Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Antioxidant Activity of Manno-oligosaccharides Derived from the Hydrolysis of Polymannan by Extracellular Carbohydrase of Bacillus N3

  • Amna, Kashif Shaheen (Department of Biomedical Sciences, Gachon University) ;
  • Park, So Yeon (Department of Biomedical Sciences, Gachon University) ;
  • Choi, Min (Department of Biomedical Sciences, Gachon University) ;
  • Kim, Sang Yeon (Department of Biomedical Sciences, Gachon University) ;
  • Yoo, Ah Young (Department of Biomedical Sciences, Gachon University) ;
  • Park, Jae Kweon (Department of Biomedical Sciences, Gachon University)
  • Received : 2018.04.30
  • Accepted : 2018.06.07
  • Published : 2018.06.30
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Abstract

The aim of this study is to elucidate the biochemical properties of manno-oligosaccharides (MOS) hydrolyzed by extracellular enzyme of Bacillus N3. We strived to characterize the biochemical properties of MOS since N3 can effectively hydrolyzed natural polymannans such as galactomannan (GM) and konjac (glucomannan, KM), respectively. The hydrolysis of GM and KM was applied by the strain N3 in terms of reducing sugars and the highest production of reducing sugars was estimated to be about 750 mg/L and 370 mg/L respectively, which were quantified after 7 days of cultivation in the presence of both substrates. Hydrolysates derived from the hydrolysis of KM showed the significant antioxidant activity based on DPPH and ABTS radical scavenging activity with increasing of tyrosinase inhibitory activity. On the other hand, hydrolysates derived from the hydrolysis of GM showed only ABTS radical scavenging activity without showing significant changes on tyrosinase inhibitory activity. Our data suggest that those biological characteristics may be depend on the primary structure and the size of MOS, which may be useful as potent additives for diet foods.

