et al. Severe fever with thrombocytopenia syndrome virus among domesticated animals, China. Emerg Infect Dis 2013;19:756-63.
  8. Zhao L, Zhai S, Wen H, et al. Severe fever with thrombocytopenia syndrome virus, Shandong Province, China. Emerg Infect Dis 2012;18:963-5.
  9. Fu Y, Li S, Zhang Z, et al. Phylogeographic analysis of severe fever with thrombocytopenia syndrome virus from Zhoushan Islands, China: implication for transmission across the ocean. Sci Rep 2016;6:19563. https://doi.org/10.1038/srep19563
  10. Rudd RJ, Trimarchi CV. Development and evaluation of an in vitro virus isolation procedure as a replacement for the mouse inoculation test in rabies diagnosis. J Clin Microbiol 1989;27:2522-8.
  11. Xu B, Liu L, Huang X, et al. Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus. PLoS Pathog 2011;7:e1002369. https://doi.org/10.1371/journal.ppat.1002369
  12. Fukuma A, Fukushi S, Yoshikawa T, et al. Severe fever with thrombocytopenia syndrome virus antigen detection using monoclonal antibodies to the nucleocapsid protein. PLoS Negl Trop Dis 2016;10:e0004595. https://doi.org/10.1371/journal.pntd.0004595
  13. Yu F, Du Y, Huang X, et al. Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA. Virol J 2015;12:117. https://doi.org/10.1186/s12985-015-0350-0
  14. Lee H, Kim EJ, Song JY, et al. Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera. J Vet Sci 2016;17:307-14. https://doi.org/10.4142/jvs.2016.17.3.307
  15. Chen S, Sun L, Liu Y, et al. Development of human antibodies against the Gn protein of severe fever with thrombocytopenia syndrome virus. Bing Du Xue Bao 2015;31:24-9.
  16. Yun SM, Park SJ, Park SW, et al. Molecular genomic characterization of tick- and human-derived severe fever with thrombocytopenia syndrome virus isolates from South Korea. PLoS Negl Trop Dis 2017;11:e0005893. https://doi.org/10.1371/journal.pntd.0005893
  17. Harper S, Speicher DW. Expression and purification of GST fusion proteins. Curr Protoc Protein Sci 2008;Chapter 6:Unit 6.6.
  18. Palmer I, Wingfield PT. Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Curr Protoc Protein Sci 2004;Chapter 6:Unit 6.3.
  19. Facility HF. Protocol for immunofluorescence staining of adhesion cells [Internet]. Hong Kong: Li Ka Shing Faculty of Medicine; 2017 [cited 2017 Dec 2]. Available from: https: //www.med. hku.hk/corefac/downloads/immunostaining% 20protocol%20cell%20culture.pdf.

피인용 문헌

  1. Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Phlebovirus in Domesticated Deer in South Korea vol.34, pp.5, 2018, https://doi.org/10.1007/s12250-019-00137-w
  2. Cross-genotype protection of live-attenuated vaccine candidate for severe fever with thrombocytopenia syndrome virus in a ferret model vol.116, pp.52, 2019, https://doi.org/10.1073/pnas.1914704116