한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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Antioxidant Activity of Manno-oligosaccharides Derived from the Hydrolysis of Polymannan by Extracellular Carbohydrase of Bacillus N3

  • Amna, Kashif Shaheen (Department of Biomedical Sciences, Gachon University) ;
  • Park, So Yeon (Department of Biomedical Sciences, Gachon University) ;
  • Choi, Min (Department of Biomedical Sciences, Gachon University) ;
  • Kim, Sang Yeon (Department of Biomedical Sciences, Gachon University) ;
  • Yoo, Ah Young (Department of Biomedical Sciences, Gachon University) ;
  • Park, Jae Kweon (Department of Biomedical Sciences, Gachon University)
  • 투고 : 2018.04.30
  • 심사 : 2018.06.07
  • 발행 : 2018.06.30
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초록

The aim of this study is to elucidate the biochemical properties of manno-oligosaccharides (MOS) hydrolyzed by extracellular enzyme of Bacillus N3. We strived to characterize the biochemical properties of MOS since N3 can effectively hydrolyzed natural polymannans such as galactomannan (GM) and konjac (glucomannan, KM), respectively. The hydrolysis of GM and KM was applied by the strain N3 in terms of reducing sugars and the highest production of reducing sugars was estimated to be about 750 mg/L and 370 mg/L respectively, which were quantified after 7 days of cultivation in the presence of both substrates. Hydrolysates derived from the hydrolysis of KM showed the significant antioxidant activity based on DPPH and ABTS radical scavenging activity with increasing of tyrosinase inhibitory activity. On the other hand, hydrolysates derived from the hydrolysis of GM showed only ABTS radical scavenging activity without showing significant changes on tyrosinase inhibitory activity. Our data suggest that those biological characteristics may be depend on the primary structure and the size of MOS, which may be useful as potent additives for diet foods.

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