한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제51권3호
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  • Pages.328-331
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  • 2018
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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신경괴사증바이러스(nervous necrosis virus, RGNNV genotype)에 대한 단클론 항체 생산

Production of Monoclonal Antibodies Against Nervous Necrosis Virus (NNV, RGNNV genotype)

  • 김위식 (전남대학교 수산생명의학과) ;
  • 김시우 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Si-Woo (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 투고 : 2018.05.15
  • 심사 : 2018.06.14
  • 발행 : 2018.06.30
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초록

We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against nervous necrosis virus (NNV, RGNNV genotype). We established six hybridoma clones secreting MAbs against NNV antigen: 2B1, 2B11, 2C12, 13C1-1, 13C1-2 and 14D11. All six MAbs belonged to the IgG2a isotype with a kappa light chain and their reactivity recognized against the 41 kDa coat protein of NNV by Western blot analysis. The affinity constants of the six MAbs were measured by enzyme-linked immunosorbent assay (ELISA). All six MAbs reacted with two NNV isolates (SgNag05 and Gemunodo06), while no reactivity was observed with five know fish viruses, namely marine birnavirus, infectious pancreatic necrosis virus, viral hemorrhagic septicemia virus, hirame rhabdovirus, and infectious hematopoietic necrosis virus. Moreover, high ELISA optical density (OD) values (0.87-1.42) were observed in the brain tissues of NNV-infected sevenband grouper, while low OD values (less than 0.12) were recorded in the brain tissues of uninfected fish. These results suggest that these six MAbs are highly competent and useful for the detection of NNV with the RGNNV genotype.

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참고문헌

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