Clinical and Experimental Vaccine Research

The Korean Vaccine Society (대한백신학회)
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Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis

  • Yu, Min-Ah (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Jeong, Hye Won (Department of Internal Medicine, Chungbuk National University Hospital) ;
  • Park, Su-Jin (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kim, Young-Il (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kwon, Hyeok-Il (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Kim, Eun-Ha (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Si, Young-Jae (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Yu, Kwang Min (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Robles, Norbert John (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Han, Hae Jung (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Choi, Young Ki (Department of Microbiology and Medical Research Institute, Chungbuk National University College of Medicine)
  • Received : 2017.12.30
  • Accepted : 2018.01.15
  • Published : 2018.01.31
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Abstract

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.

Keywords

Acknowledgement

Supported by : Korea Health Industry Development Institute

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