Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Characteristics of Vibrio anguillarum Isolated from Seawater Cultured Rainbow Trout Oncorhynchus mykiss in Korea

해수 사육 무지개송어(Oncorhynchus mykiss)에서 분리된 Vibrio anguillarum의 특성 분석

  • Chun, Hye-Jin (Pathology Division, National Institute of Fisheries Science) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Cho, Mi-Young (Pathology Division, National Institute of Fisheries Science) ;
  • Jung, Sung-Hee (Pathology Division, National Institute of Fisheries Science) ;
  • Han, Hyun-Ja (Pathology Division, National Institute of Fisheries Science)
  • 천혜진 (국립수산과학원 병리연구과) ;
  • 김위식 (전남대학교 수산생명의학과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 한현자 (국립수산과학원 병리연구과)
  • Received : 2018.02.06
  • Accepted : 2018.04.20
  • Published : 2018.06.30
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Abstract

From 2014 to 2017, mortalities of seawater-cultured rainbow trout Oncorhynchus mykiss, were observed in the Goheung and Jeju areas of Korea, with Vibrio anguillarum (seven strains: RT1, 2, 3, 4, 5, 6, and 7) identified as the etiological agent. The phenotypic (based on API 20NE, API ZYM, and E-test kits), serotypic (slide agglutination tests with O1, O2, O3, O4, and O7 antisera), and genotypic (16S rRNA and ompU sequencing) characteristics of the seven RT strains were analyzed and compared to those of seven additional V. anguillarum stains (SF, isolated from sweet fish; FM, isolated from flathead mullet; ATCC43305; ATCC43311; ATCC43307; ATCC43308; and KCTC2711). The phenotypes of the RT strains showed variance, while the slide agglutination tests of the RT1-7, SF, and FM strains all showed positive reactions with serotype O1 antiserum. The 16S rRNA and ompU sequences of the RT1-7, SF, and FM strains were affiliated with V. anguillarum ATCC43305 (Serotype O1), but the ompU sequence of the SF strain differed from those of the RT1-7, FM, and ATCC43311 strains, including one amino acid substitution. We thus confirmed that serotype O1 V. anguillarum, with multiple phenotypes, continues to infect seawater-cultured rainbow trout in Korea.

Keywords

References

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