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Complete genome sequence of Bacillus licheniformis 14ADL4 exhibiting resistance to clindamycin

Clindamycin 내성 Bacillus licheniformis 14ADL4의 유전체 염기서열

  • Jeong, Do-Won (Department of Food and Nutrition, Dongduk Women's University) ;
  • Lee, Byunghoon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University)
  • 정도원 (동덕여자대학교 식품영양학과) ;
  • 이병훈 (경기대학교 식품생물공학과) ;
  • 이종훈 (경기대학교 식품생물공학과)
  • Received : 2018.05.23
  • Accepted : 2018.05.28
  • Published : 2018.06.30

Abstract

Clindamycin resistant Bacillus licheniformis 14ADL4 was isolated from doenjang, a Korean high-salt-fermented soybean food. Strain 14ADL4 contains a single circular 4,332,232 bp chromosome with a G + C content of 45.86%. The complete genome of strain 14ADL4 includes lmrA and lmrB homologs may confer resistance to clindamycin.

Clindamycin 내성 Bacillus licheniformis 14ADL4 균주는 된장으로부터 분리되었다. 14ADL4 균주의 유전체분석 결과, GC 함량 45.86%, 약 4.3 Mb 크기의 염색체가 확인되었고, 유전체 상의 lmrA와 lmrB 추정 유전자가 clindamycin 내성에 관여할 것으로 예상된다.

Keywords

References

  1. Clinical and Laboratory Standards Institute. 2007. Performance standards for antimicrobial susceptibility testing. Seventeenth informational supplement. CLSI, Wayne, PA, USA.
  2. Delcher, A.L., Bratke, K.A., Powers, E.C., and Salzberg, S.L. 2007. Identifying bacterial genes and endosymbiont DNA with glimmer. Bioinformatics 23, 673-679. https://doi.org/10.1093/bioinformatics/btm009
  3. Jeong, D.W., Jeong, M., and Lee, J.H. 2017. Antibiotic susceptibilities and characteristics of Bacillus licheniformis isolates from traditional Korean fermented soybean foods. LWT-Food Sci. Technol. 75, 565-568. https://doi.org/10.1016/j.lwt.2016.10.001
  4. Jeong, D.W., Kim, H.R., Jung, G., Han, S., Kim, C.T., and Lee, J.H. 2014. Bacterial community migration in the ripening of doenjang, a traditional Korean fermented soybean food. J. Microbiol. Biotechnol. 24, 648-660. https://doi.org/10.4014/jmb.1401.01009
  5. Kim, T.W., Lee, J.H., Kim, S.E., Park, M.H., Chang, H.C., and Kim, H.Y. 2009. Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 131, 265-271. https://doi.org/10.1016/j.ijfoodmicro.2009.03.001
  6. Leclercq, R. 2002. Mechanisms of resistance to macrolides and lincosamides: nature of the resistance elements and their clinical implications. Clin. Infect. Dis. 34, 482-492. https://doi.org/10.1086/324626
  7. Lee, J.H., Kim, T.W., Lee, H., Chang, H.C., and Kim, H.Y. 2010. Determination of microbial diversity in meju, fermented cooked soya beans, using nested PCR-denaturing gradient gel electrophoresis. Lett. Appl. Microbiol. 51, 388-394. https://doi.org/10.1111/j.1472-765X.2010.02906.x
  8. Murata, M., Ohno, S., Kumano, M., Yamane, K., and Ohki, R. 2003. Multidrug resistant phenotype of Bacillus subtilis spontaneous mutants isolated in the presence of puromycin and lincomycin. Can. J. Microbiol. 49, 71-77. https://doi.org/10.1139/w03-014
  9. Tatusov, R.L., Koonin, E.V., and Lipman, D.J. 1997. A genomic perspective on protein families. Science 278, 631-637. https://doi.org/10.1126/science.278.5338.631

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