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References

  1. Ding, J., Zhu, H., Ye, Y., Li, J., Han, N., Wu, Q., Huang, Z., and Zee, M. 2018. A thermostable and alkaline GDSL-motif esterase from Bacillus sp. K91: crystallization and X-ray crystallographic analysis. Acta Crystallogr F. Struct. Biol. Commun,. 74 (Pt 2), 117-121.
  2. Lisdiyanti, P., Suyanto, E., Gusmawati, N., and Rahayu, W. 2012. Isolation and characterization of cellulose produced by cellulolytic bacteria from peat soil of ogan komering Ilir, South Sumatera. Int. J. Environ. Bioen., 3(3), 145-153.
  3. Sajith, S., Sreedevi, S., Priji, P., Unni, K. N., and Benjamin, S. 2014. Production and partial purification of cellulase from a novel fungus, Aspergillus flavus BS1. Annals Microbiol., 64(2), 763-771. https://doi.org/10.1007/s13213-013-0711-0
  4. Paz, A., Carballo, J., Perez, M. J., and Dominguez, J. M. 2016. Bacillus aryabhattai BA03: a novel approach to the production of natural value-added compounds. World J. Microbiol Biotech., 32, 159-165. https://doi.org/10.1007/s11274-016-2113-5
  5. Chawla, P. R., Bajaj, I. B., Survase, S., and Singhal, R. S. 2009. Microbial cellulose: Fermentative production and application. Food Technol. Biotechnol., 47, 107-124
  6. Parab, P., Khandeparker, R., Amberkar, U., and Khodse, V. 2017. Enzymatic saccharification of seaweeds into fermentable sugars by xylanase from marine Bacillus sp. strain BT21. Biotech., 7(5), 291-296.
  7. Rodrigues, D. F., and Elimelech, M. 2009. Role of type 1 fimbriae and mannose in the development of Escherichi a coli K12 biofilm: from initial cell adhesion to biofilm formation. Biofouling, 25(5), 401-411. https://doi.org/10.1080/08927010902833443
  8. StOnge, M. P., Salinardi, T., Herron-Rubin, K., and Black, R.M. 2012. A weight-loss diet including coffee-derived mannooligosaccharides enhances adipose tissue loss in overweight men but not women. Obesity, 20(2), 343-348. https://doi.org/10.1038/oby.2011.289
  9. Nelson, N. 1944. A photometric adaptation of the somogyi method for the determination of glucose. J. Biol. Chem., 153(2), 375-380.
  10. Pomerantz, S. H. 1966. The tyrosine hydroxylase activity of mammalian tyrosinase. J. Biol. Chem., 241(1), 161-168.
  11. Amna, S., Lee, B. M., Lee, C. G., Hwang, Y. J., and Park, J. K. 2016. Degradation of polysaccharide in fenton oxidative cleavage reaction and their antioxidant activity. J. Chitin Chitosan, 21(1), 20-25. https://doi.org/10.17642/jcc.21.1.4
  12. Amir, R. M., Anjum, F. M., Khan, M. I., Khan. M. R., Pasha, I., and Nadeem, M. 2013. Application of fourier transform infrared (FTIR) spectroscopy for the identificatio n of wheat varieties. J. Food Sci. Tech., 50(5), 1018-1023. https://doi.org/10.1007/s13197-011-0424-y
  13. Chen, C. W., and Ho, C.T. 1995. Antioxidant properties of polyphenols extracted from green and black teas. J. Food Lipids, 2(1), 35-46. https://doi.org/10.1111/j.1745-4522.1995.tb00028.x
  14. Cano, A., Acosta, M., and Arnao, M. B. 2000. A method to measure antioxidant activity in organic media: applicati on to lipophilic vitamins. Redox. Rep., 5(6), 365-370. https://doi.org/10.1179/135100000101535933
  15. Videira, I. F., Moura, D. F., and Magina, S. 2013. Mechanisms regulating melanogenesis. An. Bras. Dermatol., 88 (1), 76-83. https://doi.org/10.1590/S0365-05962013000100009
  16. Kamkaen, N., Mulsri, N., and Treesak, C. 2007. Screening of some tropical vegetables for anti-tyrosinase activity. J. Thail. Pharm. Health Sci., 2(1), 15-19.
  17. Briganti, S., Camera, E., and Picardo, M. 2003. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res., 16(2), 101-110. https://doi.org/10.1034/j.1600-0749.2003.00029.x
  18. Khatib, S., Nerya, O., Musa, R., Shmuel, M., and Tamir, zS. 2005. Vaya JChalcones as potent tyrosinase inhibitors: the importance of a 2,4-substituted resorcinol moiety. Bioorganic & Medicinal Chem., 13(2), 433-441. https://doi.org/10.1016/j.bmc.2004.10.010
  19. Horn, S. J., Vaaje-Kolstad, G., Westereng, B., and Eijsink, V. G. H. 2012. Novel enzymes for the degradation of cellulose. Biotech. Biofuels, 5, 45-45. https://doi.org/10.1186/1754-6834-5-45
  20. Devreese, M., Girgis, G. N., Tran, S. T., DeBaere, S., DeBacker, P., Croubels, S., and Smith, T. K. 2014. The effects of feed-borne Fusarium mycotoxins and glucomannan in turkey poults based on specific and non-specific parameters. Food Chem. Toxicol., 63, 69-75. https://doi.org/10.1016/j.fct.2013.10.044
  21. Shibata, N., Kobayashi, H., Takahashi, S., Okawa, Y., Hisamichi, K., Suzuki, S., and Suzuki, S. 1991. Structural study on a phosphorylated mannotetraose obtained from the phosphomannan of Candida albicans NIH B-792 strai n by acetolysis. Arch. Biochem. Biophys., 290(2), 535-542. https://doi.org/10.1016/0003-9861(91)90578-7
  22. Chang, T. S. 2012. Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity. Materials, 5(9), 1661-1685. https://doi.org/10.3390/ma5091661
  23. Park, J. K. 2013. Purification and characterisation of a novel alkalophilic ${\alpha}$-D-mannosidase from Pseudomonas fluorescens. Biocontrol Sci.Technol., 23, 1324-1335. https://doi.org/10.1080/09583157.2013.838209